Timm22 (NM_019818) Mouse Tagged ORF Clone

CAT#: MG201879

Timm22 (tGFP-tagged) - Mouse translocase of inner mitochondrial membrane 22 homolog (yeast) (Timm22)

Product Images

Circular map for MG201879

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Timm22
• Synonyms: Tim22
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201879 representing NM_019818
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTACCGCCCCCAAGGCCGGGGGTTCAGCTCCTGAGGCAGCGGGTTCTGCCGAAGCTCCGTTGC
  AGTACAGCCTTCTTCTTCAGTACCTGGTGGGCGACAAGCGTCAGCCCCGGCTTCTGGAGCCTGGCAGCCT
  GGGCGGGATCCCGAGTCCTGCCAAGAGCGAGGAGCAGAAGATGATCGAGAGGGCAATGGAAAGTTGCGCC
  TTCAAGGCTGTGCTGGCCTGTGTAGGAGGGTTTGTCTTGGGAGGCGCGTTTGGTATCTTTACTGCTGGCA
  TTGATACCAACGTAGGCTTTGACCCAAAGGACCCTTACCGGACACCAACTGCAAAAGAAGTCCTGAAAGA
  CATGGGACAGAGAGGAATGTCCTATGCCAAAAACTTTGCCATTGTGGGCGCCATGTTTTCATGTACTGAG
  TGTCTGGTAGAGTCTTACCGGGGAAAGTCGGACTGGAAGAACAGCGTCATCAGTGGCTGCATCACTGGCG
  GAGCCATCGGCTTCCGAGCTGGAGTAAAGGCCGGGGCCATAGGTTGTGGAGGGTTTGCTGCTTTCTCTGC
  TGCAATCGATTATTACCTACGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201879 representing NM_019818
  Red=Cloning site Green=Tags(s)
  
  MAATAPKAGGSAPEAAGSAEAPLQYSLLLQYLVGDKRQPRLLEPGSLGGIPSPAKSEEQKMIERAMESCA
  FKAVLACVGGFVLGGAFGIFTAGIDTNVGFDPKDPYRTPTAKEVLKDMGQRGMSYAKNFAIVGAMFSCTE
  CLVESYRGKSDWKNSVISGCITGGAIGFRAGVKAGAIGCGGFAAFSAAIDYYLR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019818
• ORF Size: 582 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019818.5
• RefSeq Size: 2771 bp
• RefSeq ORF: 585 bp
• Locus ID: 56322
• UniProt ID: Q9CQ85
• Cytogenetics: 11 B5
• Gene Summary: Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps (By similarity).[UniProtKB/Swiss-Prot Function]