Nsun2 (BC013625) Mouse Tagged ORF Clone

CAT#: MG208356

Nsun2 (tGFP-tagged) - Mouse NOL1/NOP2/Sun domain family 2 (cDNA clone MGC:18985 IMAGE:4011674)

Product Images

Circular map for MG208356

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nsun2
• Synonyms: D13Wsu123e; Misu
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208356 representing BC013625
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGTAAACCATGACGCATCCAGCATACCTAGACTTACAGTAGATGTGGACGGAAGGAAAGAGATTC
  TCTTCTATGATCGAATTTTATGTGATGTCCCTTGCAGTGGCGATGGCACAATGAGAAAAAACATTGATGT
  CTGGAAGAAATGGACAACCTTAAACAGCTTGCAGCTCCATGGCCTGCAGCTTCGGATTGCAACTCGAGGT
  GCTGAGCAGCTGGCGGAAGGTGGCAGGATGGTGTATTCCACGTGTTCCTTGAACCCCGTGGAGGATGAAG
  CAGTGATCGCAGCTCTGCTAGAGAAGAGTGAAGGAGCTCTTGAGCTTGCTGATGTGTCTGCTGAGTTGCC
  AGGACTGAAGTGGATGCCTGGAGTCTCACAGTGGAAGGTCATGACTAGAGACGGGCAGTGGTTTGCAGAC
  TGGCATGAGGTTCCCCAGGGCAGGCATACACAAATCCGACCTACCATGTTCCCACCAACGGACCTGGAGA
  AGCTACAGGCAATGCATCTAGAGCGATGCCTTCGAATCCTGCCCCATCATCAGAATACTGGAGGGTTCTT
  TGTGGCAGTATTGGTCAAGAAAGCACCAATGCCGTGGAACAAACGTCAGCCCAAGGTCCAGAATAAATCT
  GCAGAAGCCAGAGAACCCAGGGTATCCAGCCATGTGGCTGCCACAGAGGGAAATCCCAGTGACCAGTCTG
  AGCTGGAAAGTCAGATGATAACTGGAGCTGGTGACTCAGAAACAGCTCACAACACTGAGAACACAGAGAG
  CAATGAGAAGAAAGATGGCGTGTGTGGCCCTCCTCCATCAAAGAAAATGAAGTTGTTTGGATTTAAAGAA
  GATCCATTTGTATTCATTCCTGAAGATGATCCTTTATTTCCACCTATTGAGAAGTTTTATGCCTTGGATC
  CTTCATTCCCGAGGATGAATCTGTTAACCCGAACCACAGAAGGAAAGAAGCGGCAGCTTTATATGGTCTC
  CAAGGAGCTGAGGAATGTACTGCTGAACAACAGCGAGAAGATGAAGGTCATTAACACTGGGATAAAAGTC
  TGGTGTCGAAATAACAGTGGTGAAGAATTCGATTGTGCATTCCGTTTGGCACAGGAGGGAATATATACAT
  TGTATCCATTTATCAATTCAAGAATCATCACTGTATCAATGGAAGACGTGAAGACACTGTTGACCCAGGA
  GAACCCATTCTTTAGAAAACTGAGCAGTGAGGCCTACAGTCAAGTCAAGGACCTCGCAAAGGGAAGTGTT
  GTGCTGAAGTATGAGCCAGATTCTGCGAATCCAGACACCCTCCAGTGCCCCATCGTGCTGTGTGGGTGGC
  GGGGAAAGGCCTCTATTCGAACTTTTGTGCCCAAAAATGAGCGGCTTCATTATCTCAGGATGATGGGTCT
  GGAGGTACTGGGAGAAAAGAAGAAAGAGGGAGTCATTCTTACCAATGAGAATGCTGCCAGCCCAGAGCAG
  CCTGGAGATGAGGATGCCAAGCAGACAGCACAAGACCCCTGCGTCCCAGACTCCGTCCCTGGCTGTGATG
  CAGCTGCAGCTGAGCCATCCCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208356 representing BC013625
  Red=Cloning site Green=Tags(s)
  
  MVVNHDASSIPRLTVDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRG
  AEQLAEGGRMVYSTCSLNPVEDEAVIAALLEKSEGALELADVSAELPGLKWMPGVSQWKVMTRDGQWFAD
  WHEVPQGRHTQIRPTMFPPTDLEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKAPMPWNKRQPKVQNKS
  AEAREPRVSSHVAATEGNPSDQSELESQMITGAGDSETAHNTENTESNEKKDGVCGPPPSKKMKLFGFKE
  DPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKV
  WCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKTLLTQENPFFRKLSSEAYSQVKDLAKGSV
  VLKYEPDSANPDTLQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNENAASPEQ
  PGDEDAKQTAQDPCVPDSVPGCDAAAAEPSR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC013625
• ORF Size: 1565 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013625, AAH13625
• RefSeq Size: 2325 bp
• RefSeq ORF: 1565 bp
• Locus ID: 28114
• Cytogenetics: 13 35.55 cM
• Gene Summary: RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors (By similarity). May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.[UniProtKB/Swiss-Prot Function]