Nt5c2 (NM_029810) Mouse Tagged ORF Clone
CAT#: MG208873
- TrueORF®
Nt5c2 (tGFP-tagged) - Mouse 5'-nucleotidase, cytosolic II (Nt5c2)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_029810" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Nt5c2 |
Synonyms | 2010002I23Rik; cN-II; CnII; ENSMUSG00000025039; Gm9751; Gmp; Nt5b; Pnt5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG208873 representing NM_029810
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGACCTCCTGGAGTGACCGCTTACAGAATGCAGCAGATGTGCCTGCTAACATGGATAAGCATGCCT TGAAAAAGTACCGTCGAGAAGCCTATCACCGGGTGTTTGTGAACCGAAGTTTAGCCATGGAAAAAATAAA GTGTTTTGGTTTTGATATGGATTATACCCTAGCCGTGTACAAGTCTCCAGAATATGAATCCCTTGGCTTT GAGCTTACTGTGGAGAGATTAGTTTCCATTGGCTATCCTCAGGAGCTGCTCAGCTTTGCATATGATTCTA CATTCCCTACCAGAGGACTTGTCTTTGACACGCTCTATGGAAATCTTTTGAAAGTCGATGCCTATGGAAA CCTCTTGGTGTGTGCACATGGATTTAACTTCATAAGAGGACCAGAAACTAGAGAGCAGTATCCAAATAAA TTTATCCAACGAGATGACACTGAACGGTTTTACATTCTGAACACACTATTCAATCTCCCAGAGACCTACC TGTTGGCCTGCCTCGTAGATTTTTTTACTAATTGTCCCAGATACACCAGTTGTGACACAGGATTTAAAGA TGGGGACCTCTTCATGTCCTACCGTAGCATGTTCCAGGACGTAAGAGACGCAGTGGACTGGGTCCATTAC AAGGGCTCCCTTAAGGAGAAGACGGTTGAAAATCTTGAGAAGTATGTCGTCAAGGATGGCAAGTTGCCGT TGCTTCTGAGCCGAATGAAGGAAGTAGGGAAAGTGTTTCTGGCTACTAACAGTGACTATAAGTACACAGA TAAAATCATGACTTACCTGTTTGATTTCCCGCATGGCCCCAAGCCCGGGAGCTCCCACCGGCCGTGGCAG TCCTACTTTGACCTGATTTTGGTGGATGCTCGGAAACCACTCTTTTTTGGAGAAGGCACAGTGCTGCGTC AGGTGGACACTAAAACTGGAAAACTGAAAATTGGCACCTACACGGGCCCCCTGCAGCACGGCATTGTCTA CTCAGGGGGTTCATCTGATACAATCTGTGACCTGTTGGGAGCTAAGGGCAAAGACATTTTGTATATTGGA GATCACATTTTTGGAGACATTCTGAAGTCAAAGAAACGGCAAGGGTGGAGGACTTTCTTGGTGATTCCTG AGCTTGCGCAGGAGCTCCACGTCTGGACGGACAAGAGTTCACTCTTTGAAGAACTTCAAAGCTTGGATAT TTTCTTGGCTGAACTCTACAAGCACCTGGACAGCAGTAGCAATGAGCGCCCGGACATTAGTTCCATCCAG AGACGGATTAAGAAAGTAACTCATGACATGGACATGTGCTATGGGATGATGGGAAGCCTGTTCCGCAGCG GCTCCCGGCAGACACTCTTTGCCAGCCAAGTGATGCGCTATGCAGATCTCTATGCGGCCTCGTTCATCAA CCTGCTGTACTACCCATTCAGCTACCTCTTCAGAGCTGCCCATGTCCTGATGCCGCACGAGTCAACGGTG GAACACACGCATGTGGATATCAATGAGATGGAGTCCCCTCTCGCCACCCGGAACCGCACGTCAGTGGATT TCAAAGACACTGACTATAAGCGACACCAGCTTACAAGGTCCATTAGTGAGGTCAAGCCCCCCGACCTCTT CCCGCTGGCCCCCCAGGAGATCACACACTGCCATGATGAAGATGATGATGAAGAGGAAGAAGAGGAAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208873 representing NM_029810
Red=Cloning site Green=Tags(s) MTTSWSDRLQNAADVPANMDKHALKKYRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGF ELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNK FIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCDTGFKDGDLFMSYRSMFQDVRDAVDWVHY KGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQ SYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIG DHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQ RRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTV EHTHVDINEMESPLATRNRTSVDFKDTDYKRHQLTRSISEVKPPDLFPLAPQEITHCHDEDDDEEEEEEE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_029810 |
ORF Size | 1680 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_029810.2, NP_084086.2 |
RefSeq Size | 2729 bp |
RefSeq ORF | 1683 bp |
Locus ID | 76952 |
UniProt ID | Q3V1L4 |
Cytogenetics | 19 C3 |
Gene Summary | May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202737 | Nt5c2 (untagged) - Mouse 5'-nucleotidase, cytosolic II (Nt5c2), transcript variant 3, (10ug) |
USD 522.00 |
|
MR208873 | Nt5c2 (Myc-DDK-tagged) - Mouse 5'-nucleotidase, cytosolic II (Nt5c2), transcript variant 3 |
USD 522.00 |
|
MR208873L3 | Lenti ORF clone of Nt5c2 (Myc-DDK-tagged) - Mouse 5'-nucleotidase, cytosolic II (Nt5c2), transcript variant 3 |
USD 822.00 |
|
MR208873L4 | Lenti ORF clone of Nt5c2 (mGFP-tagged) - Mouse 5'-nucleotidase, cytosolic II (Nt5c2), transcript variant 3 |
USD 822.00 |
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