Fbp1 (NM_019395) Mouse Tagged ORF Clone
CAT#: MG205054
- TrueORF®
Fbp1 (tGFP-tagged) - Mouse fructose bisphosphatase 1 (Fbp1)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_019395" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Fbp1 |
Synonyms | Fbp-2; Fbp2; Fbp3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205054 representing NM_019395
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAACCATGCGCCCTTCGAAACGGATATCAGCACCCTGACCCGCTTCGTCATGGAGCAGGGCAGGA AGGCTCAGGGCACGGGGGAGTTGACCCAGCTGCTGAATTCGCTCTGCACCGCGATCAAAGCCATCTCGTC TGCGGTGCGCCAGGCGGGCATCGCACAGCTCTATGGTATCGCTGGCTCAACCAATGTGACTGGGGATCAA GTAAAGAAGCTGGACATACTTTCCAATGACCTGGTGATCAATATGCTGAAGTCGTCCTACGCTACCTGTG TTCTTGTGTCTGAAGAAAACACAAATGCCATCATAATCGAACCTGAGAAGAGGGGCAAATATGTTGTCTG TTTCGATCCCCTTGATGGCTCATCCAACATTGACTGCCTTGTGTCCATCGGAACCATTTTTGGCATTTAC AGAAAGAAAAGTACTGATGAGCCTTCTGAGAAGGATGCTCTGCAGCCCGGCCGGGACCTGGTGGCAGCCG GGTATGCGCTCTATGGCAGTGCCACCATGTTGGTCCTTGCCATGGATTGTGGTGTCAACTGCTTCATGCT GGACCCGTCCATTGGAGAATTCATTATGGTGGACAGGGACGTGAAGATGAAGAAGAAAGGTAACATCTAC AGCCTTAATGAGGGTTATGCCAAGGACTTTGACCCTGCCATCAATGAGTATCTCCAGAGGAAAAAGTTCC CTCCGGATGGTTCAGCCCCCTATGGTGCCCGGTATGTGGGGTCCATGGTGGCTGATATTCACCGCACTCT GGTATATGGAGGGATCTTTTTATACCCCGCCAACAAGAAAAGCCCAAGTGGAAAGCTGCGGCTGCTGTAT GAGTGCAACCCCATAGCTTATGTCATGGAGAAGGCCGGTGGGCTCGCCACCACGGGGGACAAAGATATAT TAGACATCGTTCCCACCGAGATCCACCAGAAGGCACCAGTCGTCATGGGGTCCTCTGAAGATGTGCAGGA GTTCCTGGAGATCTACAGGAAGCACAAAGCCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205054 representing NM_019395
Red=Cloning site Green=Tags(s) MANHAPFETDISTLTRFVMEQGRKAQGTGELTQLLNSLCTAIKAISSAVRQAGIAQLYGIAGSTNVTGDQ VKKLDILSNDLVINMLKSSYATCVLVSEENTNAIIIEPEKRGKYVVCFDPLDGSSNIDCLVSIGTIFGIY RKKSTDEPSEKDALQPGRDLVAAGYALYGSATMLVLAMDCGVNCFMLDPSIGEFIMVDRDVKMKKKGNIY SLNEGYAKDFDPAINEYLQRKKFPPDGSAPYGARYVGSMVADIHRTLVYGGIFLYPANKKSPSGKLRLLY ECNPIAYVMEKAGGLATTGDKDILDIVPTEIHQKAPVVMGSSEDVQEFLEIYRKHKAK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019395 |
ORF Size | 1014 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019395.3 |
RefSeq Size | 1433 bp |
RefSeq ORF | 1017 bp |
Locus ID | 14121 |
UniProt ID | Q9QXD6 |
Cytogenetics | 13 B3 |
Gene Summary | Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201048 | Fbp1 (untagged) - Mouse fructose bisphosphatase 1 (Fbp1), (10ug) |
USD 457.00 |
|
MR205054 | Fbp1 (Myc-DDK-tagged) - Mouse fructose bisphosphatase 1 (Fbp1) |
USD 457.00 |
|
MR205054L3 | Lenti ORF clone of Fbp1 (Myc-DDK-tagged) - Mouse fructose bisphosphatase 1 (Fbp1) |
USD 757.00 |
|
MR205054L4 | Lenti ORF clone of Fbp1 (mGFP-tagged) - Mouse fructose bisphosphatase 1 (Fbp1) |
USD 757.00 |
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