Traf1 (NM_009421) Mouse Tagged ORF Clone

CAT#: MG206422

Traf1 (tGFP-tagged) - Mouse Tnf receptor-associated factor 1 (Traf1)

Product Images

Circular map for MG206422

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Traf1
• Synonyms: 4732496E14Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206422 representing NM_009421
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCAGCTCAGCCCCTGATGAAAACGAGTTTCAATTTGGTTGCCCCCCTGCTCCCTGCCAGGACC
  CATCGGAGCCCAGAGTTCTCTGCTGCACAGCCTGTCTCTCTGAGAACCTGAGAGATGATGAGGATCGGAT
  CTGTCCTAAATGCAGAGCAGACAACCTCCATCCTGTGAGCCCAGGAAGCCCTCTGACTCAGGAGAAGGTT
  CACTCTGATGTAGCTGAGGCTGAAATCATGTGCCCCTTTGCAGGTGTTGGCTGTTCCTTCAAGGGGAGCC
  CACAATCCATGCAGGAGCATGAGGCTACCTCCCAGTCCTCCCACCTGTACCTGCTGCTGGCGGTCTTAAA
  GGAGTGGAAATCCTCACCAGGCTCCAACCTAGGGTCTGCACCCATGGCACTGGAGCGGAACCTGTCAGAG
  CTGCAGCTTCAGGCAGCTGTGGAAGCGACAGGGGACCTGGAGGTAGACTGCTACCGGGCACCTTGCTGTG
  AGAGCCAGGAAGAACTGGCCCTGCAGCACTTGGTGAAGGAGAAGCTGCTGGCTCAGCTGGAGGAGAAGCT
  GCGTGTGTTTGCAAACATTGTTGCTGTCCTCAACAAGGAAGTGGAGGCTTCCCACCTGGCACTGGCCGCC
  TCCATCCACCAGAGCCAGTTGGACCGAGAGTACATCCTGGGCTTGGAGCAGAGGGTGGTGGAATTACAGC
  AAACCCTGGCTCAAAAAGACCAGGTCCTGGGCAAGCTTGAGCACAGTCTGCGACTCATGGAGGAGGCATC
  CTTTGATGGTACTTTCCTGTGGAAGATCACCAATGTCACCAAGCGGTGCCACGAGTCAGTGTGTGGCCGG
  ACTGTCAGCCTCTTCTCTCCAGCTTTCTACACTGCCAAGTATGGTTACAAGTTGTGCCTGCGCTTGTACC
  TGAACGGGGATGGCTCAGGCAAGAAGACCCACCTGTCCCTCTTCATCGTGATCATGAGAGGAGAATACGA
  TGCTCTCCTGCCCTGGCCTTTCAGGAACAAGGTCACCTTTATGCTACTTGACCAGAACAACCGAGAGCAT
  GCTATTGATGCCTTCCGGCCTGACCTGAGCTCAGCCTCCTTCCAGCGGCCACAGAGTGAGACCAACGTGG
  CCAGCGGCTGCCCGCTCTTCTTCCCCCTCAGCCAGCTGCAGTCACCCAAGCACGCCTACGTCAAAGATGG
  CACAATGTTCCTCAAATGCATTGTGGACACTAGTGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206422 representing NM_009421
  Red=Cloning site Green=Tags(s)
  
  MASSSAPDENEFQFGCPPAPCQDPSEPRVLCCTACLSENLRDDEDRICPKCRADNLHPVSPGSPLTQEKV
  HSDVAEAEIMCPFAGVGCSFKGSPQSMQEHEATSQSSHLYLLLAVLKEWKSSPGSNLGSAPMALERNLSE
  LQLQAAVEATGDLEVDCYRAPCCESQEELALQHLVKEKLLAQLEEKLRVFANIVAVLNKEVEASHLALAA
  SIHQSQLDREYILGLEQRVVELQQTLAQKDQVLGKLEHSLRLMEEASFDGTFLWKITNVTKRCHESVCGR
  TVSLFSPAFYTAKYGYKLCLRLYLNGDGSGKKTHLSLFIVIMRGEYDALLPWPFRNKVTFMLLDQNNREH
  AIDAFRPDLSSASFQRPQSETNVASGCPLFFPLSQLQSPKHAYVKDGTMFLKCIVDTSA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009421
• ORF Size: 1227 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009421.3, NP_033447.2
• RefSeq Size: 2325 bp
• RefSeq ORF: 1230 bp
• Locus ID: 22029
• UniProt ID: P39428
• Cytogenetics: 2 B
• Gene Summary: Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2 (By similarity).[UniProtKB/Swiss-Prot Function]