Polr2g (NM_026329) Mouse Tagged ORF Clone
CAT#: MG201451
- TrueORF®
Polr2g (tGFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide G (Polr2g)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_026329" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Polr2g |
Synonyms | 2410046K11Rik; A230108L04Rik; C76415; RBP7; Rpo2-7l |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG201451 representing NM_026329
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTTATCACATTTCCCTGGAGCACGAGATCCTCCTGCACCCACGATACTTCGGTCCAAACTTGCTCA ACACGGTGAAGCAGAAGCTGTTTACCGAGGTGGAGGGGACCTGCACTGGGAAATATGGCTTTGTAATTGC TGTCACCACCATCGACAATATTGGTGCTGGTGTGATCCAGCCAGGCCGAGGTTTTGTTCTTTATCCAGTG AAATACAAAGCTATTGTTTTCCGGCCCTTTAAAGGTGAAGTGGTGGATGCTGTGGTCACTCAGGTCAACA AGGTTGGACTTTTCACAGAAATTGGGCCTATGTCTTGCTTCATCTCTCGACATTCCATCCCTTCAGAGAT GGAGTTTGATCCAAATTCAAACCCCCCTTGCTATAAGACCATGGACGAGGACATTGTGATTCAGCAGGAC GATGAGATACGCTTGAAGATTGTAGGCACGCGTGTAGACAAGAATGACATTTTTGCCATTGGCTCTCTGA TGGACGACTACTTGGGGCTGGTGAGC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201451 representing NM_026329
Red=Cloning site Green=Tags(s) MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPV KYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFDPNSNPPCYKTMDEDIVIQQD DEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_026329 |
ORF Size | 516 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_026329.1, NP_080605.1 |
RefSeq Size | 869 bp |
RefSeq ORF | 519 bp |
Locus ID | 67710 |
UniProt ID | P62488 |
Cytogenetics | 19 A |
Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. Binds RNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200405 | Polr2g (untagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide G (Polr2g), (10ug) |
USD 300.00 |
|
MR201451 | Polr2g (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide G (Polr2g) |
USD 300.00 |
|
MR201451L3 | Lenti ORF clone of Polr2g (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide G (Polr2g) |
USD 600.00 |
|
MR201451L4 | Lenti ORF clone of Polr2g (mGFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide G (Polr2g) |
USD 600.00 |
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