Uap1 (BC017547) Mouse Tagged ORF Clone
CAT#: MG208347
- TrueORF®
Uap1 (tGFP-tagged) - Mouse UDP-N-acetylglucosamine pyrophosphorylase 1 (cDNA clone MGC:28907 IMAGE:4920702)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"BC017547" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Uap1 |
Synonyms | AGX-1, AGX-2, AgX, AGX1, SPAG2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG208347 representing BC017547
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGTCAATGACCTCAAACAAAGGCTGTCCCAAGCTGGGCAAGAGCACCTGCTGCAGTTCTGGAATG AGCTGTCCGAAGCCCAGCAGGTAGAACTTTATATGGAGCTCCAGGCCATGAACTTTGAAGAGCTGAACTC CTTTTTCCGGAAGGCCATTGGTGAATTTGATCGATCCTCTCACCAAGAAAAGGTGGACGCACGAATGGAG CCTGTGCCCCGGCAGGTTTTGGGCAGTGCTACCAGAGATCAAGAGCAGCTGCAGGCCTGGGAAAGTGAAG GACTTTCTCAGATTTCTCAAAACAAAGTAGCAGTTCTTCTTCTAGCTGGTGGGCAGGGGACAAGACTTGG AGTCTCATATCCCAAGGGCATGTATGATGTTGGCTTGCCATCCCACAAGACGCTTTTTCAGATTCAAGCA GAACGTATTCTGAAACTCCAGCAGTTAGCTGAAAAACACCATGGCAACAAGTGCACCATCCCATGGTATA TAATGACCAGCGGCAGAACAATGGAATCAACGAAGGAGTTCTTCACAAAGCACAAGTTCTTTGGTTTAAA AAAGGAGAACGTGGTGTTTTTCCAGCAGGGAATGCTGCCTGCTATGAGTTTTGATGGGAAAATTATCTTG GAAGAGAAAAACAAAGTTTCTATGGCTCCAGATGGGAATGGTGGTCTCTACCGGGCTCTTGCAGCCCAAA ACATTGTGGAGGACATGGAGCAAAGAGGCATCTGCAGTATTCATGTCTATTGTGTGGACAACATATTGGT AAAAGTGGCGGACCCACGGTTCATTGGATTCTGCATTCAGAAAGGAGCAGACTGTGGGGCAAAGGTGGTA GAGAAAACGAATCCCACAGAGCCAGTTGGAGTGGTTTGCCGGGTGGATGGAGTATACCAGGTGGTAGAAT ACAGCGAGATCTCTCTGGCAACAGCTCAAAGGCGAAGTTCAGATGGACGGCTGCTGTTCAATGCAGGGAA CATTGCCAATCACTTCTTCACTGTCCCGTTCCTGAAAGATGTTGTCAATGTTTATGAACCTCAGTTGCAG CACCATGTGGCTCAAAAGAAGATTCCATATGTGGACTCCCAGGGGTACTTCATTAAGCCAGACAAGCCAA ATGGAATAAAGATGGAAAAATTTGTCTTTGACATCTTCCAGTTTGCAAAGAAGTTTGTGGTGTATGAAGT ATTACGGGAAGATGAGTTTTCCCCGCTCAAGAATGCAGACAGTCAGAACGGGAAGGACAACCCGACCACG GCAAGGCATGCTTTGATGTCCCTTCACCACTGCTGGGTCCTCAATGCCGGGGGACACTTCATAGATGAGA ATGGCTCTCGCCTCCCAGCCATCCCCCGTGCTACAAATGGAAAGTCAGAGGCCATCACAGCTGATGTCAA TCACAACTTGAAAGATGCCAATGATGTACCGATCCAGTGTGAAATCTCTCCCCTCATCTCTTATGCTGGA GAGGGTCTTGAAGGTTATGTGGCAGATAAAGAGTTCCATGCACCGTTAATCATCGATGAGAATGGAGTTC ATGAGCTGGTGAAAAATGGCATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208347 representing BC017547
Red=Cloning site Green=Tags(s) MNVNDLKQRLSQAGQEHLLQFWNELSEAQQVELYMELQAMNFEELNSFFRKAIGEFDRSSHQEKVDARME PVPRQVLGSATRDQEQLQAWESEGLSQISQNKVAVLLLAGGQGTRLGVSYPKGMYDVGLPSHKTLFQIQA ERILKLQQLAEKHHGNKCTIPWYIMTSGRTMESTKEFFTKHKFFGLKKENVVFFQQGMLPAMSFDGKIIL EEKNKVSMAPDGNGGLYRALAAQNIVEDMEQRGICSIHVYCVDNILVKVADPRFIGFCIQKGADCGAKVV EKTNPTEPVGVVCRVDGVYQVVEYSEISLATAQRRSSDGRLLFNAGNIANHFFTVPFLKDVVNVYEPQLQ HHVAQKKIPYVDSQGYFIKPDKPNGIKMEKFVFDIFQFAKKFVVYEVLREDEFSPLKNADSQNGKDNPTT ARHALMSLHHCWVLNAGGHFIDENGSRLPAIPRATNGKSEAITADVNHNLKDANDVPIQCEISPLISYAG EGLEGYVADKEFHAPLIIDENGVHELVKNGI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC017547 |
ORF Size | 1565 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC017547, AAH17547 |
RefSeq Size | 2139 bp |
RefSeq ORF | 1565 bp |
Locus ID | 107652 |
Cytogenetics | 1 H3 |
Gene Summary | Converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC206525 | Uap1 (untagged) - Mouse UDP-N-acetylglucosamine pyrophosphorylase 1 (cDNA clone MGC:28907 IMAGE:4920702), (10ug) |
USD 486.00 |
|
MR208347 | Uap1 (Myc-DDK-tagged) - Mouse UDP-N-acetylglucosamine pyrophosphorylase 1 (cDNA clone MGC:28907 IMAGE:4920702) |
USD 485.00 |
|
MR208347L3 | Lenti ORF clone of Uap1 (Myc-DDK-tagged) - Mouse UDP-N-acetylglucosamine pyrophosphorylase 1 (cDNA clone MGC:28907 IMAGE:4920702) |
USD 785.00 |
|
MR208347L4 | Lenti ORF clone of Uap1 (mGFP-tagged) - Mouse UDP-N-acetylglucosamine pyrophosphorylase 1 (cDNA clone MGC:28907 IMAGE:4920702) |
USD 785.00 |
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