Arsg (NM_028710) Mouse Tagged ORF Clone

CAT#: MG208429

Arsg (tGFP-tagged) - Mouse arylsulfatase G (Arsg)

Product Images

Circular map for MG208429

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arsg
• Synonyms: 6330406P08Rik; AI846872
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208429 representing NM_028710
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGGCTCTTTCTAAAGGTTTTGTTGGTTGGCATGGCTTTTTCTGGATTTTTCTACCCTCTCGTGG
  ATTTCTCTATCAGTGGGAAAACAAGAGCCCCCCAACCAAACATCGTGATCATTTTGGCTGATGACATGGG
  ATGGGGTGATCTGGGAGCAAACTGGGCAGAAACAAAGGACACCACCAATCTTGACAAGATGGCTTCCGAA
  GGAATGAGGTTTGTAGACTTCCACGCAGCTGCTTCTACCTGCTCCCCATCCCGAGCCTCCCTGCTCACTG
  GCCGGCTGGGTCTACGGAACGGAGTCACGCACAACTTCGCAGTCACCTCTGTAGGGGGGCTTCCAGTCAA
  CGAGACCACCTTGGTAGAGGTGCTGCGACAGGAGGGTTACGTCACTGCCATGATAGGCAAGTGGCATCTT
  GGGCATCATGGCTCTTATCATCCCAATTTCCGTGGCTTCGATTACTACTTTGGAATCCCATATAGCAATG
  ACATGGGTTGCACTGATGCTCCAGGCTACAACTACCCTCCTTGTCCAGCGTGTCCACAGAGAGATGGCCT
  ATGGAGGAACCCTGGGAGGGACTGTTACACAGATGTTGCCCTTCCTCTCTATGAAAACCTGAACATCGTG
  GAGCAGCCTGTGAACCTGAGCGGCCTTGCACAGAAGTACGCAGAAAGGGCAGTGGAGTTCATTGAGCAGG
  CAAGCACCAGTGGAAGACCCTTCCTGCTCTACGTGGGCCTGGCTCACATGCATGTGCCTTTGTCTGTAAC
  TCCACCATTGGCACACCCACAGCGCCAAAGCCTGTACCGTGCAAGTCTCCGGGAGATGGACAGTCTGGTA
  GGGCAGATCAAGGACAAAGTTGACCATGTGGCAAGAGAAAACACACTCCTCTGGTTTACAGGAGACAATG
  GCCCATGGGCTCAGAAGTGTGAGCTGGCAGGCAGCGTGGGTCCCTTCTTCGGGTTGTGGCAAACCCATCA
  AGGGGGAAGTCCAACCAAGCAGACCACCTGGGAAGGTGGGCATCGGGTTCCAGCACTGGCTTACTGGCCT
  GGCAGAGTTCCAGCCAACGTCACTAGCACCGCCTTGTTAAGCTTGCTAGACATCTTCCCCACCGTGATAG
  CTCTGGCAGGAGCCAGCTTGCCCCCAAACCGGAAATTTGATGGACGGGATGTCTCTGAGGTGCTCTTTGG
  CAAGTCACAGATGGGGCACAGGGTGCTATTCCACCCCAACAGTGGGGCTGCTGGAGAGTATGGCGCCCTG
  CAGACTGTCCGCCTAAACCATTACAAAGCTTTCTACATCACTGGTGGAGCCAAAGCTTGTGATGGGAGCG
  TGGGTCCTGAGCAGCACCACGTGGCCCCTCTCATTTTCAACCTAGAAGATGCTGCTGATGAAGGGATGCC
  TCTACAGAAGGGGAGCCCCGAGTACCAGGAGGTGCTGCAGCAGGTCACCAGGGCTCTCGCAGATGTCCTC
  CAAGACATCGCTGATGACAACAGCTCCCGAGCAGACTACACTCAGGATCCTTCAGTGATTCCTTGCTGTA
  ACCCCTATCAAACTACCTGCCGTTGCCAACCGGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208429 representing NM_028710
  Red=Cloning site Green=Tags(s)
  
  MGWLFLKVLLVGMAFSGFFYPLVDFSISGKTRAPQPNIVIILADDMGWGDLGANWAETKDTTNLDKMASE
  GMRFVDFHAAASTCSPSRASLLTGRLGLRNGVTHNFAVTSVGGLPVNETTLVEVLRQEGYVTAMIGKWHL
  GHHGSYHPNFRGFDYYFGIPYSNDMGCTDAPGYNYPPCPACPQRDGLWRNPGRDCYTDVALPLYENLNIV
  EQPVNLSGLAQKYAERAVEFIEQASTSGRPFLLYVGLAHMHVPLSVTPPLAHPQRQSLYRASLREMDSLV
  GQIKDKVDHVARENTLLWFTGDNGPWAQKCELAGSVGPFFGLWQTHQGGSPTKQTTWEGGHRVPALAYWP
  GRVPANVTSTALLSLLDIFPTVIALAGASLPPNRKFDGRDVSEVLFGKSQMGHRVLFHPNSGAAGEYGAL
  QTVRLNHYKAFYITGGAKACDGSVGPEQHHVAPLIFNLEDAADEGMPLQKGSPEYQEVLQQVTRALADVL
  QDIADDNSSRADYTQDPSVIPCCNPYQTTCRCQPV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028710
• ORF Size: 1575 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028710.2, NP_082986.2
• RefSeq Size: 2982 bp
• RefSeq ORF: 1578 bp
• Locus ID: 74008
• UniProt ID: Q3TYD4
• Cytogenetics: 11 E1
• Gene Summary: Displays arylsulfatase activity with pseudosubstrates at acidic pH, such as p-nitrocatechol sulfate.[UniProtKB/Swiss-Prot Function]