Taf1b (NM_020614) Mouse Tagged ORF Clone
CAT#: MG209153
- TrueORF®
Taf1b (tGFP-tagged) - Mouse TATA box binding protein (Tbp)-associated factor, RNA polymerase I, B (Taf1b)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_020614" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Taf1b |
Synonyms | 4930408G01Rik; A230108M10Rik; p63; TAFI68; Tafi86 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG209153 representing NM_020614
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTGGAGGAGGTGAAAGCGTTCAGAGACCGCTGTTCTCAGTGTGCTGCTGTCTCCTGGGGCCTTA CCGATGAAGGCAAATATTACTGTACTTCCTGCCACAATGTCACAGATCGATCTGAGGAAGTGGTGAGCGC TGCAGATATCCCCAACACCAAGATCAACTCCATCAACAGGGGGCTGAGACAGAGAAGCAAGCATGAGAAA GGCTGGGATTGGTATGTGTGTGAAGGTTTCCAGTGCATACTTTATCACCAAGCAAAAGCCCTGGAGACCC TCGGAGTAAGCCCAGAACTAAAGAATGAAGTCTTACACAATTTTTGGAAGCGCTACCTTCAGAAGAGCAA ACAGGCCTATTGTAAAAACCCAGTTCGTACAAGTGGAAGGAAAGCCAAGGTATTAGAAGACAGTGTGCAG TCCTCGGACTTGGGCAGTGATCTTGAGCTGCTAAGTGATACCACATGCCCCCTTGAAAGTGAAGCAGAGT TCCAATCTGACCCTCAGATTCCAAAGCCTTTCCCTGTCACCAAAGGATCCCCAAAGTCAGCGTCTGTCTG CTCTGGCTCTGTTGATGGAGTGGAGTACTCCGAGCGGAAGGAGAAGGGGCTTGTGAAGATGACGGTGCCC AGGACACTTGCTCTCTGCTCTCTCTCCTTGCTCTGGCAGAGGGAAACAATTACAGTTTCAGACCTTTTGA GGTTTGTTGAAGAGGATCATATTCCGTACATAAATGCTTTTAAGCTTTTTCCAGAAGAGATGAAAGTGTA TGGTCGTGACAAAGGCATCTTTGCTATAGAGTCTTGGCCTGACTATGAAGACATCTATAAAAACATGATT GAACTTGCCATATTTTTAGATTTGCCTCGTTTCCCAGACATAACGGAAGACTGCTACCTCCATCCCAACA CCTTGTGTATGAAATACTTACTGGAGGTCAATCTCCCTGATGAAATGCACACTTTAACCTGTCAAGTAGT AAAGTTGACTGGAATTGGGGAAGTGGATTTTCTGACGTTTGATCCTATAGCTAAAACGAAAAGGACAGTT AAATATGATGTGCAGGCTATGGCTGTCATTGTGGTTGTGTTGAAGCTGCTGTTTTTGCTGGATGACAAGC TGGAATGGTCTTACTCAGATCTTGCTGAAGCGTACAACGAACAACACAGAGAAGACACGCCCCAGTTTGA TTTCAGAAAATGGTACCAAGTTATGAAGAAAACTTTTGATGAGAAAAGACGAAAATGGGAAGAAGCGAGG GCCAGGTATGCTTGGAAAACCAAAAGGCCACTCTACCGTTCACACATCGATAAGTCCGTCGCATATAAGA GAAGAAAAATGGTGGAGAATCTTCAAAAACAATTTAGTGCCCTGGTTGGTTCATCACCCGTGGTTGAAAA GCAGGCCCCTTCAAGTTTTCAGTTCAACTGGACTGAAGAAGGCACTGATAGCCCTTGTTTCCATGGACAC AGCCTCCAGGGACTCCTGATAATGAAAGGCCAGTCCATGATAACCAAGAACTCCCTGTATTGGCTCAGTA CCCAGAAGTTCTGCAAAAGCTATTGTAAACATGTGACAACTTACGAAGAATCAAATTTTTCTTTGAGTTA CCAGTTTATACTAAACATCTTCTCCTTCTTACTGAGAATAAAGACCTCTGCTCTCCATGAAGAAGTGAGC TTACTTGAAAAGAAACTTTTTGAAAAAAAATACAACGAATCAAAAAAGAGCTCAGGATCCAAGAAAGGAA GAAGACAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209153 representing NM_020614
Red=Cloning site Green=Tags(s) MDVEEVKAFRDRCSQCAAVSWGLTDEGKYYCTSCHNVTDRSEEVVSAADIPNTKINSINRGLRQRSKHEK GWDWYVCEGFQCILYHQAKALETLGVSPELKNEVLHNFWKRYLQKSKQAYCKNPVRTSGRKAKVLEDSVQ SSDLGSDLELLSDTTCPLESEAEFQSDPQIPKPFPVTKGSPKSASVCSGSVDGVEYSERKEKGLVKMTVP RTLALCSLSLLWQRETITVSDLLRFVEEDHIPYINAFKLFPEEMKVYGRDKGIFAIESWPDYEDIYKNMI ELAIFLDLPRFPDITEDCYLHPNTLCMKYLLEVNLPDEMHTLTCQVVKLTGIGEVDFLTFDPIAKTKRTV KYDVQAMAVIVVVLKLLFLLDDKLEWSYSDLAEAYNEQHREDTPQFDFRKWYQVMKKTFDEKRRKWEEAR ARYAWKTKRPLYRSHIDKSVAYKRRKMVENLQKQFSALVGSSPVVEKQAPSSFQFNWTEEGTDSPCFHGH SLQGLLIMKGQSMITKNSLYWLSTQKFCKSYCKHVTTYEESNFSLSYQFILNIFSFLLRIKTSALHEEVS LLEKKLFEKKYNESKKSSGSKKGRRH TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020614 |
ORF Size | 1758 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020614.2, NP_065639.2 |
RefSeq Size | 2019 bp |
RefSeq ORF | 1761 bp |
Locus ID | 21340 |
UniProt ID | P97358 |
Cytogenetics | 12 A1.3 |
Gene Summary | Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219283 | Taf1b (untagged) - Mouse TATA box binding protein (Tbp)-associated factor, RNA polymerase I, B (Taf1b), transcript variant 1, (10ug) |
USD 601.00 |
|
MR209153 | Taf1b (Myc-DDK-tagged) - Mouse TATA box binding protein (Tbp)-associated factor, RNA polymerase I, B (Taf1b), transcript variant 1 |
USD 545.00 |
|
MR209153L3 | Lenti ORF clone of Taf1b (Myc-DDK-tagged) - Mouse TATA box binding protein (Tbp)-associated factor, RNA polymerase I, B (Taf1b), transcript variant 1 |
USD 845.00 |
|
MR209153L4 | Lenti ORF clone of Taf1b (mGFP-tagged) - Mouse TATA box binding protein (Tbp)-associated factor, RNA polymerase I, B (Taf1b), transcript variant 1 |
USD 845.00 |
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