Hsf2 (NM_008297) Mouse Tagged ORF Clone
CAT#: MG208286
- TrueORF®
Hsf2 (tGFP-tagged) - Mouse heat shock factor 2 (Hsf2)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_008297" in other vectors (3)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Hsf2 |
Synonyms | AI661205 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG208286 representing NM_008297
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCAGAGTTCCAACGTGCCGGCTTTCCTCAGCAAGCTGTGGACGCTTGTGGAGGAAACCCACACCA ACGAGTTCATCACCTGGAGTCAGAATGGACAAAGTTTTCTGGTCTTGGATGAGCAAAGATTTGCAAAGGA AATTCTTCCTAAGTACTTCAAACACAATAACATGGCGAGCTTTGTGAGACAACTAAATATGTATGGCTTC CGAAAAGTAGTGCATATCGAATCTGGAATTATCAAACAGGAAAGAGATGGCCCTGTTGAATTTCAGCATC CTTATTTCAAGCAAGGCCAGGATGACCTGTTGGAGAACATTAAAAGGAAGGTTTCATCTTCAAAACCAGA GGAAAATAAAATTCGTCAGGAAGATTTAACAAAAATTATTAGTAGTGCTCAGAAGGTTCAAATAAAACAA GAAACTATTGAGTCCAGGCTTTCAGAATTAAAAAGTGAGAATGAATCCCTTTGGAAGGAGGTGTCAGAAC TAAGAGCAAAGCATGCCCAGCAGCAACAAGTTATTCGGAAGATTGTCCAGTTTATTGTTACATTGGTTCA GAATAATCAACTTGTGAGTTTAAAACGTAAAAGGCCTCTACTTCTAAACACAAATGGAGCCCCAAAGAAG AATCTATATCAGCACATAGTCAAAGAACCAACTGATAATCACCATCATAAAGTTCCACACAGCAGGACTG AAGGTTTAAAGTCAAGAGAACGGATTTCAGATGACATAATTATTTATGATGTTACTGACGATAATGTGGA TGAAGAAAATATTCCAGTTATTCCAGAAACAAATGAGGATGTTGTAGTGGATTCCTCCAACCAGTATCCT GACATTGTCATTGTTGAAGATGACAACGAGGATGAGTATGCTCCTGTCATTCAGAGTGGAGAGCAGAGTG AACCAGCCAGAGAACCCTTACGTGTGGGGAGTGCTGGCAGCAGCAGCCCTCTCATGTCCAGTGCTGTCCA GCTAAACGGCTCCTCCAATCTGACCTCAGACGACCCTGTGACCATGATGGACTCCATTCTGAATGACAAC ATTAACCTGTTAGGAAAGGTTGAGCTGTTGGATTACCTTGACAGTATTGATTGCAGTTTAGAGGACTTCC AAGCTATGCTCTCAGGAAGACAGTTTAGCATAGACCCAGATCTTCTGGTTGATTCTGAGAATAAGGGACT AGAAGCTACCAAGAGCAGTGTTGTTCAACACGTGTCAGAAGAGGGAAGAAAATCTAAATCCAAGCCAGAC AAACAACTTATCCAGTATACTGCCTTTCCACTTCTTGCATTCCTGGATGGGAACTCTGCATCTGCTATTG AACAGGGGAGTACAACTGCATCGTCAGAAGTTGTGCCTTCTGTAGATAAGCCCATAGAGGTCGATGAGCT CCTGGATAGCAGCCTGGATCCAGAACCGACCCAGAGTAAGCTTGTCCGCCTGGAACCATTGACTGAAGCG GAAGCTAGTGAAGCCACACTCTTCTATTTATGTGAACTTGCTCCTGCACCTCTGGATAGTGATATGCCGC TTTTAGATAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208286 representing NM_008297
Red=Cloning site Green=Tags(s) MKQSSNVPAFLSKLWTLVEETHTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMASFVRQLNMYGF RKVVHIESGIIKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQ ETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAPKK NLYQHIVKEPTDNHHHKVPHSRTEGLKSRERISDDIIIYDVTDDNVDEENIPVIPETNEDVVVDSSNQYP DIVIVEDDNEDEYAPVIQSGEQSEPAREPLRVGSAGSSSPLMSSAVQLNGSSNLTSDDPVTMMDSILNDN INLLGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDSENKGLEATKSSVVQHVSEEGRKSKSKPD KQLIQYTAFPLLAFLDGNSASAIEQGSTTASSEVVPSVDKPIEVDELLDSSLDPEPTQSKLVRLEPLTEA EASEATLFYLCELAPAPLDSDMPLLDS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008297 |
ORF Size | 1551 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008297.2, NP_032323.2 |
RefSeq Size | 2514 bp |
RefSeq ORF | 1554 bp |
Locus ID | 15500 |
UniProt ID | P38533 |
Cytogenetics | 10 B4 |
Gene Summary | DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF2 is expressed in a form that binds DNA constitutively but loses DNA binding by incubation at greater than 41 degrees C.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR208286 | Hsf2 (Myc-DDK-tagged) - Mouse heat shock factor 2 (Hsf2) |
USD 482.00 |
|
MR208286L3 | Lenti ORF clone of Hsf2 (Myc-DDK-tagged) - Mouse heat shock factor 2 (Hsf2) |
USD 782.00 |
|
MR208286L4 | Lenti ORF clone of Hsf2 (mGFP-tagged) - Mouse heat shock factor 2 (Hsf2) |
USD 782.00 |
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