Mfn2 (NM_001285920) Mouse Untagged Clone
CAT#: MC228871
Mfn2 (untagged) - Mouse mitofusin 2 (Mfn2), transcript variant 1
"NM_001285920" in other vectors (1)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Symbol | Mfn2 |
Synonyms | D630023P19Rik; Fzo |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC228871 representing NM_001285920
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTGCTCTTTTCTCGATGCAACTCCATCGTCACCGTCAAGAAGGATAAGCGACACATGGCTGAAG TGAATGCTTCCCCTCTCAAGCACTTTGTCACTGCCAAGAAAAAGATCAATGGAATCTTTGAGCAGCTGGG GGCCTACATCCAAGAGAGCGCCAGCTTCCTTGAAGACACCCACAGGAACACAGAACTGGACCCGGTTACC ACGGAAGAGCAGGTCCTGGACGTCAAAGGGTACCTGTCCAAGGTCAGGGGTATCAGCGAAGTGCTGGCCA GGCGGCACATGAAGGTGGCTTTTTTTGGCCGGACGAGCAATGGGAAGAGCACCGTGATCAATGCCATGCT CTGGGACAAAGTTCTGCCATCTGGGATTGGTCATACCACCAATTGCTTCCTGCGGGTTGGGGGCACAGAT GGCCATGAGGCCTTCCTCCTCACAGAGGGCTCAGAAGAGAAGAAGAGTGTCAAGACTGTGAACCAACTGG CCCATGCCCTCCATCAGGACGAGCAGTTGCATGCAGGCAGCATGGTGAGTGTGATGTGGCCCAACTCCAA GTGTCCGCTCCTGAAGGATGACCTCGTGCTGATGGACAGCCCTGGGATCGATGTTACCACGGAGCTGGAC AGCTGGATTGATAAGTTTTGCCTGGATGCTGATGTGTTTGTGCTGGTGGCCAACTCAGAGTCCACGCTGA TGCAGACGGAGAAGCAGTTCTTCCACAAAGTGAGTGAACGTCTCTCCCGGCCCAACATCTTCATCCTGAA CAACCGCTGGGATGCGTCTGCCTCGGAGCCTGAGTACATGGAGGAGGTGCGGCGGCAGCACATGGAGCGC TGCACCAGCTTTCTGGTGGATGAGCTGGGCGTGGTGGATCGAGCTCAGGCTGGGGACCGGATCTTCTTCG TGTCTGCCAAGGAGGTTCTCAGCGCCAGGGTCCAGAAAGCCCAGGGCATGCCAGAAGGAGGCGGCGCTCT CGCAGAAGGTTTTCAAGTGAGGATGTTTGAGTTTCAGAATTTCGAGAGGCAGTTTGAGGAGTGCATTTCC CAGTCTGCAGTAAAGACCAAATTTGAGCAGCACACAGTCCGGGCCAAGCAGATTGCAGAGGCCGTTCGTC TCATCATGGATTCCCTGCACATCGCAGCTCAGGAGCAGCGGGTTTATTGCCTAGAAATGCGGGAAGAGCG GCAAGACCGGCTGAGGTTTATTGACAAGCAGCTGGAGCTCCTGGCTCAAGACTACAAGCTGCGAATTAAG CAGATTACGGAGGAAGTGGAAAGGCAGGTGTCCACAGCCATGGCTGAAGAGATCAGGCGCCTCTCTGTGC TAGTTGACGAGTACCAGATGGACTTCCACCCATCCCCAGTTGTCCTCAAGGTTTATAAGAACGAGCTGCA CCGCCATATAGAGGAAGGTCTGGGCCGGAACCTGTCTGACCGCTGCTCCACTGCCATTGCCAGTTCACTG CAGACTATGCAGCAGGACATGATAGACGGCTTGAAGCCCCTTCTTCCTGTATCTATGCGGAATCAGATAG ACATGCTGGTCCCTCGACAGTGTTTCTCCCTCAGCTATGACCTGAATTGTGACAAGCTGTGTGCTGACTT TCAGGAGGACATCGAGTTCCACTTCTCCCTTGGATGGACTATGCTAGTGAACAGGTTCCTGGGCCCCAAG AATAGCCGCCGGGCCTTGCTAGGCTACAGTGATCAGGTTCAGCGTCCTCTCCCTCTGACACCTGCCAACC CCAGCATGCCCCCCTTGCCACAGAGCTCCCTCACCCAGGAGGAGCTCATGGTCTCCATGGTTACTGGCCT GGCCTCTCTGACGTCTAGGACCTCCATGGGCATTCTTGTGGTCGGAGGAGTGGTGTGGAAGGCAGTGGGC TGGAGACTCATCGCCCTCTCCTTTGGACTGTATGGCCTCCTGTACGTCTATGAGCGACTGACCTGGACCA CCAAAGCCAAAGAGAGGGCCTTCAAGCGCCAGTTTGTGGAATACGCCAGTGAGAAGCTACAGCTCATCAT CAGTTACACCGGCTCTAACTGCAGCCACCAAGTCCAGCAGGAATTGTCTGGGACATTTGCTCATCTGTGC CAGCAAGTTGACATCACCCGAGATAATCTGGAGCAGGAAATTGCTGCCATGAACAAGAAAGTCGAGGCTC TGGATTCACTTCAGAGCAGAGCCAAACTGCTCAGGAATAAAGCTGGCTGGTTGGACAGCGAACTCAACAT GTTCACACACCAGTACCTGCAGCCCAGCAGATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001285920 |
Insert Size | 2274 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001285920.1, NP_001272849.1 |
RefSeq Size | 4559 bp |
RefSeq ORF | 2274 bp |
Locus ID | 170731 |
UniProt ID | Q80U63 |
Cytogenetics | 4 E1 |
Gene Summary | Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12527753, PubMed:23921378, PubMed:23620051). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (By similarity). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Plays an important role in the regulation of vascular smooth muscle cell proliferation (By similarity). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria (PubMed:23620051). Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (PubMed:23921556). Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions (PubMed:23921556).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript. All variants (1 through 7) encode the same protein. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR231082 | Mfn2 (myc-DDK-tagged) - Mouse mitofusin 2 (Mfn2), transcript variant 1 |
USD 1,039.00 |
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