Arrdc4 (NM_025549) Mouse Tagged ORF Clone

CAT#: MG204099

Arrdc4 (tGFP-tagged) - Mouse arrestin domain containing 4 (Arrdc4), transcript variant 2

Product Images

Circular map for MG204099

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arrdc4
• Synonyms: 2410003C09Rik; AV216361
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204099 representing NM_025549
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGGCGAGGCGGGAGCCGATGGTCCTCGGGGCCGTGTCAAGAGCTTGGGGCTAGTGTTCGAAGATG
  AGAGCAAGGGCTGCTACTCAAGCGGCGAGACAGTGGCCGGGCACGTGCTGCTGGAGGCGGCAGAGCCGGT
  GGCCCTGCGCGGACTGCGCCTGGAGGCCCAGGGCCGTGCCACCTCTGCCTGGGGCCCGAGCGCTGGGGCC
  AGGGTCTGCATCGGTGGGGGCTCTCCCGCAGCCTCCTCAGAAGTGGAATACTTGAACCTGCGGTTGAGTC
  TGCTGGAGGCCCCAGCTGGTGAAGGTGTCACCTTGTTACAACCAGGAAAACACGAGTTTCCCTTTCGCTT
  TCAGCTTCCGTCTGAACCTTTGGCAACATCGTTTACTGGGAAGTATGGCAGTATTCAGTACTGTGTGAGG
  GCTGTTTTGGAACGACCCCAAGTTCCAGATCAGAGCGTCAGACGAGAGCTCCAGGTTGTCAGTCACGTGG
  ATGTCAACACACCGCCCTTATTGACTCCTATGCTGAAGACGCAGGAGAAAATGGTTGGCTGTTGGCTTTT
  CACCTCTGGTCCTGTGTCACTGAGCGTCAAGATCGAGAGAAAGGGCTACTGTAACGGAGAAGCTATCCCT
  ATCTATGCAGAAATAGAAAATTGTTCATCTCGGCTGGTTGTTCCCAAGGCAGCCATATTCCAAACCCAGA
  CGTACTTGGCTAGTGGAAAGACAAAGACAGTCCGGCACATGGTTGCCAATGTTCGAGGAAACCACATTGG
  TTCTGGGAGTACGGACACCTGGAATGGGAAGATGCTGAAGATCCCACCTGTCACCCCATCCATCCTGGAT
  TGCTGCATCATCAGAGTGGACTACTCCTTAGCTGTAATCCAAGCTTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204099 representing NM_025549
  Red=Cloning site Green=Tags(s)
  
  MGGEAGADGPRGRVKSLGLVFEDESKGCYSSGETVAGHVLLEAAEPVALRGLRLEAQGRATSAWGPSAGA
  RVCIGGGSPAASSEVEYLNLRLSLLEAPAGEGVTLLQPGKHEFPFRFQLPSEPLATSFTGKYGSIQYCVR
  AVLERPQVPDQSVRRELQVVSHVDVNTPPLLTPMLKTQEKMVGCWLFTSGPVSLSVKIERKGYCNGEAIP
  IYAEIENCSSRLVVPKAAIFQTQTYLASGKTKTVRHMVANVRGNHIGSGSTDTWNGKMLKIPPVTPSILD
  CCIIRVDYSLAVIQAS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025549
• ORF Size: 888 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025549.3, NP_079825.2
• RefSeq Size: 1097 bp
• RefSeq ORF: 891 bp
• Locus ID: 66412
• UniProt ID: A0A0B4J1F4
• Cytogenetics: 7 C
• Gene Summary: Functions as an adapter recruiting ubiquitin-protein ligases to their specific substrates (PubMed:27462458). Plays a role in endocytosis of activated G protein-coupled receptors (GPCRs) (By similarity). Through an ubiquitination-dependent mechanism plays also a role in the incorporation of SLC11A2 into extracellular vesicles (PubMed:27462458). May play a role in glucose uptake (By similarity).[UniProtKB/Swiss-Prot Function]