Cers6 (NM_172856) Mouse Tagged ORF Clone

CAT#: MG205998

Cers6 (tGFP-tagged) - Mouse longevity assurance homolog 6 (S. cerevisiae) (Lass6)

Product Images

Circular map for MG205998

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cers6
• Synonyms: 4732462C07Rik; AW544719; Lass6; T1L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205998 representing NM_172856
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGGATCTTAGCCTGGTTCTGGAACGAGCGGTTTTGGCTTCCGCACAATGTCACCTGGGCAGACC
  TGAAGAACACGGAGGAAGCCACCTTTCCGCAGGCGGAGGACCTCTACCTTGCCTTCCCCTTGGCCTTCTG
  CATCTTCATGGTGCGGCTCATCTTCGAGAGATTCATAGCCAAACCATGTGCCATAGCCCTCAACATCCAA
  GCCAATGGACCACAAACTGCCCAGCCAAATGCCATTCTGGAAAAGGTTTTCACTGCTATAACAAAGCATC
  CTGATGAAAAGAGATTAGAAGGGCTCTCCAAGCAGCTGGACTGGGATGTTCGGAGCATTCAACGCTGGTT
  TCGACAAAGACGCAACCAGGAGAAACCCAGCACTCTGACCAGGTTCTGTGAGAGCATGTGGAGATTTTCC
  TTTTACCTCTATGTATTTAGCTACGGAGTCCGGTTCTTGAAACAGACCCCTTGGTTGTGGAATACAAGAC
  ACTGCTGGTATAACTACCCTTACCAGCCACTCACAGCTGACCTTCACTACTATTACATCCTGGAGCTGTC
  ATTTTATTGGTCTTTAATGGTTTCCCAGTTCACAGATATCAAAAGGAAGGACTTCGGCATTATGTTCCTG
  CACCACCTTGCAACAATTTTCTTAATAACCTTTTCATATGTCAACAACATGGCCCGAGTAGGAACCCTGG
  TCCTCTGTCTTCACGACTCAGCCGATGCTCTGCTAGAGGCTGCCAAAATGGCAAATTACGCCAAATTTCA
  GAAAATGTGTGATCTCCTGTTTGTCATGTTTGCCGTGGTTTTTATCACCACACGGCTGGGCATATTTCCT
  CTCTGGGTGTTGAATACCACATTATTTGAAAGTTGGGAGATCGTCGGACCCTACCCTTCCTGGTGGGTTT
  TTAACCTGCTGCTCTTGTTACTACAAGGGTTGAACTGCTTCTGGTCTTACCTGATTGTAAAAATAGCTTG
  CAAAACTGTTTCAAAAGGCAAGGTATCCAAGGATGACCGGAGTGACATTGAATCTAGCTCAGATGATGAG
  GACTCAGAGCCTCCAGGGAAGAAACCACACTCTTCAACAACCACCAACGGAACCAGCGGTACCAATGGGT
  ACCTCCTCACTGGTCCTTGTTCCGTGGATGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205998 representing NM_172856
  Red=Cloning site Green=Tags(s)
  
  MAGILAWFWNERFWLPHNVTWADLKNTEEATFPQAEDLYLAFPLAFCIFMVRLIFERFIAKPCAIALNIQ
  ANGPQTAQPNAILEKVFTAITKHPDEKRLEGLSKQLDWDVRSIQRWFRQRRNQEKPSTLTRFCESMWRFS
  FYLYVFSYGVRFLKQTPWLWNTRHCWYNYPYQPLTADLHYYYILELSFYWSLMVSQFTDIKRKDFGIMFL
  HHLATIFLITFSYVNNMARVGTLVLCLHDSADALLEAAKMANYAKFQKMCDLLFVMFAVVFITTRLGIFP
  LWVLNTTLFESWEIVGPYPSWWVFNLLLLLLQGLNCFWSYLIVKIACKTVSKGKVSKDDRSDIESSSDDE
  DSEPPGKKPHSSTTTNGTSGTNGYLLTGPCSVDD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172856
• ORF Size: 1152 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172856.4
• RefSeq Size: 3931 bp
• RefSeq ORF: 1155 bp
• Locus ID: 241447
• UniProt ID: Q8C172
• Cytogenetics: 2 C2
• Gene Summary: May be involved in sphingolipid synthesis or its regulation.[UniProtKB/Swiss-Prot Function]