Lbp (NM_008489) Mouse Tagged ORF Clone

CAT#: MG207709

Lbp (tGFP-tagged) - Mouse lipopolysaccharide binding protein (Lbp)

Product Images

Circular map for MG207709

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lbp
• Synonyms: Bpifd2; Ly88
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207709 representing NM_008489
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCTGGGACTGGACCTCTGCTCTCTACATTGCTGGGGCTGCTGTTCCTGTCCATACAGGGAACTG
  GCGGTGTCAACCCCGGTGTGGTGGCCAGGATCACCGACAAGGGCCTGGCCTACGCGGCCAAGGAGGGGCT
  GGTGGCTCTGCAGAGAGAGCTGTACAAGATCACACTACCGGACTTCAGCGGGGACTTCAAGATCAAGGCC
  GTGGGTCGTGGGCAGTACGAGTTTCATAGCCTGGAGATTCAGAACTGTGAGCTGCGTGGCTCCTCTCTGA
  AGCTGCTCCCAGGCCAGGGCCTGAGTCTCGCCATCTCTGACTCTTCCATCGGTGTCCGAGGCAAATGGAA
  GGTGCGCAAATCCTTCCTGAAACTTCATGGCTCCTTTGACCTGGATGTCAAAGGTGTCACCATTTCAGTG
  GACCTCCTCCTGGGCATGGATCCCTCAGGGCGGCCCACAGTCTCTGCCTCTGGGTGCAGCAGCCGCATTT
  GTGATTTGGATGTGCACATATCAGGAAATGTGGGGTGGCTGCTGAATCTCTTCCACAACCAGATCGAGTC
  GAAGCTCCAAAAAGTACTGGAGAATAAGGTTTGTGAGATGATCCAGAAGTCTGTGACCTCTGACCTGCAG
  CCTTATCTCCAGACTCTGCCAGTCACAGCAGAGATCGACAATGTCCTGGGCATTGACTACAGTTTGGTGG
  CGGCTCCCCAAGCGAAGGCCCAGGTGCTGGATGTCATGTTTAAGGGTGAAATTTTTAATCGGAATCACCG
  CTCTCCAGTTGCTACCCCCACTCCGACCATGAGCCTACCTGAGGACAGTAAGCAAATGGTCTACTTTTCC
  ATCTCAGATCATGCCTTCAACATAGCAAGCCGGGTTTATCACCAGGCTGGGTACCTGAACTTTTCCATCA
  CAGATGACATGTTACCACCTGACTCCAGCATCCGGTTGAACACCAAGGCCTTCCGTCCCTTCACTCCCCA
  GATATACAAAAAGTATCCCGACATGAAACTGGAGCTCCTTGGAACAGTGGTCTCTGCCCCGATTCTGAAT
  GTCAGTCCTGGGAATCTGTCCTTGGCCCCACAGATGGAGATTGAAGGCTTTGTGATCCTGCCCACCTCCG
  CCCGGGAACCTGTCTTCCGGCTTGGCGTGGTCACTAATGTATTTGCCTCATTAACTTTCAACAACAGCAA
  GGTTACCGGGATGCTGCACCCAGACAAGGCACAAGTGAGACTGATCGAATCCAAAGTCGGCATGTTCAAT
  GTGAACCTGTTCCAGGCATTCCTTAACTACTACCTTCTCAACAGCCTCTACCCTGATGTCAATGCTGAGC
  TGGCCCAGGGCTTCCCCCTCCCTCTGCCAAGGCATATTCAGCTCCACGACCTCGACTTCCAGATCCGCAA
  GGACTTCTTATACTTGGGTGCCAATGTCCAGTACATGAGAGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207709 representing NM_008489
  Red=Cloning site Green=Tags(s)
  
  MKAGTGPLLSTLLGLLFLSIQGTGGVNPGVVARITDKGLAYAAKEGLVALQRELYKITLPDFSGDFKIKA
  VGRGQYEFHSLEIQNCELRGSSLKLLPGQGLSLAISDSSIGVRGKWKVRKSFLKLHGSFDLDVKGVTISV
  DLLLGMDPSGRPTVSASGCSSRICDLDVHISGNVGWLLNLFHNQIESKLQKVLENKVCEMIQKSVTSDLQ
  PYLQTLPVTAEIDNVLGIDYSLVAAPQAKAQVLDVMFKGEIFNRNHRSPVATPTPTMSLPEDSKQMVYFS
  ISDHAFNIASRVYHQAGYLNFSITDDMLPPDSSIRLNTKAFRPFTPQIYKKYPDMKLELLGTVVSAPILN
  VSPGNLSLAPQMEIEGFVILPTSAREPVFRLGVVTNVFASLTFNNSKVTGMLHPDKAQVRLIESKVGMFN
  VNLFQAFLNYYLLNSLYPDVNAELAQGFPLPLPRHIQLHDLDFQIRKDFLYLGANVQYMRV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008489
• ORF Size: 1443 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008489.2, NP_032515.2
• RefSeq Size: 2528 bp
• RefSeq ORF: 1446 bp
• Locus ID: 16803
• UniProt ID: Q61805
• Cytogenetics: 2 78.72 cM
• Gene Summary: Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria (PubMed:9144073). Acts as an affinity enhancer for CD14, facilitating its association with LPS (By similarity). Promotes the release of cytokines in response to bacterial lipopolysaccharide (PubMed:24380872).[UniProtKB/Swiss-Prot Function]