Spock1 (BC075625) Mouse Tagged ORF Clone

CAT#: MG207043

Spock1 (tGFP-tagged) - Mouse sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 (cDNA clone MGC:99926 IMAGE:6842221), complete

Product Images

Circular map for MG207043

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Spock1
• Synonyms: Spock, testican
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207043 representing BC075625
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGGCGCGCCC
  
  ATGCCAGCGATCGCGGTGCTCGCGGCGGCCGCCGCGGCGTGGTGCTTCCTCCAAGTGGACAGCCGGCACC
  TGGACGCGCTGGCCGGTGGCGCGGCCCTCAACAACGCCAATTTCCTAGACAATGACCAGTGGCTGAGCAC
  TGTCTCCCAGTATGACCGTGACAAGTACTGGAACCGCTTCCGAGATGAAGTTGAGGATGACTATTTCAGA
  AACTGGAATCCCAACAAGCCCTTCGACCAAGCCCTGGACCCATCCAAGGACCCCTGCCTGAAGGTGAAAT
  GCAGCCCGCACAAAGTATGTGTGACCCAGGACTACCAGACGGCTCTGTGTGTCAGCCGCAAGCACCTGTT
  GCCCAGGCAGAAGAAGGGCAATGTGGCTCACAAACACTGGCTTGGACCTTCCAATCTGGTTAAGTGCAAG
  CCTTGCCCCGTGGCGCAGTCAGCGATGGTCTGCGGCTCTGACGGCCACACGTACACGTCCAAGTGCAAGT
  TGGAATTCCACGCTTGTTCTACAGGCAAAAGCCTCAACTCCCTCTGTGATGGGCCCTGTCCGTGTCTGCC
  TGAGCCTGAGCCACTGAAGCCCAAAGCAGAGAAGAGTGCCTGCACGGACAAGGAGCTGCGGAACCTCGCC
  TCCCGGCTGAAGGACTGGTTCGGGGCTCTTCATGAGGACGCCAATAGAGTCATCAAGCCTACCAGCTCTG
  ATCCAGCCCAAGGCAGGTTTGACACCAGCATCTTACCCATTTGCAAGGACTCCTTGGGTTGGATGTTCAA
  CAAGTTGGACATGAACTATGACCTGTTGCTGGACCACTCAGAGATCAATGCCATCTACCTAGACAAATAT
  GAGCCCTGCATCAAGCCTCTCTTCAACTCGTGCGACTCCTTCAAGGACGGCAAACTCTCCAACAATGAGT
  GGTGTTACTGCTTCCAGAAGCCAGCGGGTCTCCCTTGCCAGAATGAAATGAACAGAATTCAGAAGCTAAG
  CAAGGGGAAAAGCCTACTGGGGGCCTTCATCCCTCGATGTAACGAGGAGGGCTACTACAAAGCCACACAG
  TGCCACGGCAGCACGGGGCAGTGCTGGTGTGTGGATAAATATGGGAACGAGCTGGCCGGCTCCAGGAAAC
  AGGGCACTGTAAGCTGCGAAGAGGAGCAGGAAACCTCCGGGGACTTCGGCAGTGGAGGCTCCGTGGTCCT
  GCTGGATGACCTAGAGGATGAGCGGGAGCTGGGACCAAAGGACAAAGAAGGGAAGCTGAGGGTGCGCACC
  CGGGCCGTGCGGGAAGATGATGAGGATGAAGATGACGACAAAGAAGATGAGGTCGGGTACATATGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207043 representing BC075625
  Red=Cloning site Green=Tags(s)
  
  MPAIAVLAAAAAAWCFLQVDSRHLDALAGGAALNNANFLDNDQWLSTVSQYDRDKYWNRFRDEVEDDYFR
  NWNPNKPFDQALDPSKDPCLKVKCSPHKVCVTQDYQTALCVSRKHLLPRQKKGNVAHKHWLGPSNLVKCK
  PCPVAQSAMVCGSDGHTYTSKCKLEFHACSTGKSLNSLCDGPCPCLPEPEPLKPKAEKSACTDKELRNLA
  SRLKDWFGALHEDANRVIKPTSSDPAQGRFDTSILPICKDSLGWMFNKLDMNYDLLLDHSEINAIYLDKY
  EPCIKPLFNSCDSFKDGKLSNNEWCYCFQKPAGLPCQNEMNRIQKLSKGKSLLGAFIPRCNEEGYYKATQ
  CHGSTGQCWCVDKYGNELAGSRKQGTVSCEEEQETSGDFGSGGSVVLLDDLEDERELGPKDKEGKLRVRT
  RAVREDDEDEDDDKEDEVGYIW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC075625
• ORF Size: 1328 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC075625, AAH75625
• RefSeq Size: 4628 bp
• RefSeq ORF: 1328 bp
• Locus ID: 20745
• Cytogenetics: 13 B1