Zfp37 (NM_009554) Mouse Tagged ORF Clone

CAT#: MG209207

Zfp37 (tGFP-tagged) - Mouse zinc finger protein 37 (Zfp37)

Product Images

Circular map for MG209207

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Zfp37
• Synonyms: Tzn; Zfp-37
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209207 representing NM_009554
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACATCCGAGCCTGCGGAAAGCGATGCGGAATGGGAGCAGCTGGAACCTGTGCAGAGAGATGTGT
  ACAAGGATACGAAGCTAGAGAACTGCAGCAATCCAGCCTCCATGGGAAATCAAGATCCCAAACAAGACAT
  AGTCTCCGTGTTGGAAGAAGAAGAGCCATCATCGGGAAAGGGGAAAAAAGCCAGCCCAAGTAGTCTGAAA
  AAAATAGCAAGGCCCAAGACAGCAGGAACAAGTGCAAAACTCCAACAAGATGATGAGCATAGGGAGGAAA
  AGCAGAAGTCCCAAAGCAAACTTACTAAGGAAGTGACACTCAGGAAGAAAAGTTCCAACAGCAAGAAAAG
  CAGTGAGTATGGTTTGTTGGAGAACAAAAGTCTCCACTCAAAACACACTCCTTCCGAGAAAAAACTGCTT
  AAGTCCAGTTCCCGTGGGAAGAACTCGAATCAGAATTCAGACTCTCTGAAAAAGAAACCTGACACAGCTA
  ATGACCACAGGAAATCACTCAGCCATTCTGCATCTGATGTGAACAAAGATGAAATTCCAACTAGAAAGAA
  ATGCGACAAGTTACCCAACAATAAGTTGTCTGATAAAGGTGACAAAAACCAAACCAGCAAAAAATGTGAG
  AAAGTATGCCGTCATAGTGCATCCCATACCAAGGAAGACAAAATTCAGACCGGGGAGAAACGGAAATCAC
  ACTGCCGTACTCCATCTAAACCTGAAAAAGCCCCAGGTTCTGGGAAACCTTATGAATGTAACCACTGTGG
  GAAGGTCCTCAGCCATAAACAGGGACTCCTTGACCATCAAAGAACTCACACTGGGGAGAAACCATATGAA
  TGTAATGAATGTGGGATAGCTTTCAGCCAGAAGTCCCACCTTGTTGTACATCAGAGAACTCACACTGGGG
  AAAAACCATACGAGTGTGAACAGTGTGGCAAAGCACACGGACATAAACATGCCCTCACTGACCATCTAAG
  AATCCATACTGGAGAAAAGCCCTACAAATGTAATGAATGTGGCAAAACGTTTAGACACAGCTCAAACCTT
  ATGCAACACCTAAGATCTCACACGGGTGAGAAGCCGTATGAATGTAAGGAATGTGGCAAATCCTTTAGAT
  ATAATTCATCTCTTACTGAACATGTGAGAACACACACAGGTGAAATACCATACGAATGTAACGAATGTGG
  CAAAGCTTTCAAGTATGGCTCATCCCTGACTAAACATATGCGGATTCATACAGGGGAGAAACCATTTGAA
  TGTAATGAATGTGGGAAAACTTTTAGCAAAAAGTCACACCTAGTTATACATCAAAGAACTCATACAAAGG
  AGAAACCTTATAAATGTGATGAGTGTGGGAAAGCCTTTGGACATAGCTCATCTCTTACCTACCATATGAG
  AACTCATACAGGTGACTGCCCCTTTGAATGTAATCAATGTGGTAAGGCCTTTAAACAGATTGAAGGCCTT
  ACCCAACACCAGAGAGTTCACACAGGGGAGAAACCCTATGAGTGTGTTGAATGTGGGAAAGCCTTTAGTC
  AGAAGTCACACCTCATCGTACACCAGAGAACTCATACAGGGGAGAAACCCTTTGAATGTTATGAGTGTGG
  AAAAGCCTTCAATGCAAAATCACAACTTGTTATTCATCAGAGATCCCACACTGGAGAGAAACCCTATGAA
  TGTATTGAATGTGGTAAAGCCTTCAAGCAAAATGCCTCTCTTACCAAACATATGAAAATTCACTCAGAAG
  AACAATCTGAGGAAGAAGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209207 representing NM_009554
  Red=Cloning site Green=Tags(s)
  
  MATSEPAESDAEWEQLEPVQRDVYKDTKLENCSNPASMGNQDPKQDIVSVLEEEEPSSGKGKKASPSSLK
  KIARPKTAGTSAKLQQDDEHREEKQKSQSKLTKEVTLRKKSSNSKKSSEYGLLENKSLHSKHTPSEKKLL
  KSSSRGKNSNQNSDSLKKKPDTANDHRKSLSHSASDVNKDEIPTRKKCDKLPNNKLSDKGDKNQTSKKCE
  KVCRHSASHTKEDKIQTGEKRKSHCRTPSKPEKAPGSGKPYECNHCGKVLSHKQGLLDHQRTHTGEKPYE
  CNECGIAFSQKSHLVVHQRTHTGEKPYECEQCGKAHGHKHALTDHLRIHTGEKPYKCNECGKTFRHSSNL
  MQHLRSHTGEKPYECKECGKSFRYNSSLTEHVRTHTGEIPYECNECGKAFKYGSSLTKHMRIHTGEKPFE
  CNECGKTFSKKSHLVIHQRTHTKEKPYKCDECGKAFGHSSSLTYHMRTHTGDCPFECNQCGKAFKQIEGL
  TQHQRVHTGEKPYECVECGKAFSQKSHLIVHQRTHTGEKPFECYECGKAFNAKSQLVIHQRSHTGEKPYE
  CIECGKAFKQNASLTKHMKIHSEEQSEEED
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009554
• ORF Size: 1770 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009554.4, NP_033580.3
• RefSeq Size: 3579 bp
• RefSeq ORF: 1773 bp
• Locus ID: 22696
• Cytogenetics: 4 33.04 cM
• Gene Summary: May have a role in regulating spermiogenesis.[UniProtKB/Swiss-Prot Function]