Ctss (NM_021281) Mouse Tagged ORF Clone

CAT#: MG205097

Ctss (tGFP-tagged) - Mouse cathepsin S (Ctss)

Product Images

Circular map for MG205097

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ctss
• Synonyms: Cats
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205097 representing NM_021281
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGCTCCTGGCCACGCTGCCATAAGATGGCTGTTTTGGATGCCCCTGGTGTGCTCTGTGGCAATGG
  AGCAACTGCAGAGAGACCCTACCCTGGACTACCACTGGGATCTCTGGAAGAAAACCCATGAGAAAGAATA
  CAAGGATAAGAATGAAGAAGAAGTACGGCGTCTCATCTGGGAAAAGAATCTGAAGTTTATAATGATTCAC
  AATCTCGAATATTCTATGGGAATGCATACCTACCAAGTGGGCATGAACGATATGGGAGACATGACCAATG
  AAGAAATCTTGTGTCGGATGGGTGCTCTGAGAATTCCCCGACAGTCTCCGAAGACTGTCACTTTCAGGTC
  ATACTCTAATCGGACATTGCCTGACACTGTGGACTGGAGAGAGAAGGGCTGCGTCACTGAGGTGAAATAC
  CAGGGTTCTTGTGGTGCCTGTTGGGCTTTCAGTGCTGTGGGGGCCCTTGAAGGGCAGCTGAAGCTGAAAA
  CGGGGAAGCTGATATCCCTCAGTGCTCAGAACCTGGTGGACTGCTCAAATGAAGAAAAGTATGGGAATAA
  AGGCTGTGGAGGCGGCTACATGACCGAAGCTTTCCAGTACATCATTGATAATGGGGGCATAGAGGCAGAC
  GCTTCCTATCCCTACAAAGCCATGGATGAAAAGTGTCACTATAACTCAAAAAATCGAGCTGCTACGTGTT
  CAAGGTACATTCAGCTCCCCTTTGGTGACGAGGATGCCCTGAAAGAAGCAGTGGCCACTAAAGGGCCTGT
  CTCTGTGGGCATCGACGCCAGCCATTCCTCCTTCTTCTTCTACAAAAGCGGTGTCTATGACGACCCCTCC
  TGTACGGGCAATGTGAATCATGGTGTTCTTGTGGTTGGCTATGGGACTCTTGATGGCAAAGATTACTGGC
  TTGTGAAAAACAGTTGGGGCCTTAACTTTGGTGATCAAGGATACATTCGGATGGCAAGAAATAATAAAAA
  TCACTGCGGAATTGCTAGTGATTGCTCTTACCCAGAAATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205097 representing NM_021281
  Red=Cloning site Green=Tags(s)
  
  MRAPGHAAIRWLFWMPLVCSVAMEQLQRDPTLDYHWDLWKKTHEKEYKDKNEEEVRRLIWEKNLKFIMIH
  NLEYSMGMHTYQVGMNDMGDMTNEEILCRMGALRIPRQSPKTVTFRSYSNRTLPDTVDWREKGCVTEVKY
  QGSCGACWAFSAVGALEGQLKLKTGKLISLSAQNLVDCSNEEKYGNKGCGGGYMTEAFQYIIDNGGIEAD
  ASYPYKAMDEKCHYNSKNRAATCSRYIQLPFGDEDALKEAVATKGPVSVGIDASHSSFFFYKSGVYDDPS
  CTGNVNHGVLVVGYGTLDGKDYWLVKNSWGLNFGDQGYIRMARNNKNHCGIASDCSYPEI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021281
• ORF Size: 1020 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021281.1, NP_067256.1
• RefSeq Size: 1305 bp
• RefSeq ORF: 1023 bp
• Locus ID: 13040
• UniProt ID: O70370
• Cytogenetics: 3 40.74 cM
• Gene Summary: This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. Alternative splicing results in multiple transcript variants, which encode preproproteins that are proteolytically processed to generate mature protein products. This enzyme is secreted by antigen-presenting cells during inflammation and may induce pain and itch via activation of G-protein coupled receptors. Homozygous knockout mice for this gene exhibit impaired wound healing, reduced tumorigenesis in a pancreatic cancer model, and reduced pathogenesis in a myasthenia gravis model. [provided by RefSeq, Aug 2015]