Nrsn1 (BC052019) Mouse Tagged ORF Clone

CAT#: MG201937

Nrsn1 (tGFP-tagged) - Mouse neurensin 1 (cDNA clone MGC:62360 IMAGE:5708003)

Product Images

Circular map for MG201937

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nrsn1
• Synonyms: Neuro-p24, Neurensin-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201937 representing BC052019
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCTTGCAGCAACACCTGTGGGTCCAGGCGAGCCCAGGCTGACACCGAGGGTGGGTACCAGCAGC
  GCTATGGAGTTCGGTCCTACCTGCATCAGTTTTATGAGGACTGCACTGCCTCTATCTGGGAGTATGAGGA
  TGATTTCCAGATCCAGAGATCGCCTAACAGGTGGAGCTCAGTATTCTGGAAGGTCGGACTCATCTCAGGC
  ACTGTCTTTGTGATTCTCGGACTAACTGTCCTGGCAGTGGGCTTTCTTGTGCCCCCCAAAATCGAAGCTT
  TTGGCGAAGCTGATTTCATGGTGGTGGACACACATGCTGTGAAGTACAACGGCGCCCTGGACACATGCAA
  GCTGGCGGGGGCTGTGCTCTTCTGCATCGGGGGTACATCCATGGCAGGGTGCCTACTGATGTCTGTGTTT
  GCCAAGAGCTATTCCAAAGAAGAAAAGTTCCTTCAACAGAAGTTTAAAGAGCGAATCGCAGACATTAAAG
  CCCATACCCAGCCCATCACTAAAGCTCCAGGCCCAGGGGACACCAAGATCCCTGTCACTTTGTCCAGAGT
  TCAGAATGTGCAGCCTCTATCAGCCACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201937 representing BC052019
  Red=Cloning site Green=Tags(s)
  
  MTSCSNTCGSRRAQADTEGGYQQRYGVRSYLHQFYEDCTASIWEYEDDFQIQRSPNRWSSVFWKVGLISG
  TVFVILGLTVLAVGFLVPPKIEAFGEADFMVVDTHAVKYNGALDTCKLAGAVLFCIGGTSMAGCLLMSVF
  AKSYSKEEKFLQQKFKERIADIKAHTQPITKAPGPGDTKIPVTLSRVQNVQPLSAT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052019
• ORF Size: 590 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: BC052019, AAH52019
• RefSeq Size: 2005 bp
• RefSeq ORF: 590 bp
• Locus ID: 22360
• Cytogenetics: 13 A3.1
• Gene Summary: May play an important role in neural organelle transport, and in transduction of nerve signals or in nerve growth (PubMed:12445626, PubMed:16527258, PubMed:16696124, PubMed:9191101). May play a role in neurite extension (PubMed:26430118).[UniProtKB/Swiss-Prot Function]