Adgrg3 (NM_173036) Mouse Tagged ORF Clone

CAT#: MG208657

Gpr97 (tGFP-tagged) - Mouse G protein-coupled receptor 97 (Gpr97)

Product Images

Circular map for MG208657

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adgrg3
• Synonyms: A030001G24Rik; AW214383; Gpr97; Pb1; Pb99
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208657 representing NM_173036
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACAGCCAGGAGTCTGGGCCTCCTGTTCTTTCTGCTGCTGACTTCAGATGAGGAGACCACGGAAG
  AGCCGAGGAATGTCTGCCGTCGGCTTCAGGAGGGCCACGAGTATGACACCTTCGACTTGAATGACACTGC
  TCAGTGTTTCACCAAGTGTGGGCAATCGGAGCACAGCCCCTGTGACGTGGGGAACCTGCAGAGATACTGG
  TTGAACTATGAGTCCTACCTGCTGGAGAATTCCATGGAGACTGTGGACATGCCTTTTGTTAAGGCATTGA
  TCCAGAACATCAGCACGGATGTCTCAGAAGACCTGCTGTACTCTCTGATGCTTTCCCAGATCCCGAGGCA
  GGTGATGCAGGGTGAGGACGAGCCTGCTGATGGAGTCCGACTTCCCAAGAGCCTGTTTGGAGCCCTGCCT
  GGCAACAGGTCTGCAGTCCGGTTGGCCATAACTGTTCTAGACATTGGTGCAGGGAATGTCTTTAAGGGCC
  CCAAGCTCCTTGAGGACAAGGGCAGCAGCGTGTTGAACAACCGCATGGTGGGTTTGAGCGTGGGCCAGAT
  GCACGCCACCGGGCTGTCGGAACCTGTAGAGATCACCTTCTCCCATGAGCGTCAACCACCTAACGTGATC
  CTCACCTGTGTATTCTGGGATATGGCTAAAGGAGACTGGGATTCCCACGGCTGCTCCACGGTGCCCGGGG
  ATGGGAGAACTGTCTGCCGTTGCGACCACTTGACCTTCTTCGCCTTGCTGCTGAGGCCGATCTTGGACCT
  GGCCACGGCACAGACTCTCACTCGCATCTCCCAGGCAGGCAGTGCCGTCTCCATGATCTTCCTGGCCTTC
  ACCATGGTGCTCTATGTTGCCTTCAGGTTCTCTCTGCAGAGGCTCAAGTCTGAAGATGCCCCTAAGATCC
  ACATGGCTCTGAGCATCAGCCTGTTTCTCCTGAATCTTACCTTCCTGATCAATGTGGGGAGCAGCTCCCA
  AGGCCCCCCAGCCTCCTGCTGGGTCCGAGCTGCCATTTTCCACTACTTTCTGCTCTGTGTCTTCACCTGG
  ATGGGTCTGGAGGCCTTCCACCTCTACCTGCTGGCCATCAGAGTCTTCAATACCTACTTTGGACACTATT
  TCCTGAAGCTGAGCCTGCTGGCCTGGGGCCTGCCTGTTCTTGTAGTTATTGGTGCTGGAAGTAGCAACAG
  TTATGGAGTTTACACCATTCGCGACCAGGAGAACCGCACATCACTGGAGTTGTGCTGGTTCCAGAAAGAG
  CCTGCTCTTTATGCTACTGTCCACGGCTACTTCCTTGTTACCTTCCTCTTCGGTGCTGTGGTACTGGCTT
  TGGTGGCCTGGAAGATCTTCACTCTGCCCAGTGTCACAGCGGGCAAAGGACAGGGGCCGACCTGGAAGTC
  GGTCCTCACCGTGCTGGGCCTCTCCAGTCTGGTGGGCATGACCTGGGGGCTGGCTGTTCTCACCCCCCTC
  GGCTTGTCTACCATCTACGTCTTCACCCTCTTAAACTCTCTACAAGGGCTCTTCATCTTCTGCTGGTTCA
  TCATCCTCTACTTCCCTACTCAGAGCACCACAGCCTCCTCCTCCGGCACCGCCCGCCTGGACCAGGCCCA
  CTCCGTGTCCCAGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208657 representing NM_173036
  Red=Cloning site Green=Tags(s)
  
  MATARSLGLLFFLLLTSDEETTEEPRNVCRRLQEGHEYDTFDLNDTAQCFTKCGQSEHSPCDVGNLQRYW
  LNYESYLLENSMETVDMPFVKALIQNISTDVSEDLLYSLMLSQIPRQVMQGEDEPADGVRLPKSLFGALP
  GNRSAVRLAITVLDIGAGNVFKGPKLLEDKGSSVLNNRMVGLSVGQMHATGLSEPVEITFSHERQPPNVI
  LTCVFWDMAKGDWDSHGCSTVPGDGRTVCRCDHLTFFALLLRPILDLATAQTLTRISQAGSAVSMIFLAF
  TMVLYVAFRFSLQRLKSEDAPKIHMALSISLFLLNLTFLINVGSSSQGPPASCWVRAAIFHYFLLCVFTW
  MGLEAFHLYLLAIRVFNTYFGHYFLKLSLLAWGLPVLVVIGAGSSNSYGVYTIRDQENRTSLELCWFQKE
  PALYATVHGYFLVTFLFGAVVLALVAWKIFTLPSVTAGKGQGPTWKSVLTVLGLSSLVGMTWGLAVLTPL
  GLSTIYVFTLLNSLQGLFIFCWFIILYFPTQSTTASSSGTARLDQAHSVSQE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173036
• ORF Size: 1626 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173036.2, NP_766624.2
• RefSeq Size: 2433 bp
• RefSeq ORF: 1629 bp
• Locus ID: 54672
• UniProt ID: Q8R0T6
• Cytogenetics: 8 C5
• Gene Summary: Orphan receptor that regulates migration of lymphatic endothelial cells via the small GTPases RhoA and CDC42 (By similarity). Regulates B-cell development (PubMed:24113187). Seems to signal through G-alpha(q)-proteins (By similarity).[UniProtKB/Swiss-Prot Function]