Trim31 (NM_146077) Mouse Tagged ORF Clone
CAT#: MG208148
- TrueORF®
Trim31 (tGFP-tagged) - Mouse tripartite motif-containing 31 (Trim31)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_146077" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Trim31 |
Synonyms | BC026666; HCG1; HCGI; RNF |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG208148 representing NM_146077
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGCCAACCTCTGGCCTGCCAACTGCAGGAGGAGGTGACCTGCCCCATCTGCATGGAAATTCTAC AAGATCCTGTCACCATTGACTGTGGGCACAACTTCTGCCTGCAGTGTATCAGTCAGGTTGGAAAAACGTC AGAAAAGATCCAATGTCCTCTCTGCAAACTCTCTGTGAATAAGAATACGTTCAGGCCCAATAAGCTGCTG GCTAGTCTTGCAGAGAAGATCCAAAGTATGGATCCTGCTGACATCCAAGCAGAAAAGGAAGACTCAAGAT GTCAGAGGCACAAGGAAAAGCTACATTACTTCTGCGAGCAGGATGGGGCATTCCTCTGTGTGGTGTGTCG TGACTCCAAGGACCACAAGTCTCACAATGTCACCTTGATAGATGAGGCTGCCCAGAACTACAAGGTGCAG ATTGAGTCACAGGCCCAAGATTTGGGGCAAAAGGACAAGAAAATAATTGAAGAGAAAAAACAAGGTGAAG GGGCAATCTGGGCGTTCAGGGCCCAGGTGGATCTAGAGAAACTAAAGATCCATGAGGAATTCAAGCTCCT GCGCCAGAGACTAGATGAGGAAGAGAGTTTCCTCCTGTCCAGGCTGGACTGGCTGGAGCAGCAGGGAGCC AAGCAGTTGAGACAATATGTCACTGTGACAGAGAAGCAGCTGAACTCTCTAAGGAAGCTCACTAAATCCT TGAAAATAAGGCTGCAATCATCATCCATGGAGCTACTAAAGGACATTAAAGACGCCTTGAGCAGGGGTAA GGAATTTCAGTTTCTCAACCCAAACCCAGTTCCTGAGGACCTGGAGAAAAAATGCAGTGAAGCAAAAGCA AGGCATGAGTCCATCATAAAAACCCTGACAGAACTCAAAGACGATATGAATGCCGAAGGGAAGAGAGATA AAAGCGCATTCATGAACAGCCTGAATAAAGAAGAAAAGGAGAGCTGGAGCCTGTTACAGAAGAATAATTC AGTGTTGCCCACCTCAGTCCCTGTGACCCTGGACAAGTCCTCAGCTGACCCAGACCTCACCTTTTCTCAG GATCTAAAGAAAGTGACCTTGTATATTGTAGCTGGAAAAGCTTCCAATAGGCAGGCAAAACCTCGACCAT TCTACCCTTTCCACTGTGTGAGGGGTTCCCCAGGCCTCTCCTCAGGCCGCCAGGTGTGGGAGGCAGAAAT CCGGGGGCCCTCAGGTGGGGCATGCATTGTGGGCGTGGTTACAGAGTTGGCGCGAGGGGCCCAGAGTCAA ACCGTGAGCGCGCAGAGCTACATATGGGCACTGCGGATCTCGCCCTCTGGATGCCAGCCATTCACCAATT GTAAAGCCCAGGAGTACCTCCAGGTCTGTCTTAAGAAAGTGGGTGTCTATGTGAATCATGACTGTGGAGA GGTCGTCTTCTATGATGCCATCACTAGCAAACACATCTATACTTTCCAAACTTCCTTTGACGGGAAGGTC TTCCCCCTTTTCGGGCTCCAAGTTGCCTGCAGCCATATCACCCTGAGCCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208148 representing NM_146077
Red=Cloning site Green=Tags(s) MAGQPLACQLQEEVTCPICMEILQDPVTIDCGHNFCLQCISQVGKTSEKIQCPLCKLSVNKNTFRPNKLL ASLAEKIQSMDPADIQAEKEDSRCQRHKEKLHYFCEQDGAFLCVVCRDSKDHKSHNVTLIDEAAQNYKVQ IESQAQDLGQKDKKIIEEKKQGEGAIWAFRAQVDLEKLKIHEEFKLLRQRLDEEESFLLSRLDWLEQQGA KQLRQYVTVTEKQLNSLRKLTKSLKIRLQSSSMELLKDIKDALSRGKEFQFLNPNPVPEDLEKKCSEAKA RHESIIKTLTELKDDMNAEGKRDKSAFMNSLNKEEKESWSLLQKNNSVLPTSVPVTLDKSSADPDLTFSQ DLKKVTLYIVAGKASNRQAKPRPFYPFHCVRGSPGLSSGRQVWEAEIRGPSGGACIVGVVTELARGAQSQ TVSAQSYIWALRISPSGCQPFTNCKAQEYLQVCLKKVGVYVNHDCGEVVFYDAITSKHIYTFQTSFDGKV FPLFGLQVACSHITLSP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_146077 |
ORF Size | 1521 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_146077.2, NP_666189.1 |
RefSeq Size | 2248 bp |
RefSeq ORF | 1524 bp |
Locus ID | 224762 |
UniProt ID | Q8R0K2 |
Cytogenetics | 17 B1 |
Gene Summary | May have E3 ubiquitin-protein ligase activity (By similarity). Regulator of Src-induced anchorage independent cell growth.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217203 | Trim31 (untagged) - Mouse tripartite motif-containing 31 (Trim31), (10ug) |
USD 520.00 |
|
MR208148 | Trim31 (Myc-DDK-tagged) - Mouse tripartite motif-containing 31 (Trim31) |
USD 473.00 |
|
MR208148L3 | Lenti ORF clone of Trim31 (Myc-DDK-tagged) - Mouse tripartite motif-containing 31 (Trim31) |
USD 773.00 |
|
MR208148L4 | Lenti ORF clone of Trim31 (mGFP-tagged) - Mouse tripartite motif-containing 31 (Trim31) |
USD 773.00 |
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