Tmcc3 (BC057202) Mouse Tagged ORF Clone

CAT#: MG207116

Tmcc3 (tGFP-tagged) - Mouse transmembrane and coiled coil domains 3 (cDNA clone MGC:67743 IMAGE:5327878)

Product Images

Circular map for MG207116

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tmcc3
• Synonyms: MGC30586
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207116 representing BC057202
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATACCCTCAGCCTACCCCTGAACATACGCCGAGGGGGATCAGACACCAACCTCAACTTCGATGTCC
  CCGATGGCATCCTGGATTTCCACAAGGTCAAACTCAATGCGGACAGCCTGAGACAAAAAATCTTAAAGGT
  GACGGAGCAGATAAAGATCGAGCAGACGTCCCGAGATGGGAACGTTGCAGAGTATCTGAAACTGGTCAGC
  AGTGCAGACAAGCAGCAGGCTGGACGCATCAAGCAGGTCTTTGAGAAGAAAAACCAGAAGTCAGCCCACT
  CCATCGCCCAACTTCAGAAGAAGTTGGAGCAGTATCACAGAAAGCTCAGGGAGATCGAACAGAATGGCGT
  GACCAGGAGCTCAAAGGACATCTCTAAAGACAGCCTGAAGGAAATACACCACTCTCTGAAGGATGCCCAC
  GTGAAATCTCGAACTGCTCCCCACTGCCTGGAGAGCAGTAAATCTAGCATGCCAGGGGTATCCCTCACGC
  CCCCCGTGTTTGTCTTCAATAAGTCCAGAGAGTTTGCCAACTTGATCCGGAATAAGTTTGGCAGTGCAGA
  TAACATTGCTCACTTGAAGAATTCCCTGGAAGAGTTCAGGCCCGAAGCCAGCCCCAGGGCTTACGGCGGA
  AGCGCTACCATCGTGAACAAACCTAAGTACGGCAGCGATGACGAATGTTCTAGTGGCACATCCGGCTCAG
  CGGACAGCAATGGGAACCAGTCCTTTGGGGCTGGTGGGACCAGCACCCTGGACAGCCAGGGGAAGATTGC
  CAAGATCATGGAGGAACTGAGGGAGATCAAGGTTACCCAAACCCAGCTGGCGGAAGACATCGAGGCACTG
  AAGGTGCAGTTTAAGAGAGAATATGGCTTTATCTCTCAGACTCTGCAAGAGGAGAGATACAGGTATGAGC
  GACTTGAAGACCAGCTCCATGACCTGACAGAGCTGCACCAGCATGAGACAGCCAATCTGAAGCAGGAATT
  GGCCAGCGCTGAGGAGAAGGTGGCCTACCAGGCCTATGAGCGCTCAAGGGACATCCAGGAAGCCTTGGAA
  TCTTGCCAGACCCGCATCTCTAAGCTCGAGCTGCATCAGCAGGAACAGCAGACCCTGCAGACGGATGCCG
  TGAACGCCAAGGTCCTGCTGGGGAAGTGCATCAACGTGGTTCTGGCCTTCATGACGGTCATCCTGGTGTG
  TGTGTCCACCCTGGCCAAGTTTGTCTCGCCCATGATGAAGAGCCGCTCCCACATCCTTGGCACCTTCTTT
  GCTGTGACTCTTCTCGCCATATTCTGTAAAAACTGGGACCATATCTTGTGTGCCATAGAAAGGATAATCA
  TCCCGAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207116 representing BC057202
  Red=Cloning site Green=Tags(s)
  
  MNTLSLPLNIRRGGSDTNLNFDVPDGILDFHKVKLNADSLRQKILKVTEQIKIEQTSRDGNVAEYLKLVS
  SADKQQAGRIKQVFEKKNQKSAHSIAQLQKKLEQYHRKLREIEQNGVTRSSKDISKDSLKEIHHSLKDAH
  VKSRTAPHCLESSKSSMPGVSLTPPVFVFNKSREFANLIRNKFGSADNIAHLKNSLEEFRPEASPRAYGG
  SATIVNKPKYGSDDECSSGTSGSADSNGNQSFGAGGTSTLDSQGKIAKIMEELREIKVTQTQLAEDIEAL
  KVQFKREYGFISQTLQEERYRYERLEDQLHDLTELHQHETANLKQELASAEEKVAYQAYERSRDIQEALE
  SCQTRISKLELHQQEQQTLQTDAVNAKVLLGKCINVVLAFMTVILVCVSTLAKFVSPMMKSRSHILGTFF
  AVTLLAIFCKNWDHILCAIERIIIPR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC057202
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: BC057202, AAH57202
• RefSeq Size: 3161 bp
• RefSeq ORF: 1340 bp
• Locus ID: 319880
• Cytogenetics: 10 C2