Actg2 (BC002042) Mouse Tagged ORF Clone
CAT#: MG205841
- TrueORF®
Actg2 (tGFP-tagged) - Mouse actin, gamma 2, smooth muscle, enteric (cDNA clone MGC:6022 IMAGE:3591323)
"BC002042" in other vectors (1)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Actg2 |
Synonyms | SMGA, ACTA3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205841 representing BC002042
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGAAGAAGAGACCACCGCCCTTGTGTGTGACAATGGCTCTGGCCTGTGCAAGGCAGGCTTTGCAG GAGATGATGCCCCCAGGGCTGTCTTCCCCTCCATTGTGGGCCGCCCTAGACATCAGGGTGTGATGGTGGG AATGGGCCAGAAAGACAGCTATGTGGGGGACGAGGCTCAGAGCAAGCGTGGGATCCTAACTCTCAAGTAC CCTATTGAACATGGCATCATCACCAACTGGGATGACATGGAGAAGATCTGGCACCACTCCTTCTACAATG AGCTTCGAGTAGCACCAGAAGAGCACCCCACCCTGCTCACAGAGGCCCCCCTAAACCCCAAAGCAAACAG GGAGAAGATGACCCAGATCATGTTCGAAACCTTCAATGTCCCTGCCATGTATGTTGCTATTCAGGCTGTG CTCTCACTCTATGCATCCGGCCGTACCACAGGCATCGTCCTGGATTCGGGGGATGGCGTCACCCACAATG TCCCCATCTATGAGGGCTATGCACTGCCCCATGCCATCATGCGTCTTGACCTGGCTGGACGGGATCTCAC AGACTACCTCATGAAGATTCTCACAGAAAGAGGCTATTCCTTTGTGACCACAGCTGAGAGAGAAATTGTA CGAGACATCAAGGAGAAGCTGTGCTATGTAGCCCTGGATTTCGAGAATGAGATGGCCACAGCAGCTTCAT CTTCTTCCCTGGAGAAAAGCTACGAGTTGCCTGATGGGCAGGTCATCACTATTGGCAACGAACGCTTCCG CTGCCCGGAGACCCTCTTCCAGCCTTCCTTCATTGGCATGGAGTCAGCTGGAATTCATGAAACAACATAC AATTCCATCATGAAGTGTGACATTGACATCCGCAAAGATTTGTATGCTAACAATGTCCTCTCTGGGGGCA CTACCATGTACCCTGGCATTGCTGACAGGATGCAGAAGGAAATCACAGCCTTGGCTCCCAGCACCATGAA GATCAAGATTATCGCTCCTCCTGAGCGGAAGTACTCAGTCTGGATTGGCGGCTCCATCCTGGCCTCTCTC TCCACCTTCCAGCAAATGTGGATCAGCAAGCCAGAGTATGATGAGGCAGGGCCCTCCATTGTCCACAGGA AATGCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205841 representing BC002042
Red=Cloning site Green=Tags(s) MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKY PIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAV LSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIV RDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTY NSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASL STFQQMWISKPEYDEAGPSIVHRKCF TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC002042 |
ORF Size | 1130 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC002042, AAH02042 |
RefSeq Size | 1319 bp |
RefSeq ORF | 1130 bp |
Locus ID | 11468 |
Cytogenetics | 6 35.94 cM |
Gene Summary | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200368 | Actg2 (untagged) - Mouse actin, gamma 2, smooth muscle, enteric (cDNA clone MGC:6022 IMAGE:3591323), (10ug) |
USD 457.00 |
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