Macroh2a2 (NM_207000) Mouse Tagged ORF Clone

CAT#: MG205746

H2afy2 (tGFP-tagged) - Mouse H2A histone family, member Y2 (H2afy2)

Product Images

Circular map for MG205746

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Macroh2a2
• Synonyms: H2afy; H2afy2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205746 representing NM_207000
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGCCGGAGCGGGAAGAAGAAAATGTCCAAGCTGTCCCGGTCAGCCAGGGCCGGCGTCATCTTTC
  CAGTAGGCCGGTTGATGCGCTACTTGAAAAAGGGAACCTTCAAGTACAGGATCAGCGTAGGTGCTCCCGT
  CTACATGGCTGCGGTCATTGAGTACCTAGCAGCGGAAATCCTGGAGTTGGCTGGGAACGCTGCGAGGGAC
  AACAAGAAGGCACGGATAGCCCCCAGACACATCCTGCTGGCCGTTGCCAACGACGAGGAACTCAACCAGC
  TACTGAAAGGAGTGACTATCGCCAGTGGGGGTGTCCTGCCGAGAATCCACCCCGAACTGCTGGCCAAGAA
  GCGAGGGACCAAAGGCAAGTCAGAGACGATCCTCTCCCCACCCCCAGAGAAAAGAGGAAGGAAGGCCGCA
  TCAGGCAAGAAGGGGGGTAAGAAATCCAAGGCCACCAAGCCACGGACGTCCAAGAAGTCTAAAGCAAAGG
  ACAGCGATAAAGAAGGAACATCAAATTCCACCTCGGAGGATGGGCCGGGAGATGGCTTCACCATCTTGTC
  TTCGAAGAGCCTCGTTCTGGGGCAGAAGCTATCCCTGACCCAGAGTGACATCAGCCATATTGGCTCCATG
  AGGGTGGAGGGCATCGTCCACCCAACCACAGCCGAGATCGACCTCAAGGAAGAGATAGGGAAAGCCTTGG
  AAAAGGCTGGGGGTAAAGAGTTCTTGGAAACAGTGAAGGAGCTTCGCAAGTCCCAAGGCCCTTTGGAAGT
  CGCTGAAGCTGCCGTCAGCCAATCCAGTGGACTCGCAGCCAAATTTGTCATCCACTGTCACATCCCCCAG
  TGGGGCTCCGACAAATGTGAAGAACAGCTGGAAGAGACCATCAAAAACTGCCTGTCTGCAGCAGAGGACA
  AGAAGCTTAAATCCGTCGCCTTCCCACCGTTCCCCAGTGGCAGAAACTGCTTCCCCAAACAGACGGCCGC
  CCAGGTGACCCTCAAGGCCATCTCGGCTCACTTCGACGACTCGAGCTCGTCCTCGCTGAAGAATGTGTAC
  TTCCTGCTCTTCGACAGCGAGAGCATCGGCATCTACGTGCAGGAGATGGCCAAACTGGACACCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205746 representing NM_207000
  Red=Cloning site Green=Tags(s)
  
  MSGRSGKKKMSKLSRSARAGVIFPVGRLMRYLKKGTFKYRISVGAPVYMAAVIEYLAAEILELAGNAARD
  NKKARIAPRHILLAVANDEELNQLLKGVTIASGGVLPRIHPELLAKKRGTKGKSETILSPPPEKRGRKAA
  SGKKGGKKSKATKPRTSKKSKAKDSDKEGTSNSTSEDGPGDGFTILSSKSLVLGQKLSLTQSDISHIGSM
  RVEGIVHPTTAEIDLKEEIGKALEKAGGKEFLETVKELRKSQGPLEVAEAAVSQSSGLAAKFVIHCHIPQ
  WGSDKCEEQLEETIKNCLSAAEDKKLKSVAFPPFPSGRNCFPKQTAAQVTLKAISAHFDDSSSSSLKNVY
  FLLFDSESIGIYVQEMAKLDTK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207000
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207000.2, NP_996883.1
• RefSeq Size: 1910 bp
• RefSeq ORF: 1119 bp
• Locus ID: 404634
• UniProt ID: Q8CCK0
• Cytogenetics: 10 B4
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and may participate in stable X chromosome inactivation. [provided by RefSeq, Nov 2015]