Gak (NM_001282052) Mouse Untagged Clone
CAT#: MC229364
Gak (untagged) - Mouse cyclin G associated kinase (Gak), transcript variant 3
"NM_001282052" in other vectors (1)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Symbol | Gak |
Synonyms | D130045N16Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC229364 representing NM_001282052
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGAGGAAGAGATCACAAGGAACACCACACCCATGTACAGAACACCAGAAATTGTAGACCTGTATT CCAACTTCCCCATTGGCGAAAAGCAGGATATCTGGGCACTGGGCTGTATCTTATACCTGCTGTGTTTCCG GCAGCATCCTTTTGAGGATGGAGCAAAACTTCGGATAGTCAATGGGAAGTATTCCATTCCTGTGAATGAC ACTCGTTACACAGTCTTCCATGACCTTATTCGTGCCATGCTAAAGGTCAACCCAGAGGAGAGGCTGTCCA TTGCTGAAGTGGTCCGACAACTGCAGGAAATTGCAGCAGCTAGGAATGTGAACCCCAAAGCTCCCATCAC AGAGCTGCTGGAGCAGAATGGTGGCTATGGGAACTCAGGGCCTTCCCGAGCACAACCACCTTGCGGGGGC ACCGTGAACAGCAGTGGAGTTTTGGCTCTGGCAGAGTATGACCAGCCCTACGGTGGGTTTCTTGATATTC TGCGGGGTGGGACCGAACGGCTCTTCACCAACCTCAAGGATACTTCCTCCAAGGTCATCCAGTCTGTGGC TAACTATGCAAAGGGTGATCTGGACATATCTTACATCACATCCAGGATTGCAGTGATGTCATTCCCAGCA GAAGGTGTGGAGTCTGCAATCAAAAACAATATAGAGGATGTGCGAATGTTTCTGGATGCTAAGCATCCAG GACATTATGCTGTCTACAACCTTTCTCCAAGGATATACCGGGCTTCCAAGTTTCACAACCGGGTCACTGA GTGTGGCTGGGCAGTCAGACGGGCACCACATCTCCACAGTTTGTATACTCTATGTAGGAGCATGCATGCC TGGCTCCGGGAAGACCACAGGAACGTCTGTGTTGTGCATTGCATGGATGGGAGAGCTGCATCTGCTGTGG CAGTCTGTGCATTCCTGTGCTTCTGCCGTCTCTTCAGCACTGCAGAGGCTGCTGTGTACATGTTCAGCAT GAAGCGCTGCCCACCAGGCATTTGGCCATCCCACAAAAGGTACATTGAATATGTATGTGACATGGTGGCA GAGGAGCCCATCACGCCCCATAGCAAGCCAATGCTGGTGAAATCTGTTGTCATGACCCCCGTGCCACTGT TCAGCAAGCAGAGGAATGGCTGCCGACCGTTCTGTGAGGTCTACGTTGGAGAGGAGCGTGTCACCACCAC GTCGCAGGAATATGACAGAATGAAGGAATTTAAAATTGAGGATGGCAAGGCTGTCATCCCCCTGGGCGTA ACAGTTCAAGGAGACGTGCTCATCATCATCTACCATGCCAGGGCCACACTGGGAGGGAGGCTGCAGGCTA AGATGGCGTCCATGAAAATGTTCCAGATCCAGTTCCACACTGGGTTCGTGCCTCGAAACGCAACCACTGT GAAATTTGCAAAATATGACCTGGATGCTTGTGATATTCAAGAGAAGTACCCAGATCTGTTCCAGGTGAAC CTGGAAGTGGAGGTAGAGCCTAGAGACAGGCCCAGCCGAGAAGCTCCACCTTGGGAGAACACCAGCCTAA GGGGGTTAAACCCCAAGATCCTCTTTTCCAACAGGGAAGAGCAGCAGGACATTCTGTCTAAGTTTGGGAA GCCGGAGCTACCCCGGCAGCCGGGCTCCACAGCTCAGTATGATGCTGAGGCAGGGTCTCCGGAGGCTGAG ATCACAGAGTCGGACTCACCGCAGAGCAGCAGTACGGACACCAACCACTTTCTTCACACACTGGATTGGC AGGAGGAAAAAGAACCAGAGACTGGGTTAGACAATACCTCTCCTAAGGAGAGTCAGTCTGTCCTGATTGC AGACGGAGATGGAAGTGAAGTATCAGATGAAGAAGAGGCTTCATTCCCCAGTGAGGAGAGGAAACCAGGA GCTGGAGAAGATACACCAAGGCTGGCTGCTGGGACCAAACAGCAAGACTTAATATTTGATGTGGGCATGC TGGCTGCCCCACAGGAGCCTGTACAGCCCGAAGAAGGTGTTGATCTCCTGGGGCTACACTCTGAGGGGGA CTTAAGGCCTGCTGCCCCCTTACAGGCTTGTGGGGTCCCATCTAGCAACACTGACCTGTTGAGCTGCCTT CTTGAACCATCTGATGCTGCTCAAGTAGGGCCTCCTGGTGACCTGCTTGGTGGTGAGGCTCCACTGCTGC TAGCAAGCCCAGTTTCTCCTCTTGGGCTGCAGAACAACCTACAAGGAAAAGTCCCTGACACTGTGGACCC ATTTGACCAGTTCCTGCTGTCATCCAACTCAGACACCCAGCCCTGCTCCAAGCCTGATCTCTTTGGAGAG TTTCTCAACTCTGACTCTGTAGCTTCCTCAACTGCCTTCCCATCCACCCACAGTGCCCCACCCCCATCCT GTAGCACTGCCTTCCTGCACCTAGGGGATCTGCCAGCAGAGCCCAGCAAGGTAATAGCTTCATCCAGCCA CCCAGATCTGCTAGGAGGATGGGATACGTGGGCTGACACAGCTACACCTGGGCCAGCCTCCATTCCAGTA CCAGAAGGTACCCTCTTCTCTTCTGCAGGTCACCCAGCCCCTCCTGGCCCCAACCCCAGCCAAACCAAGT CTCAGAACCTTGACCCATTTGCTGATCTCAGTGATCTCAGCTCTAGCCTCCAAGGCCTGCCTGCTGGACT TCCTGCAGGGGGCTTTGTTGGCGCACCAGCCCCCACTCAAAAGAGCAACAGCCCCTGGCAGGCAAATCGT CCCACAGCCCCTGGAACCTCATGGACCCCCCAGGCCAAGCCAGCCCCCAGAGCCTCTGAACAGCTAAGGT CTCACTTCAGTGTGATCGGGGCCCGAGAAGAGAGGGGCGTCCGTGTGCCCAGCTTTGCCCAAAAGCCAAA GGTCTCAGAAAATGATTTTGAAGATCTTCTGCCTAATCAAGGCTTCTCTAAGTCTGACAAGAAGGGGCCA AAGACCATGGCAGAGATGCGGAAACAGGAACTTGCCAGAGATACAGACCCACTCAAATTGAAGCTTTTGG ACTGGATTGAAGGCAAGGAGAGGAACATTCGTGCACTGCTGTCCACTCTGCACACGGTATTGTGGGATGG GGAGAGCCGCTGGACACCTGTGAGTATGGCTGACCTGGTGACTCCAGAGCAGGTGAAGAAGCAGTACCGC CGTGCAGTGCTGGTAGTGCATCCTGATAAGGCCACAGGGCAGCCGTATGAACAGTATGCCAAGATGATCT TCATGGAGCTGAATGATGCATGGTCTGAGTTTGAAAACCAGGGCTCGAGGCCCCTCTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001282052 |
Insert Size | 3282 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001282052.1, NP_001268981.1 |
RefSeq Size | 4655 bp |
RefSeq ORF | 3282 bp |
Locus ID | 231580 |
UniProt ID | Q99KY4 |
Cytogenetics | 5 F |
Gene Summary | Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in its 5' UTR and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. |
Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR231575 | Gak (myc-DDK-tagged) - Mouse cyclin G associated kinase (Gak), transcript variant 3 |
USD 1,047.00 |
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