Rab3il1 (NM_144538) Mouse Tagged ORF Clone

CAT#: MG205981

Rab3il1 (tGFP-tagged) - Mouse RAB3A interacting protein (rabin3)-like 1 (Rab3il1)

Product Images

Circular map for MG205981

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rab3il1
• Synonyms: 1200014K04Rik; AI115013; C76746; Rab3ail1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205981 representing NM_144538
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAGCGGCCAACCCCAACAAGACGAGGGCCTCCCTGTGGGCCTCTCTGCTATCCCAGTCCCCTGGA
  AAAACCTAGGCCCCAGCAAAAGTAACAGGGAGTCCTCAGGAGGCCTGGTAGAAGCCTCCACTTCCTGGGA
  GGAGGCCCAGGGTGAGGAACACCCGGCACCTGCTCCACTGGATGTGTCACGCCTGCGCAGTTCCTCCATG
  GAGATCCGAGAGAAGGGCTCCGAGTTCCTAAAGGAGGAGCTATACAAAGCCCAGAAGGAGCTGAAGCTAA
  AGGATGAAGAGTGTGAACGGCTTTGCAAAGTACGTGCACAGCTGGAGCAGGAGCTTGAGGAGCTGACAGC
  CAGCCTGTTTGAGGAAGCTCACAAGATGGTTCGGGAAGCCAACATGAAGCAGGCGACATCGGAAAAGCAG
  TTGAAGGAGGCTTGGGGCAAGATCGACATGCTACAGGCAGAGGTGACAGCCTTGAAGACATTGGTCATCA
  CATCCACACCAGCCTCTCCCAACCGTGAGCTCCACCCACAGCTGCTGAGCCCCACCAAAGCTGGACCCCG
  AAAGGGCCACTCACGTCAAAAGAGCACCAGCAGCTCCCTCTGCCCTGTTGTGTGCCCCACTGCAGGGCAT
  ATCCCTACCCCTGACAAAGAAGGCAAGGAGGTGGATACGACGCTGTTTGCAGAGTTCCAAGCTTGGAGAG
  CTTCACCTACCCTGGATAAGAGCTGCCCCTTCCTAGAAAGGGTGTACCGGGAGGACGTGGGCCCTTGCCT
  TGACTTCACAGTGCAGGAGCTCTCAGCTCTGGTTCGGACTGCTGTGGAGGACAACACACTCACCATTGAG
  CCTGTGGCTTCACAGACACTGCCCGCTGTGAAGGTGCCCACTGTTGAGTGTAACAACACCAACACATGTG
  CCCTGAGTGGCCTGGCACGTACCTGCCACCATCGAATCCGCCTGGGGGACTCTGACAGCCACTATTACAT
  CTCACCGTCCTCCCGGGCCAGGATCACCGCAGTATGCAACTTCTTCACCTATATTCGCTACATCCAGCAA
  GGTCTGGTTCGGCAGGATGCTGAGCCGATGTTCTGGGAGATCATGAGGCTTCGGAAGGGCATGTCACTAG
  CCAAGCTTGGCTTCTTCCCCCAGGAGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205981 representing NM_144538
  Red=Cloning site Green=Tags(s)
  
  MWSGQPQQDEGLPVGLSAIPVPWKNLGPSKSNRESSGGLVEASTSWEEAQGEEHPAPAPLDVSRLRSSSM
  EIREKGSEFLKEELYKAQKELKLKDEECERLCKVRAQLEQELEELTASLFEEAHKMVREANMKQATSEKQ
  LKEAWGKIDMLQAEVTALKTLVITSTPASPNRELHPQLLSPTKAGPRKGHSRQKSTSSSLCPVVCPTAGH
  IPTPDKEGKEVDTTLFAEFQAWRASPTLDKSCPFLERVYREDVGPCLDFTVQELSALVRTAVEDNTLTIE
  PVASQTLPAVKVPTVECNNTNTCALSGLARTCHHRIRLGDSDSHYYISPSSRARITAVCNFFTYIRYIQQ
  GLVRQDAEPMFWEIMRLRKGMSLAKLGFFPQEA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144538
• ORF Size: 1149 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144538.3
• RefSeq Size: 2320 bp
• RefSeq ORF: 1152 bp
• Locus ID: 74760
• UniProt ID: Q8VDV3
• Cytogenetics: 19 A
• Gene Summary: Guanine nucleotide exchange factor (GEF) which may activate RAB3A, a GTPase that regulates synaptic vesicle exocytosis. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. May also activates RAB8A and RAB8B (By similarity).[UniProtKB/Swiss-Prot Function]