Dpys (NM_022722) Mouse Tagged ORF Clone

CAT#: MG208311

Dpys (tGFP-tagged) - Mouse dihydropyrimidinase (Dpys)

Product Images

Circular map for MG208311

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dpys
• Synonyms: 1200017I10Rik; 1300004I01Rik; DHP; DHPase
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208311 representing NM_022722
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCACAGGGACGACTTCTTATCCGCGGGGGTCTCATTGTCAATGATGACTTCTCACAGGTGGCCG
  ACGTGCTAGTGGAGGACGGCGTGGTGCGGGCGCTGGGACGGGACCTGCTGCCTCCTGAGGACGCATCTCG
  GGGGCTGCGGATCCTAGATGCAGCGGGCAAGCTCGTCCTGCCCGGGGGCATCGACACGCACACGCACATG
  CAATTCCCGTTCATGGGCTCGCAGTCAGTGGACGACTTCTACCAGGGCACCAAGGCTGCTTTGGCAGGAG
  GCACCACCATGATCATTGATTTTGCCATTCCTCAGAAAGGCAGCTCCCTCATCGAGGCCTTTGAGACTTG
  GCGCAACTGGGCAGACCCGAAAGTCTGCTGTGACTATAGCCTGCATGTGGCAGTGACGTGGTGGAGCGAC
  AAGGTAAAAGAAGAAATGAAAACCCTTGCCCGAGATAAAGGCGTTAACTCTTTCAAGATGTTTATGGCCT
  ACAAAGGTCTGTACATGGTGCAAGACGAGCAGCTGTATGCGGCCTTCTCTCAGTGCAAGGAGATAGGAGC
  GATTGCTCAGGTGCACGCCGAGAATGGAGATTTGATTGCAGAGGGAGCAAAGAAGATGCTGGCACTGGGG
  ATAACAGGCCCTGAGGGGCACGAGCTGTGCCGTCCAGAAGCAGTGGAGGCAGAGGCCACCCTGAGAGCCA
  TCACCATAGCCAGCGCTGTGAACTGCCCTTTATACGTCGTGCATGTGATGAGCAAATCTGCAGCGAAGGT
  GGTAGCCGATGCGAGGAGAGCAGGAAATGTGGTCTATGGAGAACCAATTGCAGCGGGTCTCGGCACTGAT
  GGCAGACAGTACTGGAGTGAAGAATGGAGCCATGCAGCCCACCATGTCATGGGTCCCCCACTGAGACCCG
  ATCCTTTAACACCTGGCTTTCTTATGGATCTGTTGGCTAATGGCGATTTGACCACAACAGGGAGTGACAA
  CTGCACTTTTAACACCTGCCAAAAAGCTCTAGGGAAGGATGACTTCACTAAGATTCCCAATGGGGTGAAT
  GGTGTCGAGGACAGGATGTCGGTGATATGGGAAAAGGGCGTGCACAGTGGTAAAATGGATGAAAACAGGT
  TTGTGGCAGTTACCAGCACAAACGCAGCCAAAATCTTTAATCTTTATCCGAAAAAAGGAAGAATAGCTGT
  AGGCTCAGATGCTGACATTGTGATCTGGGACCCAGAAGCAACAAGGAGGATCTCAGCCAAAACTCATCAT
  CAGGCCGTTAACTTCAACATCTTCGAGGGCATGGTTTGTCACGGGGTGCCCCTGGTGACTATTTCAAGAG
  GCAGAGTGGTGTATGAAGCAGGTGTTTTCAACGTCACAGCAGGACATGGGAAGTTTATTCCCCGCCAACC
  CTTTGCCGAATACATTTACAAACGAATCAAGCAGCGAGACCAGACCTGCACACCTGTGCCCGTGAAGCGC
  GCCCCCTACAAGGGAGAAGTCACCACACTGAAAGCCAGAGAGACAAAAGAAGATGACACTGCTGGAACCA
  GGATGCAGGGCCACTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208311 representing NM_022722
  Red=Cloning site Green=Tags(s)
  
  MAPQGRLLIRGGLIVNDDFSQVADVLVEDGVVRALGRDLLPPEDASRGLRILDAAGKLVLPGGIDTHTHM
  QFPFMGSQSVDDFYQGTKAALAGGTTMIIDFAIPQKGSSLIEAFETWRNWADPKVCCDYSLHVAVTWWSD
  KVKEEMKTLARDKGVNSFKMFMAYKGLYMVQDEQLYAAFSQCKEIGAIAQVHAENGDLIAEGAKKMLALG
  ITGPEGHELCRPEAVEAEATLRAITIASAVNCPLYVVHVMSKSAAKVVADARRAGNVVYGEPIAAGLGTD
  GRQYWSEEWSHAAHHVMGPPLRPDPLTPGFLMDLLANGDLTTTGSDNCTFNTCQKALGKDDFTKIPNGVN
  GVEDRMSVIWEKGVHSGKMDENRFVAVTSTNAAKIFNLYPKKGRIAVGSDADIVIWDPEATRRISAKTHH
  QAVNFNIFEGMVCHGVPLVTISRGRVVYEAGVFNVTAGHGKFIPRQPFAEYIYKRIKQRDQTCTPVPVKR
  APYKGEVTTLKARETKEDDTAGTRMQGHS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022722
• ORF Size: 2468 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022722.1
• RefSeq Size: 2468 bp
• RefSeq ORF: 1560 bp
• Locus ID: 64705
• UniProt ID: Q9EQF5
• Cytogenetics: 15 B3.1
• Gene Summary: Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyze the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate (By similarity).[UniProtKB/Swiss-Prot Function]