Arpin (NM_027420) Mouse Tagged ORF Clone

CAT#: MG202650

2610034B18Rik (tGFP-tagged) - Mouse RIKEN cDNA 2610034B18 gene (2610034B18Rik)

Product Images

Circular map for MG202650

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arpin
• Synonyms: 2610034B18Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202650 representing NM_027420
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCGCATCTACCAGGATAGCGCCCTCCGCAACAAGGCCGTGCAGAGCGCGCGCCTGCCGGGAACCT
  GGGACCCTGCCACCCACCAAGGGGGAAATGGCATCTTGCTGGAGGGGGAGCTCGTGGATGTATCTCGGCA
  CAGCATCTTGGATGCCCATGGCAGGAAGGAGCGCTACTATGTGCTGTATATCCAGCCCAGCTGTATCCAC
  AGGCGTAAGTTTGACCCCAAGGGAAATGAAATTGAGCCCAACTTCAGTGCCACCAGGAAGGTGAACACAG
  GCTTCCTCATGTCATCTTACAAGGTGGAGGCCAAGGGCGACACAGACCGGCTCACCCTGGAGGCGCTGAA
  GAGCCTGGTAAACAAGCCCCAGCTGCTGGAATTGACAGAGAGCCTCACCCCAGACCAGGCGGTGGCATTC
  TGGATGCCTGAGTCAGAGATGGAGGTCATGGAACTCGAACTGGGGACTGGAGTGCGATTAAAAACTCGGG
  GTGATGGTCCCTTCATAGATTCCTTAGCCAAACTGGAGCTGGGGACAGTGACCAAGTGTAATTTTGCTGG
  TGATGGAAAGACGGGAGCTTCCTGGACAGACAATATCATGGCCCAGAAGTCTTCAGAGAGGAACACAGCA
  GAGATCCGAGAGCAAGGAGACGGGGCAGAGGACGAGGAATGGGATGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202650 representing NM_027420
  Red=Cloning site Green=Tags(s)
  
  MSRIYQDSALRNKAVQSARLPGTWDPATHQGGNGILLEGELVDVSRHSILDAHGRKERYYVLYIQPSCIH
  RRKFDPKGNEIEPNFSATRKVNTGFLMSSYKVEAKGDTDRLTLEALKSLVNKPQLLELTESLTPDQAVAF
  WMPESEMEVMELELGTGVRLKTRGDGPFIDSLAKLELGTVTKCNFAGDGKTGASWTDNIMAQKSSERNTA
  EIREQGDGAEDEEWDD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027420
• ORF Size: 678 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027420.4
• RefSeq Size: 2185 bp
• RefSeq ORF: 681 bp
• Locus ID: 70420
• UniProt ID: Q9D0A3
• Cytogenetics: 7 D2
• Gene Summary: Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competetive with nucleation promoting factors. Participates in an incoherent feedforward loop at the lamellipodium tip where it inhibits the ARP2/2 complex in response to Rac signaling and where Rac also stimulates actin polymerization through the WAVE complex. Involved in steering cell migration by controlling its directional persistence (By similarity).[UniProtKB/Swiss-Prot Function]