Cklf (BC027684) Mouse Tagged ORF Clone

CAT#: MG200194

Cklf (tGFP-tagged) - Mouse chemokine-like factor (cDNA clone MGC:41679 IMAGE:1346032)

Product Images

Circular map for MG200194

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cklf
• Synonyms: Cklf2, Cklf6, C32, CKLF1, CKLF3, CKLF4, UCK-1, HSPC224, 1810018M11Rik, CKLF5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200194 representing BC027684
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACTCCACGGCCGGTCGTAAGCCGTCGGCCCTTCTGCTGTACTCTGAAATGCTTTGTGAAGTTCC
  TGCGGTTGGTGGTAACTGTGACATCTATGATCTTTTTCATCGTGGGACAAGCCCCTGAACCATATATCGT
  CATCACTGGGTTTGAAGTCACCGTTATTTTCTGTTTCCTAGTCTTATACACGTGTGGACTTGACAAGATA
  ATGAGATCTTTCTTTTGGCCTTTGCTTGTAAGTGATGAACTTCAACCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200194 representing BC027684
  Red=Cloning site Green=Tags(s)
  
  METPRPVVSRRPFCCTLKCFVKFLRLVVTVTSMIFFIVGQAPEPYIVITGFEVTVIFCFLVLYTCGLDKI
  MRSFFWPLLVSDELQP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC027684
• ORF Size: 260 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027684
• RefSeq Size: 1240 bp
• RefSeq ORF: 260 bp
• Locus ID: 75458
• Cytogenetics: 8 D3
• Gene Summary: May play an important role in inflammation and regeneration of skeletal muscle.[UniProtKB/Swiss-Prot Function]