Mcee (NM_028626) Mouse Tagged ORF Clone

CAT#: MG201564

Mcee (tGFP-tagged) - Mouse methylmalonyl CoA epimerase (Mcee)

Product Images

Circular map for MG201564

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mcee
• Synonyms: 1110007A04Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201564 representing NM_028626
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGTGTAGTGAAGGCCGCCGCGTTGGCTGCGGGCGCTACAGGGCTTTTCTCCAGAGTCCAAACTT
  CAGTTGCAATAGGGAGAAGTTTTTCCACACCACAGTCCCAGTTTCAAGAATCCAGTCCTGTGTGGAAGCT
  GGGTCGGCTCAATCACGTGGCTGTAGCAGTGCCAGATTTGGAAAAGGCCTCGTCATTTTACAGGGATGTT
  CTAGGGGCCCAGGTGAGTGAGGTGGTCCCTCTGCCGGAACATGGAGTATCTGTGGTTTTTGTCAACCTGG
  GAAATACCAAGATGGAACTGCTTCATCCACTGGGGAGTGATAGTCCAATCACAGGCTTTCTGCAGAAGAA
  CAAGGCTGGAGGAATGCATCACGTCTGCATCGAGGTGGACAACATCAGTGCAGCTGTGATGGATCTGAAG
  AAGAAGAAGATCCGTAGTCTGAGTGATGAGGCCAAAATAGGAGCACATGGGAAACCTGTGATTTTCCTCC
  ATCCCAAAGACTGTGGTGGGGTCCTTGTAGAACTGGAGCAAGCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201564 representing NM_028626
  Red=Cloning site Green=Tags(s)
  
  MRRVVKAAALAAGATGLFSRVQTSVAIGRSFSTPQSQFQESSPVWKLGRLNHVAVAVPDLEKASSFYRDV
  LGAQVSEVVPLPEHGVSVVFVNLGNTKMELLHPLGSDSPITGFLQKNKAGGMHHVCIEVDNISAAVMDLK
  KKKIRSLSDEAKIGAHGKPVIFLHPKDCGGVLVELEQA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028626
• ORF Size: 534 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028626.2
• RefSeq Size: 714 bp
• RefSeq ORF: 537 bp
• Locus ID: 73724
• UniProt ID: Q9D1I5
• Cytogenetics: 7 C
• Gene Summary: Methylmalonyl-CoA epimerase involved in propionyl-CoA metabolism.[UniProtKB/Swiss-Prot Function]