Pde7a (BC062909) Mouse Tagged ORF Clone
CAT#: MG207723
- TrueORF®
Pde7a (tGFP-tagged) - Mouse phosphodiesterase 7A (cDNA clone MGC:86015 IMAGE:6815827)
Frequently bought together (4)
Other products for "Pde7a"
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Pde7a |
Synonyms | AU015378; AW047537 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG207723 representing BC062909
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTATGCTACCAGCTGCCGGTGCTGCCCCTGGACAGGCCGGTCCCCCAGCACGTCCTCAGCCGCC GAGGAGCCATCAGCTTCAGCTCCAGCTCCGCGCTCTTCGGCTGCCCGCATCCCCGGCAGCTCTCGCAGAG ACGTGGAGCTATTTCCTATGACAGTTCTGATCAGACTGCGTTATATATTCGTATGCTAGGAGATGTCCGC GTGAGGAGCCGAGCAGGATTTGAAACAGAAAGAAGAGGTTCCCATCCGTACATTGACTTCCGTATTTTTC ACTCTCAATCTGACATTGAAGCATCAGTCTCCGCCAGGAACATCAGAAGGTTACTAAGTTTCCAGCGATA TCTCAGATCCTCACGAGTTTTTCGGGGTGCCACAGTTTGTAGTTCTCTAGACATTTTAGATGAGGATTAC AATGGACAAGCCAAGTGTATGCTGGAAAAAGTTGGAAATTGGAATTTTGATATCTTTCTGTTTGATAGAC TAACAAATGGAAATAGTCTAGTAAGCCTAACCTTTCATTTATTTAGTCTTCATGGATTGATTGAGTACTT CCATTTAGATATGGTGAAACTCCGTCGGTTTTTAGTTATGATTCAAGAAGATTACCACAGTCAAAATCCT TACCACAATGCAGTCCATGCTGCAGACGTTACTCAGGCCATGCACTGTTACTTAAAGGAACCTAAGCTTG CCAGTTCTGTCACTCCTTGGGATATCTTGCTGAGTTTAATTGCAGCCGCCACTCACGATCTGGATCACCC AGGTGTTAATCAGCCGTTTCTTATTAAAACCAACCATTATCTAGCAACTTTATACAAGAATTCCTCAGTC CTGGAGAATCACCACTGGAGATCTGCAGTGGGCTTGTTAAGAGAATCTGGTTTGTTCTCACACTTGCCAT TGGAAAGCAGGCAAGAGATGGAGGCTCAGATAGGTGCTTTGATATTAGCCACGGATATCAGTCGCCAGAA CGAGTACCTGTCATTGTTTAGATCTCACTTGGATAAAGGTGACTTACACCTTGACGATGGCGGACATAGG CATTTGGTTCTACAGATGGCCTTGAAATGTGCTGATATTTGTAACCCATGTCGGAACTGGGAATTAAGCA AGCAGTGGAGTGAAAAAGTAACGGAGGAATTCTTCCACCAAGGAGATATAGAAAAAAAGTACCATTTGGG TGTGAGTCCACTTTGTGATCGTCAGACTGAGTCTATTGCCAACATCCAGATTGGTTTTATGACTTACCTA GTGGAGCCTTTATTTACAGAGTGGGCCAGGTTTTCAGACACACGGCTGTCACAGACGATGCTTGGACATG TGGGGCTGAATAAAGCCAGCTGGAAGGGACTGCAGAGACAACAGCCCAGCAGCGAGGATGCCAATGCTGC ATTTGAGTTGAACTCACAGTTACTAACTCAGGAAAATCGGTTATCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207723 representing BC062909
Red=Cloning site Green=Tags(s) MEVCYQLPVLPLDRPVPQHVLSRRGAISFSSSSALFGCPHPRQLSQRRGAISYDSSDQTALYIRMLGDVR VRSRAGFETERRGSHPYIDFRIFHSQSDIEASVSARNIRRLLSFQRYLRSSRVFRGATVCSSLDILDEDY NGQAKCMLEKVGNWNFDIFLFDRLTNGNSLVSLTFHLFSLHGLIEYFHLDMVKLRRFLVMIQEDYHSQNP YHNAVHAADVTQAMHCYLKEPKLASSVTPWDILLSLIAAATHDLDHPGVNQPFLIKTNHYLATLYKNSSV LENHHWRSAVGLLRESGLFSHLPLESRQEMEAQIGALILATDISRQNEYLSLFRSHLDKGDLHLDDGGHR HLVLQMALKCADICNPCRNWELSKQWSEKVTEEFFHQGDIEKKYHLGVSPLCDRQTESIANIQIGFMTYL VEPLFTEWARFSDTRLSQTMLGHVGLNKASWKGLQRQQPSSEDANAAFELNSQLLTQENRLS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC062909 |
ORF Size | 1448 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC062909, AAH62909 |
RefSeq Size | 3384 bp |
RefSeq ORF | 1448 bp |
Locus ID | 18583 |
Cytogenetics | 3 5.36 cM |
Gene Summary | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction (By similarity).[UniProtKB/Swiss-Prot Function] |
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