Zranb3 (NM_001285945) Mouse Untagged Clone

CAT#: MC229339

Zranb3 (untagged) - Mouse zinc finger, RAN-binding domain containing 3 (Zranb3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zranb3
• Synonyms: 4933425L19Rik; AH2; AI316834; C730006D09
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229339 representing NM_001285945
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTCACCATGCCTACAGCTGGTAGCAAGAAGAAGGCTCCCACACCGCAGATTTCTTGTTTAACAA
  GCGAATCATATACACAGCTGGATTTTTTACCGGACAAGCTTAGAACAAAACTGCTTCCGTTCCAAAAAGA
  TGGCATCGTTTTTGCCCTCAGAAGAGATGGCAGGTGTATGGTGGCTGATGAAATGGGTCTAGGAAAGACA
  ATCCAGGCGATTGCAATTGCTTACTTCTACAAAGAGGAGTGGCCCCTGCTGATAGTCGTCCCTTCATCTC
  TAAGATACCCTTGGATAGAAGAACTAGAAAAATGGATCCCAGAGCTAGAGCCAGAGGAAATCAATGTCGT
  CATGAACAAAACTGACATTGGGAGAATCCCGGGCAGCAGAGTGACAGTTCTGGGCTACGGTCTCTTAACC
  ACAGATGCAGAGACTTTGCTGGATGCACTGAACACGCAGAACTTCAGGGTCGTGATAGTGGATGAGTCAC
  ACTACATGAAGTCCAGAACTGCTGCCCGCAGCAAGATTCTCCTGCCCATGGTGCAGAAGGCCAGGAGAGC
  CATTCTTCTTACAGGAACCCCAGCTCTGGGAAGGCCTGAGGAGCTTTTCATGCAGATTGAGGCTCTTTTC
  CCACAAAAATTTGGAACTTGGATTGAGTATGCTAAAAGATACTGCAATGCACATGTCAGATACTTTGGCA
  AAAGGCGTCAGTGGGACTGTAGAGGGGCATCAAACCTTAGTGAGCTTCACCAGCTCCTAAATGACATAAT
  GATCAGAAGACTAAAGAGTGAAGTTTTAAGCCAGCTGCCCCCGAAAGTCAGACAGCGCATTCCCTTTGAC
  CTTCCCCCAGCAGCTGTCAAGGAACTGAATGCCAGCTTTGAAGAATGGCAGAAACTAATGAGAGCTCCAA
  ATTCAGGTGCCATGGAGACGGTTATGGGGTTGATAACACGCATGTTCAAACAGACTGCAATTGCCAAGGC
  AGGTGCAGTGAAGGACTATATTAAGATGCTGCTTCAGAACGACTCCCTTAAATTTCTGGTCTTTGCGCAC
  CATTTAAGTATGCTCCAGGCTTGCACAGAAGCAGTCATCGAAAGCAAGTCTCGTTACATCAGGATAGATG
  GAAGTGTTCCATCTTCAGAAAGAATTCATCTGGTTAATCAATTTCAGAAGGACCCCGATACTCGTGTGGC
  TATCCTGAGCATTCAGGCTGCTGGCCAGGGTTTAACGTTTACTGCTGCGAGTCACGTTGTCTTTGCTGAG
  TTGTACTGGGACCCTGGACATATCAAACAAGCAGAAGACCGAGCTCACCGAATCGGACAGTGCAGTTCTG
  TGAATATTCACTACCTTATTGCAAATGGTACTCTGGACAGCCTAATGTGGGCAATGCTGAATCGAAAGGC
  TCAGGTCACAGGGAGCACACTGAATGGCAGGAAGGAGAAACTCCAGGCTACGGAAGATGACAAGGAGAAG
  TGGGGTTTTCTGCAGTTTGCTGAAGCATGGACCCCAAGTGACAGTTTTGAAGAGCTCAAGGATTCTGTGT
  TTACTCACTTTGAAAAAGAGAAACAGCATGATATTCGATCATTCTTCTTACCAAAACTGAAAAAGAGACA
  GTTAGAAACGACCTGTGATGACCCAGAGGCATTCAAGGAGAAGATTACAGTGGCCTCGGACCCTAGAAAA
  ATGGCTACAAGTGATAGCACTGCTGACAAAAATGGGTGTGAGCCTGAAGCTAAAAGGTTGAAGTCCCTGA
  GCACCGAGGACCACAGCAGTGCCCTGGAGGAAGGACCGTCCTTGCAAGCCAGAGCCACATCCATGGAAGT
  TGTCCATGAGGTCAAGCCCCCATTGGCTTCTCCAGCCCTTCCTGAGAAGGGATGGCAGTGTGGCTTCTGC
  ACATTCCTCAACAACCCAGGCCTTCCATACTGTGAGATGTGTGAGAACCCTCGGAGCAGAGCTGCTGGCC
  GCAACCATCTTCAGGATAACAACAAAAATGATGAAGACGCTGCTCAGGAAAGCACCTCCAAAAGTGATCA
  AGCTGGTTTGGAGTGTGAAAGGCAATGCCCTGAACGGTTGGAAGCTGAGCAGTCGGCTAATAGCAAGGAA
  GAGGCATTGGAAGGTGGAGGGGAAGACAGACTGCCATCTCAGCCAGAGATTGGGCAGTTGAATAATTCAG
  GCACTCTGCCAGTGCGTGAGACCTTCATGTTTTGTGCAAGCAGAAACACCGACCGGATCCACCTCTATAC
  CAAGGATGGAAAGCCAATGAACTGCAATTTTATTCCTTTGGATATAAAGTTAGACCTTTGGGAAGACTTA
  CCAGCAACCTTTCAACTGAAACAGAATCGCTCTCTGATTCTAAGGTTCGTCCGAGAGTGGAGTAGTCTGA
  CTGCCATGAAGCAGAGAGTTCTCAGGAAAAGTGGTCAGCTGTTCTGCAGCCCACTGCTGGCTTCGGAAGA
  GATCACAAAGCAACAAGCCAAGGAAAATAATACTAGAAGATACATCACTAAAGAAGATGTTGCCAAGGCT
  TCCATGAATAAGGTGAAAAGTGATGGGGGCCACATCCGTCTCATCACCAAGGAATCCATGACCCAGGACT
  CTTCTCTAAAAAAGATTGATAGCGCCTGTGTCCCATCTCTGAATCCCTGCCCAGCGGATCTCACTGTTGA
  GCCATCCCCATCCAAAGGCTACATTCAGGCGGTGGATAAGGAAGGAAGGCCACTCTGCCTCCGCTGCCAG
  CATCCCACCTGCCAGCCTGAGCAAACAGCCAAAGCCAGTGCCTGGGACTCACGGTTCTGCTCCCTGAAAT
  GTCAGGAAGAATTCTGGATCCGATCCAATAACAGTTACCTGAGAGCCCAGGTGTTTGCAACAGAACATGG
  TGTGTGTCAGCACTGTGGTGTGGACGCACAGGAGCTCTTTCTGCGGATGAGAGATGCCCCTAAAAGTCAC
  AGGAAGAGTCTCCTCAATGCTGCCTGGACTGCAAAACTCCCACTGGAGCAGCTAAATGAGATGCTGAGAA
  ACCCAGGAGAAGGCCACTTCTGGCAGGTGGATCACATCAGGCCAGTGTATGAGGGAGGTGGACAGTGCTC
  CCTAGACAACCTGCAGACCCTCTGCACAGTGTGCCACAAGGAGAGAACTGCCCAGCAAGCCAAGGAAAGA
  AGTCAGGTGAGACGACTCTCTCTGGCAACAAAGCACGGATCAGACATCACCCGGTTTCTGGTGAAGAAGT
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285945
• Insert Size: 3222 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285945.1, NP_001272874.1
• RefSeq Size: 4085 bp
• RefSeq ORF: 3222 bp
• Locus ID: 226409
• UniProt ID: Q6NZP1
• Cytogenetics: 1 E3
• Gene Summary: DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks (By similarity).[UniProtKB/Swiss-Prot Function]