Cdca4 (BC012953) Mouse Tagged ORF Clone

CAT#: MG202082

Cdca4 (tGFP-tagged) - Mouse cell division cycle associated 4 (cDNA clone MGC:12023 IMAGE:3603210)

Product Images

Circular map for MG202082

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cdca4
• Synonyms: 2410018C03Rik; HEPP; SEI-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202082 representing BC012953
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTTGTCAAGCTCCAGCTCTGTCACATGCTAGTGGAGCCCAATCTCTGCCGCTCGGTCCTCATCG
  CCAACACAGTCCGGCAGATCCAGGAGGAAATGAGCCAGGATGGTGTGTGGCATGGGATGGCACCCCAGAA
  TGTAGATCGGGCACCAGTTGAGCGCCTGGTGTCCACAGAGATCCTGTGTCGTACAGTGAGGGGAGCTGAG
  GAAGAGCACCCTGCTCCTGAACTGGAAGATGCTCCCTTGCAAAACTCGGTTTCCGAGCTCCCCATCGTTG
  GCTCAGCACCAGGGCAAAGGAACCCTCAGAGCAGCCTCTGGGAGATGGACAGCCCACAAGAAAACAGGGG
  AAGCTTTCAGAAGTCACTGGACCAGATATTTGAGACCCTGGAGAACAAAAACTCCAGTTCAGTGGAGGAA
  CTCTTCTCAGATGTGGACAGCTCCTACTATGACCTGGACACAGTGCTAACAGGAATGATGACTGGGACCA
  AGTCCAGTCTCTGCAATGGCCTTGAGGGCTTTGCTGCAGCCGCCCCTCCTCCCAGTTCCACTTGCAAGTC
  TGACCTGGCTGAGCTGGACCATGTGGTAGAGATTCTGGTGGAGACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202082 representing BC012953
  Red=Cloning site Green=Tags(s)
  
  MSLVKLQLCHMLVEPNLCRSVLIANTVRQIQEEMSQDGVWHGMAPQNVDRAPVERLVSTEILCRTVRGAE
  EEHPAPELEDAPLQNSVSELPIVGSAPGQRNPQSSLWEMDSPQENRGSFQKSLDQIFETLENKNSSSVEE
  LFSDVDSSYYDLDTVLTGMMTGTKSSLCNGLEGFAAAAPPPSSTCKSDLAELDHVVEILVET
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC012953
• ORF Size: 608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: BC012953, AAH12953
• RefSeq Size: 1856 bp
• RefSeq ORF: 608 bp
• Locus ID: 71963
• Cytogenetics: 12 F1
• Gene Summary: May participate in the regulation of cell proliferation through the E2F/RB pathway (By similarity). May be involved in molecular regulation of hematopoietic stem cells and progenitor cell lineage commitment and differentiation.[UniProtKB/Swiss-Prot Function]