Sult1b1 (NM_019878) Mouse Tagged ORF Clone

CAT#: MG204165

Sult1b1 (tGFP-tagged) - Mouse sulfotransferase family 1B, member 1 (Sult1b1)

Product Images

Circular map for MG204165

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sult1b1
• Synonyms: ST1B1; St2b2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204165 representing NM_019878
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGCCTCAGAAGACGTTTGGAGAAAAGATCTGAAGATGATCCATGGCTACCCCATGATCTATGCTT
  TTGCACTCAATTGGGAAAGGATTGAAGAGTTCCAGAGCACACCAGGTGACATTGTAATAACCACTTACCC
  TAAATCAGGTACTACTTGGCTTAGTGAGATTGTAGACATGGTTCTAAATGATGGAAATGTTGAAAAATGT
  AAGAGAGATGTTATCACCTCCAAAGTTCCAATGTTGGAACTGAGTGTTCCTGGAATAAGAATATCAGGTG
  TTGAACTCTTGAAGAAAACTCCATCACCTCGGATAATAAAGACACATCTTCCAATCGATCTACTCCCAAA
  ATCCTTCTGGGAGAACAAGTGCAAGATGATTTACCTTGCTCGAAATGGCAAGGATGTTGCTGTCTCCTAT
  TATCATTTTGATCTGATGAATAGTATTAATCCTCTTCCTGGCACCTGGGAAGAATATCTGGAGAAATTCC
  TAGCTGGAAATGTGGCCTATGGTTCATGGTTTGATCATGTTAAGAGTTGGTGGGAAAAGAGGGAAGAGCA
  TCCTTTACTTTACTTATACTATGAAGAATTGAAACAGAACCCAAAGAAAGAAATCAAGAAGATAGCCAGC
  TTTCTAGACAAGACCTTGGATGAAGAGGCCTTGGACAGGATCGTCCATCACACCTCCTTTGAAATGATGA
  AGGAAAACCCCCTGGTCAATTACACCCATCTGCCCACAGCAATGATGGACCACAGCAAGTCCCCTTTCAT
  GAGAAAAGGTATTGTTGGGGACTGGAAAAATTACTTCACAATGACCCAAACTGAGCAATTTGATGCTGTC
  TATAAGAAGAAGATGTCTGGAACAACACTTGAGTTCTGCACAGACATTCAGAGTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204165 representing NM_019878
  Red=Cloning site Green=Tags(s)
  
  MSASEDVWRKDLKMIHGYPMIYAFALNWERIEEFQSTPGDIVITTYPKSGTTWLSEIVDMVLNDGNVEKC
  KRDVITSKVPMLELSVPGIRISGVELLKKTPSPRIIKTHLPIDLLPKSFWENKCKMIYLARNGKDVAVSY
  YHFDLMNSINPLPGTWEEYLEKFLAGNVAYGSWFDHVKSWWEKREEHPLLYLYYEELKQNPKKEIKKIAS
  FLDKTLDEEALDRIVHHTSFEMMKENPLVNYTHLPTAMMDHSKSPFMRKGIVGDWKNYFTMTQTEQFDAV
  YKKKMSGTTLEFCTDIQSA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019878
• ORF Size: 897 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019878.4
• RefSeq Size: 2607 bp
• RefSeq ORF: 900 bp
• Locus ID: 56362
• UniProt ID: Q9QWG7
• Cytogenetics: 5 E1
• Gene Summary: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates L-DOPA and D-DOPA, tyrosine isomers such as DL-m-tyrosine, dopamine and thyroid hormones.[UniProtKB/Swiss-Prot Function]