Sf3b6 (NM_025323) Mouse Tagged ORF Clone
CAT#: MG200641
- TrueORF®
0610009D07Rik (tGFP-tagged) - Mouse RIKEN cDNA 0610009D07 gene (0610009D07Rik)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_025323" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Sf3b6 |
Synonyms | 0610009D07Rik; 6030419K15Rik; AV001342; Sf3b14 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG200641 representing NM_025323
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGATGCAAGCGGCTAAGAGGGCGAATATTCGCCTCCCACCTGAAGTAAATCGGATTTTGTATATAA GGAATTTGCCATACAAAATCACAGCAGAAGAAATGTATGATATATTTGGGAAATATGGACCTATTCGTCA AATCAGAGTAGGGAATACACCTGAAACCAGAGGAACAGCCTATGTGGTCTATGAGGACATCTTTGATGCT AAGAATGCCTGCGACCACCTATCAGGATTCAACGTTTGTAACAGATACCTTGTGGTTTTGTATTACAATG CTAACCGGGCATTTCAGAAGATGGACACCAAGAAGAAGGAGGAGCAGCTGAAACTTCTCAAGGAAAAGTA TGGCATCAACACAGATCCTCCCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200641 representing NM_025323
Red=Cloning site Green=Tags(s) MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDA KNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINTDPPK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025323 |
ORF Size | 375 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025323.2, NP_079599.1 |
RefSeq Size | 1133 bp |
RefSeq ORF | 378 bp |
Locus ID | 66055 |
UniProt ID | P59708 |
Cytogenetics | 12 A1.1 |
Gene Summary | Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203917 | 0610009D07Rik (untagged) - Mouse RIKEN cDNA 0610009D07 gene (0610009D07Rik), (10ug) |
USD 150.00 |
|
MR200641 | 0610009D07Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 0610009D07 gene (0610009D07Rik) |
USD 150.00 |
|
MR200641L3 | Lenti ORF clone of 0610009D07Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 0610009D07 gene (0610009D07Rik) |
USD 450.00 |
|
MR200641L4 | Lenti ORF clone of 0610009D07Rik (mGFP-tagged) - Mouse RIKEN cDNA 0610009D07 gene (0610009D07Rik) |
USD 450.00 |
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