Vps4b (NM_009190) Mouse Tagged ORF Clone
CAT#: MG207089
- TrueORF®
Vps4b (tGFP-tagged) - Mouse vacuolar protein sorting 4b (yeast) (Vps4b)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_009190" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Vps4b |
Synonyms | 8030489C12Rik; Skd1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG207089 representing NM_009190
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCACGAACACCAACCTGCAGAAAGCAATAGATCTTGCAAGCAAAGCAGCCCAGGAAGACAAAG CTGGGAACTATGAGGAAGCTCTTCAACTCTACCAACATGCTGTGCAGTATTTTCTCCATGTTGTTAAATA TGAAGCACAAGGTGATAAAGCCAAGCAAAGTATCAGGGCCAAGTGTACAGAATATCTCGATAGAGCAGAA AAACTAAAGGAATATCTGAAGAAAAAGGAGAAGAAACCACAGAAGCCTGTGAAAGAGGAACAGTCAGGCC CAGTTGATGAGAAGGGGAATGACAGTGATGGGGAAGCAGAATCTGATGATCCTGAAAAAAAGAAACTGCA GAATCAACTTCAAGGTGCCATTGTTATAGAGCGACCAAATGTGAAGTGGAGTGATGTTGCTGGTCTCGAA GGAGCCAAAGAAGCTCTTAAAGAGGCTGTGATTCTGCCTATTAAGTTTCCTCATCTGTTTACAGGAAAGC GGACACCTTGGAGAGGAATCCTATTATTTGGACCACCAGGAACAGGAAAATCTTATTTGGCTAAAGCTGT TGCAACGGAAGCAAACAACTCAACATTTTTCTCAATATCTTCCTCTGACCTTGTGTCTAAGTGGCTGGGG GAAAGCGAAAAACTGGTTAAGAACTTATTCCAGCTTGCCAGAGAGAACAAGCCTTCTATCATCTTCATCG ATGAGATTGATTCTCTGTGTGGTTCCAGAAGTGAGAATGAGAGCGAGGCTGCACGGAGAATTAAGACGGA GTTCCTGGTTCAAATGCAAGGAGTTGGTGTGGACAATGATGGCATTTTGGTTCTGGGAGCTACAAATATA CCCTGGGTTCTGGATTCTGCAATTAGGCGAAGATTTGAGAAACGTATTTATATTCCTTTGCCCGAAGCCC ATGCCCGAGCAGCCATGTTTAGACTGCACTTGGGCAGCACTCAGAACAGCCTCACAGAAGCAGACTTCCA AGAACTTGGGAGGAAGACGGATGGCTATTCAGGAGCAGATATAAGTATCATTGTACGGGATGCACTTATG CAGCCTGTGAGAAAAGTTCAGTCAGCTACTCACTTCAAAAAGGTTCGTGGACCCTCAAGAGCTGATCCTA ACTGCATTGTAAATGACCTGCTGACACCCTGCTCTCCGGGAGACCCTGGGGCTATTGAAATGACATGGAT GGATGTTCCTGGAGATAAACTCTTGGAGCCAGTTGTTTCCATGTGGGATATGCTGCGGTCCCTGTCCAGC ACAAAGCCAACAGTCAATGAGCAGGACTTGTTGAAGCTGAAGAAGTTTACAGAAGATTTTGGCCAGGAAG GC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207089 representing NM_009190
Red=Cloning site Green=Tags(s) MASTNTNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAE KLKEYLKKKEKKPQKPVKEEQSGPVDEKGNDSDGEAESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLE GAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLG ESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNI PWVLDSAIRRRFEKRIYIPLPEAHARAAMFRLHLGSTQNSLTEADFQELGRKTDGYSGADISIIVRDALM QPVRKVQSATHFKKVRGPSRADPNCIVNDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMWDMLRSLSS TKPTVNEQDLLKLKKFTEDFGQEG TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_009190 |
ORF Size | 1332 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009190.1, NP_033216.1 |
RefSeq Size | 2844 bp |
RefSeq ORF | 1335 bp |
Locus ID | 20479 |
UniProt ID | P46467 |
Cytogenetics | 1 E2.1 |
Gene Summary | Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC215773 | Vps4b (untagged) - Mouse vacuolar protein sorting 4b (yeast) (Vps4b), (10ug) |
USD 503.00 |
|
MR207089 | Vps4b (Myc-DDK-tagged) - Mouse vacuolar protein sorting 4b (yeast) (Vps4b) |
USD 457.00 |
|
MR207089L3 | Lenti ORF clone of Vps4b (Myc-DDK-tagged) - Mouse vacuolar protein sorting 4b (yeast) (Vps4b) |
USD 757.00 |
|
MR207089L4 | Lenti ORF clone of Vps4b (mGFP-tagged) - Mouse vacuolar protein sorting 4b (yeast) (Vps4b) |
USD 757.00 |
{0} Product Review(s)
Be the first one to submit a review