Elovl3 (BC016468) Mouse Tagged ORF Clone

CAT#: MG203622

Elovl3 (tGFP-tagged) - Mouse elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 3 (cDNA clone MGC:18360

Product Images

Circular map for MG203622

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Elovl3
• Synonyms: Cig30; CIN-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203622 representing BC016468
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACATCCATGAATTTCTCACGCGGGTTAAAAATGGACCTGATGCAACCCTATGACTTCGAGACGT
  TTCAGGACTTAAGGCCCTTTTTGGAGGAGTACTGGGTAAGCTCATTTCTCATAGTGGTCGTCTATCTGTT
  GCTCATCGTTGTTGGCCAGACCTACATGAGAACGCGGAAGAGCTTCAGCTTGCAGAGGCCTCTCATCCTC
  TGGTCCTTCTTCCTGGCAATATTCAGTATCCTGGGTACTCTGAGGATGTGGAAGTTTATGGCAACAGTGA
  TGTTTACAGTGGGCCTCAAGCAAACCGTGTGCTTTGCCATCTACACGGATGACGCCGTAGTCAGATTCTG
  GTCCTTTCTCTTTCTTCTCAGCAAGGTTGTTGAACTGGGAGACACGGCCTTCATCATCCTGCGTAAGCGT
  CCACTCATCTTTGTCCACTGGTACCACCACAGCACAGTGCTACTGTTCACAAGCTTTGGATACAAGAACA
  AAGTGCCTTCGGGTGGCTGGTTCATGACCATGAACTTTGGCGTCCATTCTGTCATGTACACTTACTACAC
  TATGAAGGCTGCCAAACTGAAGCATCCTAATCTTCTCCCCATGGTCATCACCAGCCTGCAGATTCTGCAG
  ATGGTTCTGGGCACCATCTTTGGCATACTGAATTACATCTGGAGGCAGGAGAAAGGATGCCACACAACAA
  CGGAACACTTCTTCTGGTCTTTTATGCTATATGGGACCTATTTCATCCTATTCGCTCACTTCTTCCACCG
  AGCCTACCTCAGGCCCAAGGGCAAAGTTGCATCCAAGAGCCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203622 representing BC016468
  Red=Cloning site Green=Tags(s)
  
  MDTSMNFSRGLKMDLMQPYDFETFQDLRPFLEEYWVSSFLIVVVYLLLIVVGQTYMRTRKSFSLQRPLIL
  WSFFLAIFSILGTLRMWKFMATVMFTVGLKQTVCFAIYTDDAVVRFWSFLFLLSKVVELGDTAFIILRKR
  PLIFVHWYHHSTVLLFTSFGYKNKVPSGGWFMTMNFGVHSVMYTYYTMKAAKLKHPNLLPMVITSLQILQ
  MVLGTIFGILNYIWRQEKGCHTTTEHFFWSFMLYGTYFILFAHFFHRAYLRPKGKVASKSQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC016468
• ORF Size: 815 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: BC016468, AAH16468
• RefSeq Size: 1502 bp
• RefSeq ORF: 815 bp
• Locus ID: 12686
• Cytogenetics: 19 38.75 cM
• Gene Summary: Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that exhibits activity toward saturated and unsaturated acyl-CoA substrates with higher activity toward C18 acyl-CoAs, especially C18:0 acyl-CoAs. May participate in the production of saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. Participates in the formation of certain VLCFA and triglycerides in certain cells of the hair follicles and the sebaceous glands, required for skin barrier function. Critical enzyme for lipid accumulation and metabolic activity in brown adipocytes during the early phase of the tissue recruitment. Plays a role in lipid storage and in resistance to diet-induced obesity.[UniProtKB/Swiss-Prot Function]