Timd4 (BC032879) Mouse Tagged ORF Clone

CAT#: MG205549

Timd4 (tGFP-tagged) - Mouse T-cell immunoglobulin and mucin domain containing 4 (cDNA clone MGC:41117 IMAGE:1195144)

Product Images

Circular map for MG205549

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Timd4
• Synonyms: Tim4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205549 representing BC032879
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTTGATGATTCAAGGCCATCGTTTATTAAAACTAATTACCTCGTGCCGAATTCGGCACGAGGGGC
  TTCTCATCCTCTGGCTGGTGACGGAGCTCTGGTGGCTTTATCTGACACCAGCTGCCTCAGAGGATACAAT
  AATAGGGTTTTTGGGCCAGCCGGTGACTTTGCCTTGTCATTACCTCTCGTGGTCCCAGAGCCGCAACAGT
  ATGTGCTGGGGCAAAGGTTCATGTCCCAATTCCAAGTGCAATGCAGAGCTTCTCCGTACAGATGGAACAA
  GAATCATCTCCAGGAAGTCAACAAAATATACACTTTTGGGGAAGGTCCAGTTTGGTGAAGTGTCCTTGAC
  CATCTCAAACACCAATCGAGGTGACAGTGGGGTGTACTGCTGCCGTATAGAGGTGCCTGGCTGGTTCAAT
  GATGTCAAGAAGAATGTGCGCTTGGAGCTGAGGAGAGCCACAACAACCAAAAAACCAACAACAACCACCC
  GGCCAACCACCACCCCTTATGTGACCACCACCACCCCAGAGCTGCTTCCAACAACAGTCATGACCACATC
  TGTTCTCCCAACCACCACACCACCCCAGACACTAGCCACCACTGCCTTCAGTACAGCAGTGACCACGTGC
  CCCTCAACAACACCTGGCTCCTTCTCACAAGAAACCACAAAAGGGTCCGCCTTCACTACAGAATCAGAAA
  CTCTGCCTGCATCCAATCACTCTCAAAGAAGCATGATGACCATATCTACAGACATAGCCGTACTCAGGCC
  CACAGGCTCTAACCCTGGGATTCTCCCATCCACTTCACAGCTGACGACACAGAAAACAACATTAACAACA
  AGTGAGTCTTTGCAGAAGACAACTAAATCACATCAGATCAACAGCAGACAGACCATCTTGATCATTGCCT
  GCTGTGTGGGATTTGTGCTAATGGTGTTATTGTTTCTGGCGTTTCTCCTTCGAGGGAAAGTCACAGGAGC
  CAACTGTTTGCAGAGACACAAGAGGCCAGACAACACTGAAGATAGTGACAGCGTCCTCAATGACATGTCA
  CACGGGAGGGATGATGAAGACGGGATCTTCACTCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205549 representing BC032879
  Red=Cloning site Green=Tags(s)
  
  MNLMIQGHRLLKLITSCRIRHEGLLILWLVTELWWLYLTPAASEDTIIGFLGQPVTLPCHYLSWSQSRNS
  MCWGKGSCPNSKCNAELLRTDGTRIISRKSTKYTLLGKVQFGEVSLTISNTNRGDSGVYCCRIEVPGWFN
  DVKKNVRLELRRATTTKKPTTTTRPTTTPYVTTTTPELLPTTVMTTSVLPTTTPPQTLATTAFSTAVTTC
  PSTTPGSFSQETTKGSAFTTESETLPASNHSQRSMMTISTDIAVLRPTGSNPGILPSTSQLTTQKTTLTT
  SESLQKTTKSHQINSRQTILIIACCVGFVLMVLLFLAFLLRGKVTGANCLQRHKRPDNTEDSDSVLNDMS
  HGRDDEDGIFTL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC032879
• ORF Size: 1088 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC032879
• RefSeq Size: 2331 bp
• RefSeq ORF: 1088 bp
• Locus ID: 276891
• Cytogenetics: 11 B1.1
• Gene Summary: Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1.[UniProtKB/Swiss-Prot Function]