Osgin1 (BC022135) Mouse Tagged ORF Clone

CAT#: MG207664

Osgin1 (tGFP-tagged) - Mouse oxidative stress induced growth inhibitor 1 (cDNA clone MGC:37844 IMAGE:5099665)

Product Images

Circular map for MG207664

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Osgin1
• Synonyms: 1700012B18Rik; Okl38
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207664 representing BC022135
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTCCTGGAGGCACGACTCCCTTGGGGCCAGCAGCTCAGAGCCCCTCCCAGTAGTCATCATCGGCA
  ATGGCCCCTCCGGTATCTGCCTGTCCTACCTGCTCTCTGGACACATCCCCTACGTGAAACCAGGAGCCGT
  CCACCCACACCCCCTGCTGCAGAGAAAGCTGGCCGAGGCTCCAGGGGTCTCCATCCTGGACCAGGACCTA
  GAGTACCTTTCCGAAGGCCTCGAAGGCCGAAGCCAGAGTCCTGTGGCCCTGCTCTTTGATGCCCTCCTGC
  GTCCTGACACAGACTTTGGAGGCAGCATAGACTCTGTGCTCTCCTGGAAGCGGCAGAAGGACCGCGCTGT
  TCCCCACCTTGTCCTGGGACGGAACCTCCCCGGGGGCGCCTGGCATTCCATTGAAGGCTCCATGGTGACC
  TTGAGCCAAGGCCAGTGGATGAGTCTCCCAGACCTGCAGGTCAAAGACTGGATGCGGAAGAAATGCAGAG
  GTCTCCGCAACAGCAGAGCCACAGCCGGTGACATCGCCCACTACTACCGGGACTACGTGATCAAGAAAGG
  CCTAAGTCACAACTTTGTGTCTGGTGCCGTGGTCACTGCCGTGGAGTGGGCTAAGTCCGAGCATGGCAGT
  CCCGAGGTCCAGGCATCCAGTCCCCTCTTCCAAGTGAATGGCTACTTGACTACCAAGGACCACGGCCACC
  AGCCCTTCTCACTGCGGGCCCGTAATGTGGTCCTGGCCACAGGCACCTTTGACAGCCCAGCCATGCTAGG
  CATCCCAGGAGAGACCCTGCCCTTTGTCCACCACGACCTGTCAGCCCTGGAGGCAGCCCTTCGGGCAGGC
  ACTGTGAACCCAACCTCAGACCCGGTGCTGATTGTGGGAGCAGGGCTATCTGCAGCCGATGCTGTCCTCT
  TTGCCCGTCACTACAACATCCAGGTGATCCATGCTTTCCGCCGGTCCGTGCATGACCCCGGCCTGGTATT
  CAACCAGCTGCCCAAGATGCTATACCCTGAGTACCACAAAGTGCAGCAGATGATGCGCGATCAGTCCATC
  TTGTCTCCTAGCCCCTATGAGGGCTACCGCAGCCTCCCTGAGCACCAGCCACTGCTCTTCAAAGAGGACC
  ACCAAGCAGTGTTCCAGGACCCACAGGGGGGCCAGCAGCTCTTTGGGGTCTCCATGGTGCTGGTCCTCAT
  TGGCTCCCACCCCGACCTCTCCTACCTCCCCAGGGCAGGTGCTGACTTGGTCATAGACCCAGATCAGCCA
  CTGAGTCCCAAGAGGAACCCCATTGACGTGGACCCCTTCACCCATGAGAGCACTCACCAGGAGGGCCTGT
  ATGCCCTGGGGCCGTTGGCTGGGGACAACTTTGTGAGATTTGTCCAGGGCGGGGCCCTGGCTGCTGCCAG
  CTCCCTGCTGAAGAAGGAGACCAGGAAGCCACCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207664 representing BC022135
  Red=Cloning site Green=Tags(s)
  
  MTSWRHDSLGASSSEPLPVVIIGNGPSGICLSYLLSGHIPYVKPGAVHPHPLLQRKLAEAPGVSILDQDL
  EYLSEGLEGRSQSPVALLFDALLRPDTDFGGSIDSVLSWKRQKDRAVPHLVLGRNLPGGAWHSIEGSMVT
  LSQGQWMSLPDLQVKDWMRKKCRGLRNSRATAGDIAHYYRDYVIKKGLSHNFVSGAVVTAVEWAKSEHGS
  PEVQASSPLFQVNGYLTTKDHGHQPFSLRARNVVLATGTFDSPAMLGIPGETLPFVHHDLSALEAALRAG
  TVNPTSDPVLIVGAGLSAADAVLFARHYNIQVIHAFRRSVHDPGLVFNQLPKMLYPEYHKVQQMMRDQSI
  LSPSPYEGYRSLPEHQPLLFKEDHQAVFQDPQGGQQLFGVSMVLVLIGSHPDLSYLPRAGADLVIDPDQP
  LSPKRNPIDVDPFTHESTHQEGLYALGPLAGDNFVRFVQGGALAAASSLLKKETRKPP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC022135
• ORF Size: 1436 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC022135, AAH22135
• RefSeq Size: 1982 bp
• RefSeq ORF: 1436 bp
• Locus ID: 71839
• Cytogenetics: 8 E1
• Gene Summary: Regulates the differentiation and proliferation through the regulation of cell death.[UniProtKB/Swiss-Prot Function]