Frmd8 (NM_026169) Mouse Tagged ORF Clone

CAT#: MG207454

Frmd8 (tGFP-tagged) - Mouse RIKEN cDNA 1200004M23 gene (1200004M23Rik)

Product Images

Circular map for MG207454

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Frmd8
• Synonyms: 1200004M23Rik; 2310035N23Rik; 4931429L16Rik; AU018809
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207454 representing NM_026169
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGGGCAGAAGGCAACGCGGGGCAGCCCGGCCCTGCGGAGCGGTCCCACCGAAGTAGCGTCTCTT
  CAGTGGGTGCCCGAGCAGCCGATGTCCTGGTGTACCTGGCGGATGATACAGTAGTGCCCCTGGCCGTGGA
  GAATTTGTCCTCAATCAGTGCCCATGAGCTGCACCGCGCAGTCCGTGAAGTCCTGCAGCTCCCAGACGTG
  GCTTTGGAGGCCTTTGCACTCTGGCTCGTCTCCCCTCTCTTGGAGGTGCAATTGAAACCCAAGCACCAGC
  CCTATAAACTGGGCCGCCAGTGGCCGGAGCTGCTGCTGCGCTTCACCGACGCCTCCGATGATGATGTCGC
  CATGGATGAGCCTTCCCTTCAGTTCCGAAGGAATGTTTTTTTCCCAAGGCGCCGAGAGCTCCAGATCCAT
  GATGAGGAGGTCTTGCGGCTGCTCTATGAGGAAGCCAAGGGCAATGTGCTGACCGCTCGGTACCCTTGTG
  ACTTGGAGGACTGCGAGGTGCTGGGTGGCCTTGTGTGCCGTGTGCAGCTTGGGCCCTACCAGCCTGGCCA
  GCCTGCCGCCTGCACTCTGAGAGAGAAGCTGGACTCCTTCCTCCCTGCCCACCTCTGCAAGCGAGGGCAT
  GGCCTCTTTGCTGCCTTCCGGGGCCGTGGGGCCAAGACTGGGCCAGGTGAGCAAGGCCTGCTGAATGCCT
  ACCGCCAGGTAAAGGAAGTGACAGGCAACAACAGTGAACGTGAGGCCACCTTGGGCTCCCACTACCGCGC
  ATACCTCCTCAAGTGCCACGAGCTGCCCTTCTACGGGTGTGCCTTCTTCCATGGCGAGGTTGACAAGCCA
  GCCCAGGGCTTCTTGCATCGAGGTGGGCGCAAACCAGTGACTGTGGCCATCAGTCTGGAGGGTGTGCACG
  TCATCGACAACAGGGAGAAGCATGTGCTGCTGGGCCTGCGCTTTCAGGAGCTGTCGTGGGATCACACTTC
  CCCTGAGGAGGAAGAGCCTGTCCTGTGGCTGGAGTTCGATGGTGACAGTGAGGGCACGCCTGTCAACAAG
  CTGCTAAGGATCTACTCCAAGCAGGCTGAGCTGATGAGCGGCCTCATTGAGTACTGCATTGAGCTGAGCC
  AGGCTGCCGAGCCTACTCTTTCCCAGGAGAGTGCATCCGGCCCTCACGAAGCTCCCAGCCCCTCTCCACC
  TCCCACCCAGCGCCCCAAGCTGCGAAGGCAGGGTAGCGTGGTGTGTAGCCGGATTCAGCATCTCTCTACC
  ATTGACTATGTGGAAGACGGCAAGGGGATCAAGCGGGTGAAACCAAAGCGAACCACCTCCTTCTTCAGCC
  GACAGCTCTCCTCCAGCCAGGGAAGCTACACCGTGGTGCAGCCCACCGATGACAGCCTGGAGCAGAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207454 representing NM_026169
  Red=Cloning site Green=Tags(s)
  
  MEGAEGNAGQPGPAERSHRSSVSSVGARAADVLVYLADDTVVPLAVENLSSISAHELHRAVREVLQLPDV
  ALEAFALWLVSPLLEVQLKPKHQPYKLGRQWPELLLRFTDASDDDVAMDEPSLQFRRNVFFPRRRELQIH
  DEEVLRLLYEEAKGNVLTARYPCDLEDCEVLGGLVCRVQLGPYQPGQPAACTLREKLDSFLPAHLCKRGH
  GLFAAFRGRGAKTGPGEQGLLNAYRQVKEVTGNNSEREATLGSHYRAYLLKCHELPFYGCAFFHGEVDKP
  AQGFLHRGGRKPVTVAISLEGVHVIDNREKHVLLGLRFQELSWDHTSPEEEEPVLWLEFDGDSEGTPVNK
  LLRIYSKQAELMSGLIEYCIELSQAAEPTLSQESASGPHEAPSPSPPPTQRPKLRRQGSVVCSRIQHLST
  IDYVEDGKGIKRVKPKRTTSFFSRQLSSSQGSYTVVQPTDDSLEQS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026169
• ORF Size: 3163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026169.3
• RefSeq Size: 3169 bp
• RefSeq ORF: 1401 bp
• Locus ID: 67457
• UniProt ID: Q3UFK8
• Cytogenetics: 19 A
• Gene Summary: Promotes the cell surface stability of iRhom1/RHBDF1 and iRhom2/RHBDF2 and prevents their degradation via the endolysosomal pathway (PubMed:29897333). By acting on iRhoms, involved in ADAM17-mediated shedding of TNF, amphiregulin/AREG, HBEGF and TGFA from the cell surface (By similarity). Negatively regulates Wnt signaling, possibly by antagonizing the recruitment of AXIN1 to LRP6 (By similarity).[UniProtKB/Swiss-Prot Function]