Alg8 (NM_199035) Mouse Tagged ORF Clone

CAT#: MG208441

Alg8 (tGFP-tagged) - Mouse asparagine-linked glycosylation 8 homolog (yeast, alpha-1,3-glucosyltransferase) (Alg8)

Product Images

Circular map for MG208441

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Alg8
• Synonyms: AI447372; Gm1089
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208441 representing NM_199035
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGTCCGGGTCTGCAACCGCTGGTGGCCATTGGTTCTCTGCTTTGGCTCTTGGGGTGACTCTCC
  TTAAGTGCCTTCTCATCCCCACCTACCATTCCACGGATTTTGAAGTACACCGAAATTGGCTTGCAATCAC
  TCACAGCCTCCCAATATCTCAGTGGTATTATGAGGCAACTTCAGAGTGGACCTTGGATTACCCCCCCTTT
  TTTGCATGGTTTGAGTACGCCTTATCACATATTGCCAAATACTTTGATCAAGAGATGCTCAATATTCATA
  ACCTGAACTATTACAGTTCAAGGACATTGCTTTTCCAGAGGTTTTCTGTCATCTTGACAGATGCACTCTT
  TGTGTATGCTGTCCATGAGTGCTGTAAATGCATTGATGGAAAAAGAACAGGCAAAGACCTCACAGAGAAA
  CCAAAGTTTATTCTGTCCGTGCTCCTGCTGTGGAACTTTGGGTTGTTAATTGTAGACCATATTCATTTTC
  AGTACAATGGCTTTCTGTCTGGATTACTGCTGTTGTCCATTGCACGTCTATTTCAGAAAAGGCACATTGA
  AGGGGCGTTGCTCTTTGCTGTCCTCCTCCATCTCAAGCACATCTACCTATATGTGGCACCAGCCTATGGT
  GTCTATCTGCTGCGGTCCTACTGCTTCACTGCAAGTAAACCAGATGGCAGTGTCCGTTGGAGCAGCTTCA
  GCGTTGTCCGCGTTACTTCCCTGGGGCTGATCGTGTTCCTAGTTTCTGCTCTTTCGTTGGGCCCTTTCCT
  AGCCTTGAACCAGCTGCCACAAGTCTTTTCCCGACTCTTTCCTTTCAAGAGGGGCCTTTGCCATGCCTAC
  TGGGCTCCAAACTTCTGGGCTTTGTACAATGCTTTGGACAAAGTACTGTCTGTAATTGGTTTGAAATTGA
  AGCTTCTTGACCCCAGCCAGATCCCCAGGGCCTCAATGACAAGTGGTTTGGTTCAGCAGTTCCAGCACAC
  AGTCCTTCCCTCAGTTTCCCCTTTGGCAACCCTCATCTGCACACTGATAGCCATATTGCCCTCTGTTTTC
  TGTCTTTGGTTTAAACCCCAAGGGCCCAGAGGCTTTCTCCGATGCCTGGTTCTTTGTGCCTTGAGTTCCT
  TCATGTTTGGGTGGCATGTCCATGAGAAAGCCATACTCCTTGCGATTCTCCCAATGAGCCTGTTGTCTGT
  GGAAAAAGCAGGAGATGCGACAGTTTTTCTGATTCTGGCGACAACAGGACACTATTCCCTCTTCCCCCTG
  CTCTTCACTGCACCAGAACTTCCCATTAAAATCTTACTCATGTTACTGTTCACGGTTTATAGTATTTCCT
  CACTGAAGACACTATTCAGAAAAGAAAAACCTCTTTTTAATTGGATGGAAACTGTGTACCTCCTTGGCCT
  GGGGCCTCTGGAAGTCTGCTGTGAATTCCTACTCCCGTTTACCTCCTGGAAGCTGAAGTACCCCTTCATC
  CCTTTGCTGCTGACCTCGGTGTACTGTGCAGTGGGCATCACATACGCCTGGACCAGGCTGTATGCTTCAG
  TGTTGACTGGCTCCCTTGTCAGCAAGACAAAGAAACAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208441 representing NM_199035
  Red=Cloning site Green=Tags(s)
  
  MAASGSATAGGHWFSALALGVTLLKCLLIPTYHSTDFEVHRNWLAITHSLPISQWYYEATSEWTLDYPPF
  FAWFEYALSHIAKYFDQEMLNIHNLNYYSSRTLLFQRFSVILTDALFVYAVHECCKCIDGKRTGKDLTEK
  PKFILSVLLLWNFGLLIVDHIHFQYNGFLSGLLLLSIARLFQKRHIEGALLFAVLLHLKHIYLYVAPAYG
  VYLLRSYCFTASKPDGSVRWSSFSVVRVTSLGLIVFLVSALSLGPFLALNQLPQVFSRLFPFKRGLCHAY
  WAPNFWALYNALDKVLSVIGLKLKLLDPSQIPRASMTSGLVQQFQHTVLPSVSPLATLICTLIAILPSVF
  CLWFKPQGPRGFLRCLVLCALSSFMFGWHVHEKAILLAILPMSLLSVEKAGDATVFLILATTGHYSLFPL
  LFTAPELPIKILLMLLFTVYSISSLKTLFRKEKPLFNWMETVYLLGLGPLEVCCEFLLPFTSWKLKYPFI
  PLLLTSVYCAVGITYAWTRLYASVLTGSLVSKTKKH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199035
• ORF Size: 1578 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199035.2, NP_950200.2
• RefSeq Size: 2002 bp
• RefSeq ORF: 1581 bp
• Locus ID: 381903
• UniProt ID: Q6P8H8
• Cytogenetics: 7 E1
• Gene Summary: Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(1)Man(9)GlcNAc(2)-PP-Dol before it is transferred to the nascent peptide (By similarity). Required for PKD1/Polycystin-1 maturation and localization to the plasma membrane of the primary cilia (PubMed:28375157).[UniProtKB/Swiss-Prot Function]