Impad1 (NM_177730) Mouse Tagged ORF Clone

CAT#: MG205434

Impad1 (tGFP-tagged) - Mouse inositol monophosphatase domain containing 1 (Impad1)

Product Images

Circular map for MG205434

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Impad1
• Synonyms: 1110001C20Rik; AA408880; AI451589; AL022796; B230207P20; gPAPP; Jaws
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205434 representing NM_177730
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCATGGGCATCCGCCTGTCCCCGCTGGGGGTGGCCGTATTTTTCCTGCTGGGGCTCGGGGTGC
  TCTACCACCTCTACTCGGGCTTCCTGGCCGGCCGCTTCAGCCTCTTCGGCCTGGGCAGCGAGCCGGCGGC
  AGGGGAGGCCGAGGTGGCGTCCGACGGGGGCACGGTGGACCTGCGCGAGATGCTGGCCGTGGCCGTGCTG
  GCGGCCGAGCGCGGCGGCGACGAGGTGAGGCGGGTCCGCGAGAGCAATGTCCTTCACGAGAAGTCCAAGG
  GGAAGACGCGCGAGGGCGCCGATGATAAGATGACCAGCGGCGACGTACTGTCCAACCGCAAGATGTTCTA
  CCTGCTCAAGACGGCCTTCCCCAACGTGCAGATAAATACTGAGGAACATGTGGATGCCTCTGACAAGGAG
  GTCATTGTATGGAACCGGAAGATTCCTGAGGACATCCTGAAGGAAATCGCTGCTCCTAAGGAGGTACCAG
  CAGAAAGTGTGACAGTTTGGATTGACCCACTTGATGCTACACAGGAATACACAGAGGATCTTCGAAAGTA
  TGTCACTACCATGGTGTGTGTAGCTGTGAATGGCAAACCTGTGTTAGGAGTTATTCATAAGCCATTTTCT
  GAATATACAGCTTGGGCAATGGTAGATGGTGGTTCAAATGTGAAAGCCCGTTCTTCCTACAATGAGAAGA
  CCCCAAAGATCATTGTGTCTCGCTCTCATGCAGGAATGGTCAAACAGGTTGCTCTTCAGACGTTTGGAAA
  CCAGACTTCAATTATCCCAGCTGGGGGTGCTGGTTATAAAGTTTTAGCACTCTTGGATGTGCCTGATATG
  ACTCAAGAAAAAGCAGATCTATATATTCATGTGACATACATCAAAAAGTGGGATATATGTGCTGGCAATG
  CCATCTTAAAAGCCCTTGGTGGCCATATGACCACTCTGAATGGAGAAGAGATCAGTTACACTGGGTCTGA
  TGGCATTGAAGGGGGACTTCTTGCTAGCATCAGAATGAACCACCAAGCTCTGGTCAGAAAACTTCCGGAT
  TTAGAAAAGTCAGGACAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205434 representing NM_177730
  Red=Cloning site Green=Tags(s)
  
  MAPMGIRLSPLGVAVFFLLGLGVLYHLYSGFLAGRFSLFGLGSEPAAGEAEVASDGGTVDLREMLAVAVL
  AAERGGDEVRRVRESNVLHEKSKGKTREGADDKMTSGDVLSNRKMFYLLKTAFPNVQINTEEHVDASDKE
  VIVWNRKIPEDILKEIAAPKEVPAESVTVWIDPLDATQEYTEDLRKYVTTMVCVAVNGKPVLGVIHKPFS
  EYTAWAMVDGGSNVKARSSYNEKTPKIIVSRSHAGMVKQVALQTFGNQTSIIPAGGAGYKVLALLDVPDM
  TQEKADLYIHVTYIKKWDICAGNAILKALGGHMTTLNGEEISYTGSDGIEGGLLASIRMNHQALVRKLPD
  LEKSGH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177730
• ORF Size: 1068 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177730.4
• RefSeq Size: 4636 bp
• RefSeq ORF: 1071 bp
• Locus ID: 242291
• UniProt ID: Q80V26
• Cytogenetics: 4 A1
• Gene Summary: May play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3',5'-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation.[UniProtKB/Swiss-Prot Function]