Apof (NM_133997) Mouse Tagged ORF Clone

CAT#: MG204777

Apof (tGFP-tagged) - Mouse apolipoprotein F (Apof)

Product Images

Circular map for MG204777

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Apof
• Synonyms: AI255964
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204777 representing NM_133997
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGACTGTATGGGTGCTCAGCTCCTGACATGCATAGCCTCCGACTCATCCTGATGTCAATCCAGC
  TGCTTTGCTATCTCCTGCTGTGTCCTGTGGATGCCACTTCACACGGAGAGGCAACCAGTGTCTCTACGGT
  GCTCCCTCCCTCGAAGTTGGGATACCAGATGCAGACCTCAGACAAGCCCTTGTCCTGCCAGATGCTGCTC
  CCCAAGTCCCTGCCTGGCTTCACTTACATGCCCCCTGTCTCCAAATTCCTGGTAGGCCTGGCTCTAAGGA
  ATGCTCTGGAGGCAGCTGGCTGCCAGGCTGAGGTCTGGGCTCTTCAGCTTCAGCTTTACCGATTAGGAGG
  TGTGGAGGCTACACAAGCCCTCATCCACCATCTCCAGGAGCTCCAGAAGAGTGGACACACAGACCGGGAA
  GTGTCTGTGGATGCCCTGTCCTCTGCTCTGCAGCTCTTAGCCTGGGAGCAGCCAGGTCCAAAGAGGGCCA
  AACGTTCGATTTCCAACACAGACTGCGACAATGATCAGGAACAGAGTGTCCACAATGTGGTTGACCTACT
  ACCAGCAGTAGGCACCTACTACAACCTAGGCACAGCTTTGTATTACGCCATTAAGAACTGCTCTGACAAG
  GCCAAGGAACGAGGTCGAGATGGGGCCATAGATCTGGGTTATGATCTCTTGATGGCCATGGTAGGCGCGT
  CAGGGGGCCCAGCTGGTGCGGTGATCACGGCTGCTCTTAAGCCAGCGATGAAGGCTGGGGTTCAGCGGCT
  GATCCAGTATTATTATGATGAGAAAGAGGTGACCACCCCTCAGCCAGAGGTGACCACCCATCAGCCAGAG
  ACCGGAAAGGATGCCACCACAGATATCGGTGTTGTGGAAGAAATAGCCATGTCTAATTTTGTGTCAGAAG
  TTGAAAGTACAACTTCTAACTGGGAGTGGCCTTTATTAAAGAACTATGGTGTCTTGGCGTACAAGAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204777 representing NM_133997
  Red=Cloning site Green=Tags(s)
  
  MAGLYGCSAPDMHSLRLILMSIQLLCYLLLCPVDATSHGEATSVSTVLPPSKLGYQMQTSDKPLSCQMLL
  PKSLPGFTYMPPVSKFLVGLALRNALEAAGCQAEVWALQLQLYRLGGVEATQALIHHLQELQKSGHTDRE
  VSVDALSSALQLLAWEQPGPKRAKRSISNTDCDNDQEQSVHNVVDLLPAVGTYYNLGTALYYAIKNCSDK
  AKERGRDGAIDLGYDLLMAMVGASGGPAGAVITAALKPAMKAGVQRLIQYYYDEKEVTTPQPEVTTHQPE
  TGKDATTDIGVVEEIAMSNFVSEVESTTSNWEWPLLKNYGVLAYKR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133997
• ORF Size: 978 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133997.2, NP_598758.1
• RefSeq Size: 1930 bp
• RefSeq ORF: 981 bp
• Locus ID: 103161
• UniProt ID: Q91V80
• Cytogenetics: 10 76.5 cM
• Gene Summary: Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.[UniProtKB/Swiss-Prot Function]