Alox5ap (NM_009663) Mouse Tagged ORF Clone

CAT#: MG201253

Alox5ap (tGFP-tagged) - Mouse arachidonate 5-lipoxygenase activating protein (Alox5ap)

Product Images

Circular map for MG201253

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Alox5ap
• Synonyms: Flap
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201253 representing NM_009663
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCAAGAGGCTGTGGGCAACGTTGTGCTCCTGGCCCTTGTCACCCTCATCAGCGTGGTCCAGAATG
  CGTTCTTTGCCCACAAGGTGGAGCATGAAAGCAAGGCGCATAATGGGAGAAGCTTCCAGAGGACCGGGAC
  TCTTGCCTTTGAGCGGGTCTACACTGCCAACCAGAACTGCGTAGATGCGTACCCCACTTTCCTTGTGGTA
  CTCTGGACTGCAGGACTACTTTGCAGCCAAGTCCCTGCCGCCTTCGCCGGACTGATGTACCTGTTTGTGA
  GGCAAAAATACTTTGTCGGCTATCTGGGAGAGAGAACTCAGAGCACCCCTGGCTACATCTTCGGCAAGCG
  GATCATCCTGTTTCTGTTCCTCATGTCCTTCGCCGGGATACTCAACCATTACCTCATCTTCTTCTTCGGA
  AGCGACTTTGAGAACTACATCAGAACGGTAAGCACGACGATCTCCCCGCTGCTTCTCATCCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201253 representing NM_009663
  Red=Cloning site Green=Tags(s)
  
  MDQEAVGNVVLLALVTLISVVQNAFFAHKVEHESKAHNGRSFQRTGTLAFERVYTANQNCVDAYPTFLVV
  LWTAGLLCSQVPAAFAGLMYLFVRQKYFVGYLGERTQSTPGYIFGKRIILFLFLMSFAGILNHYLIFFFG
  SDFENYIRTVSTTISPLLLIP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009663
• ORF Size: 483 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009663.1, NP_033793.1
• RefSeq Size: 872 bp
• RefSeq ORF: 486 bp
• Locus ID: 11690
• UniProt ID: P30355
• Cytogenetics: 5 G3
• Gene Summary: Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes (By similarity).[UniProtKB/Swiss-Prot Function]