Poll (NM_020032) Mouse Tagged ORF Clone
CAT#: MG209017
- TrueORF®
Poll (tGFP-tagged) - Mouse polymerase (DNA directed), lambda (Poll)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_020032" in other vectors (3)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Poll |
Synonyms | 1110003P06Rik; AV007317 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG209017 representing NM_020032
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCTCAGGGCATCGTGAAGGCCTTTCCCAAGCGGAAGAAAAGTCATGCAGATCTATCTTCAAAAG CACTTGCAAAGATTCCCAAAAGGGAGGTGGGAGAAGCTAGAGGATGGCTGAGCTCCCTGAGAGCCCACAT TATGCCCGCTGGCATTGGACGTGCCCGGGCTGAACTCTTTGAGAAGCAGATTATCCACCATGGCGGCCAG GTGTGCTCTGCCCAGGCCCCAGGAGTCACTCACATTGTGGTGGATGAAGACATGGACTATGAGCGGGCTC TCCGGCTCCTCAGGTTGCCCCAGCTACCCCCTGGTGCCCAGCTGGTGAAGTCCACCTGGCTGAGCTTGTG CCTGCAGGAGGGAAGGCTGACAGACACGGAAGGATTCAGCCTTCCCATGCCCAAGAGGTCCTTGGATGAA CCACAGCCCAGCAAGTCAGGCCAAGATGCTTCTGCTCCTGGCACCCAGCGGGATTTACCCAGGACCACCC TTTCTCTTTCCCCTCCTCACACCAGAGCTGTATCTCCTCCCCCAACGGCAGAAAAGCCATCAAGAACCCA AGCCCAGCTCAGCTCAGAGGATGAAACCAGCGATGGAGAAGGGCCCCAGGTTAGCTCAGCAGATCTGCAA GCCTTGATCACTGGGCACTACCCCACTCCCCCTGAGGAAGATGGTGGGCCTGACCCAGCACCAGAAGCTC TGGATAAATGGGTCTGTGCACAGCCCTCGAGCCAGAAGGCAACTAACTACAATCTGCACATCACAGAGAA GCTGGAAGTGCTGGCTAAAGCCTACAGTGTCCAGGGAGACAAGTGGAGGGCCCTGGGCTATGCAAAGGCC ATCAATGCTCTCAAGAGCTTCCACAAGCCTGTCAGCTCCTACCAGGAGGCCTGTAGCATCCCGGGAATTG GCAAGCGGATGGCGGAGAAGGTCATGGAGATCCTGGAGAGTGGGCATCTGCGGAAGCTAGACCACATCAG TGACAGTGTGCCTGTCTTGGAGCTCTTCTCCAACATCTGGGGAGCTGGGACGAAGACTGCCCAGATGTGG TACCATCAGGGCTTCCGAAACCTAGAAGATCTCCAAAGCCTGGGCTCCCTGACCGCTCAGCAGGCCATTG GCTTGAAGCACTACGATGACTTCCTGGACCGCATGCCCAGGGAGGAGGCTGCGGAAATTGAGCAGACGGT CCGGATATCAGCCCAGGCCTTCAACCCTGGGCTGCTGTGTGTGGCTTGTGGCTCTTACCGACGGGGGAAG ATGACGTGCGGGGATGTGGATGTACTCATTACTCACCCCGATGGCCGATCCCACCGGGGCATCTTCAGCT GCCTCCTTGATAGCCTTCGGCAGCAAGGGTTCCTCACAGATGACTTGGTGAGCCAGGAGGAGAACGGCCA ACAGCAGAAATACCTGGGTGTGTGCCGGCTCCCAGGGCCCGGGAAGCGCCACCGGCGACTGGACATCATC GTGGTACCCTACTGTGAGTTTGCCTGTGCCCTGCTCTACTTCACCGGCTCTGCCCACTTCAACCGGTCCA TGAGAGCTCTGGCCAAGACCAAGGGCATGAGCCTGTCGGAGCATGCGCTCAGTGCAGCTGTAGTCCGGAA CAGCCAAGGTGTCAAGGTGGGGCCTGGACAAGTGCTGCCCACCCCCACGGAGAAGGACGTCTTCAAGCAC TTAGGCCTGCCCTACCGGGAGCCAGCTGAACGGGACTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209017 representing NM_020032
Red=Cloning site Green=Tags(s) MDPQGIVKAFPKRKKSHADLSSKALAKIPKREVGEARGWLSSLRAHIMPAGIGRARAELFEKQIIHHGGQ VCSAQAPGVTHIVVDEDMDYERALRLLRLPQLPPGAQLVKSTWLSLCLQEGRLTDTEGFSLPMPKRSLDE PQPSKSGQDASAPGTQRDLPRTTLSLSPPHTRAVSPPPTAEKPSRTQAQLSSEDETSDGEGPQVSSADLQ ALITGHYPTPPEEDGGPDPAPEALDKWVCAQPSSQKATNYNLHITEKLEVLAKAYSVQGDKWRALGYAKA INALKSFHKPVSSYQEACSIPGIGKRMAEKVMEILESGHLRKLDHISDSVPVLELFSNIWGAGTKTAQMW YHQGFRNLEDLQSLGSLTAQQAIGLKHYDDFLDRMPREEAAEIEQTVRISAQAFNPGLLCVACGSYRRGK MTCGDVDVLITHPDGRSHRGIFSCLLDSLRQQGFLTDDLVSQEENGQQQKYLGVCRLPGPGKRHRRLDII VVPYCEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSAAVVRNSQGVKVGPGQVLPTPTEKDVFKH LGLPYREPAERDW TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020032 |
ORF Size | 1719 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020032.3 |
RefSeq Size | 2336 bp |
RefSeq ORF | 1722 bp |
Locus ID | 56626 |
UniProt ID | Q9QXE2 |
Cytogenetics | 19 C3 |
Gene Summary | DNA polymerase that functions in several pathways of DNA repair. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination. Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities. Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR209017 | Poll (Myc-DDK-tagged) - Mouse polymerase (DNA directed), lambda (Poll) |
USD 534.00 |
|
MR209017L3 | Lenti ORF clone of Poll (Myc-DDK-tagged) - Mouse polymerase (DNA directed), lambda (Poll) |
USD 834.00 |
|
MR209017L4 | Lenti ORF clone of Poll (mGFP-tagged) - Mouse polymerase (DNA directed), lambda (Poll) |
USD 834.00 |
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