Ctss (NM_021281) Mouse Tagged ORF Clone
CAT#: MG205097
- TrueORF®
Ctss (tGFP-tagged) - Mouse cathepsin S (Ctss)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_021281" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Ctss |
Synonyms | Cats |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205097 representing NM_021281
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGCTCCTGGCCACGCTGCCATAAGATGGCTGTTTTGGATGCCCCTGGTGTGCTCTGTGGCAATGG AGCAACTGCAGAGAGACCCTACCCTGGACTACCACTGGGATCTCTGGAAGAAAACCCATGAGAAAGAATA CAAGGATAAGAATGAAGAAGAAGTACGGCGTCTCATCTGGGAAAAGAATCTGAAGTTTATAATGATTCAC AATCTCGAATATTCTATGGGAATGCATACCTACCAAGTGGGCATGAACGATATGGGAGACATGACCAATG AAGAAATCTTGTGTCGGATGGGTGCTCTGAGAATTCCCCGACAGTCTCCGAAGACTGTCACTTTCAGGTC ATACTCTAATCGGACATTGCCTGACACTGTGGACTGGAGAGAGAAGGGCTGCGTCACTGAGGTGAAATAC CAGGGTTCTTGTGGTGCCTGTTGGGCTTTCAGTGCTGTGGGGGCCCTTGAAGGGCAGCTGAAGCTGAAAA CGGGGAAGCTGATATCCCTCAGTGCTCAGAACCTGGTGGACTGCTCAAATGAAGAAAAGTATGGGAATAA AGGCTGTGGAGGCGGCTACATGACCGAAGCTTTCCAGTACATCATTGATAATGGGGGCATAGAGGCAGAC GCTTCCTATCCCTACAAAGCCATGGATGAAAAGTGTCACTATAACTCAAAAAATCGAGCTGCTACGTGTT CAAGGTACATTCAGCTCCCCTTTGGTGACGAGGATGCCCTGAAAGAAGCAGTGGCCACTAAAGGGCCTGT CTCTGTGGGCATCGACGCCAGCCATTCCTCCTTCTTCTTCTACAAAAGCGGTGTCTATGACGACCCCTCC TGTACGGGCAATGTGAATCATGGTGTTCTTGTGGTTGGCTATGGGACTCTTGATGGCAAAGATTACTGGC TTGTGAAAAACAGTTGGGGCCTTAACTTTGGTGATCAAGGATACATTCGGATGGCAAGAAATAATAAAAA TCACTGCGGAATTGCTAGTGATTGCTCTTACCCAGAAATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205097 representing NM_021281
Red=Cloning site Green=Tags(s) MRAPGHAAIRWLFWMPLVCSVAMEQLQRDPTLDYHWDLWKKTHEKEYKDKNEEEVRRLIWEKNLKFIMIH NLEYSMGMHTYQVGMNDMGDMTNEEILCRMGALRIPRQSPKTVTFRSYSNRTLPDTVDWREKGCVTEVKY QGSCGACWAFSAVGALEGQLKLKTGKLISLSAQNLVDCSNEEKYGNKGCGGGYMTEAFQYIIDNGGIEAD ASYPYKAMDEKCHYNSKNRAATCSRYIQLPFGDEDALKEAVATKGPVSVGIDASHSSFFFYKSGVYDDPS CTGNVNHGVLVVGYGTLDGKDYWLVKNSWGLNFGDQGYIRMARNNKNHCGIASDCSYPEI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021281 |
ORF Size | 1020 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021281.1, NP_067256.1 |
RefSeq Size | 1305 bp |
RefSeq ORF | 1023 bp |
Locus ID | 13040 |
UniProt ID | O70370 |
Cytogenetics | 3 40.74 cM |
Gene Summary | This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. Alternative splicing results in multiple transcript variants, which encode preproproteins that are proteolytically processed to generate mature protein products. This enzyme is secreted by antigen-presenting cells during inflammation and may induce pain and itch via activation of G-protein coupled receptors. Homozygous knockout mice for this gene exhibit impaired wound healing, reduced tumorigenesis in a pancreatic cancer model, and reduced pathogenesis in a myasthenia gravis model. [provided by RefSeq, Aug 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205662 | Ctss (untagged) - Mouse cathepsin S (Ctss), (10ug) |
USD 457.00 |
|
MR205097 | Ctss (Myc-DDK-tagged) - Mouse cathepsin S (Ctss) |
USD 457.00 |
|
MR205097L3 | Lenti ORF clone of Ctss (Myc-DDK-tagged) - Mouse cathepsin S (Ctss) |
USD 757.00 |
|
MR205097L4 | Lenti ORF clone of Ctss (mGFP-tagged) - Mouse cathepsin S (Ctss) |
USD 757.00 |
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