Hars (NM_008214) Mouse Tagged ORF Clone

CAT#: MG208184

Hars (tGFP-tagged) - Mouse histidyl-tRNA synthetase (Hars)

Product Images

Circular map for MG208184

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hars
• Synonyms: MMHRS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208184 representing NM_008214
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGATCGTGCTGCTCTGGAGGAGCTGGTACGACTCCAGGGAGCGCATGTGAGGGGCCTTAAGGAGC
  AGAAAGCGTCCGCTGAGCAGATCGAGGAGGAGGTGACGAAACTCCTAAAACTGAAGGCACAGCTGGGCCA
  GGATGAAGGCAAACAGAAGTTTGTGCTCAAAACCCCCAAGGGCACGAGAGACTATAGTCCCCGGCAGATG
  GCAGTCCGGGAAAAGGTGTTTGATGTCATCATCCGCTGTTTCAAACGCCATGGAGCAGAAGTGATTGATA
  CACCTGTGTTTGAACTAAAGGAAACACTGACTGGAAAGTACGGAGAAGACTCAAAGCTTATCTATGACTT
  GAAGGACCAGGGTGGGGAGCTGCTGTCCCTCCGTTATGACCTTACTGTCCCTTTTGCTCGCTATCTGGCA
  ATGAATAAACTGACCAACATTAAACGCTACCATATAGCAAAGGTGTATCGCCGGGACAACCCAGCTATGA
  CCCGAGGCCGTTACCGTGAATTCTATCAATGCGATTTTGACATTGCCGGACAGTTCGACCCCATGATTCC
  TGATGCAGAGTGCCTAAAGATTATGTGCGAGATCCTGAGTTCACTTCAGATCGGCAACTTCCTGGTCAAG
  GTAAACGACAGGCGCATCCTAGATGGGATGTTTGCTGTCTGTGGTGTTCCTGATAGCAAGTTCCGTACCA
  TCTGCTCCTCGGTGGACAAGCTAGACAAGGTTTCCTGGGAGGAAGTGAAGAATGAGATGGTGGGAGAGAA
  GGGCCTTGCACCGGAAGTGGCTGATCGCATCGGGGACTATGTCCAGCAGCATGGTGGGGTTTCTCTGGTG
  GAGCAGTTACTGCAGGATCCTAAACTCTCCCAAAACAAACAGGCTTTAGAGGGCTTGGGAGACCTAAAGC
  TGCTCTTTGAGTATCTAATCCTGTTTGGCATTGATGACAAGATCTCCTTTGACCTGAGCCTTGCTCGGGG
  GCTAGACTACTATACTGGGGTCATCTATGAGGCAGTGCTGCTACAGATGCCAACCCAAGCTGGGGAAGAG
  CCCCTGGGTGTGGGCAGCATAGCTGCTGGAGGGCGCTACGATGGCCTGGTTGGCATGTTTGACCCCAAGG
  GGCGCAAGGTGCCATGCGTTGGGCTCAGTATTGGAGTGGAGCGGATCTTTTCCATTGTAGAGCAAAGATT
  AGAGGCTTCAGAGGAAAAGGTACGGACCACAGAGACACAGGTGCTTGTGGCATCTGCACAGAAGAAGCTG
  CTGGAGGAGAGACTGAAGCTTGTCTCAGAGTTATGGGATGCTGGGATCAAGGCTGAGCTGCTGTACAAGA
  AGAACCCGAAGTTACTAAACCAGTTGCAGTACTGTGAGGAGGCAGGCATCCCACTGGTGGCCATCATTGG
  TGAGCAGGAACTCAAGGATGGCGTCATCAAGCTCCGCTCGGTGGCCAGCAGAGAGGAGGTGGATGTCCGA
  AGAGAAGACCTTGTGGAGGAAATCAGAAGAAGAACAAATCAGCCCCTCTCCATCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208184 representing NM_008214
  Red=Cloning site Green=Tags(s)
  
  MADRAALEELVRLQGAHVRGLKEQKASAEQIEEEVTKLLKLKAQLGQDEGKQKFVLKTPKGTRDYSPRQM
  AVREKVFDVIIRCFKRHGAEVIDTPVFELKETLTGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLA
  MNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGQFDPMIPDAECLKIMCEILSSLQIGNFLVK
  VNDRRILDGMFAVCGVPDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLV
  EQLLQDPKLSQNKQALEGLGDLKLLFEYLILFGIDDKISFDLSLARGLDYYTGVIYEAVLLQMPTQAGEE
  PLGVGSIAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEASEEKVRTTETQVLVASAQKKL
  LEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVASREEVDVR
  REDLVEEIRRRTNQPLSIC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008214
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008214.2, NP_032240.2
• RefSeq Size: 1903 bp
• RefSeq ORF: 1530 bp
• Locus ID: 15115
• UniProt ID: Q61035
• Cytogenetics: 18 B2
• Gene Summary: Cytoplasmic histidine--tRNA ligase. Plays a role in axon guidance.[UniProtKB/Swiss-Prot Function]