Tle2 (NM_001252401) Mouse Untagged Clone

CAT#: MC228807

Tle2 (untagged) - Mouse transducin-like enhancer of split 2, homolog of Drosophila E(spl) (Tle2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tle2
• Synonyms: Grg2; mKIAA4188
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228807 representing NM_001252401
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCCTCAGGGAAGGCACCCGACCCCGCTGCAGTCTGGCCAGCCTTTCAAGTTCTCAGTACTGGAAA
  TCTGTGACCGGATCAAAGAGGAATTCCAGTTTCTTCAAGCTCAGTACCACAGCCTCAAGCTAGAATGTGA
  GAAGCTGGCCAGCGAGAAGACAGAAATGCAAAGGCATTATGTGATGTACTACGAGATGTCCTACGGACTC
  AACATTGAGATGCATAAACAGGCTGAGATTGTGAAACGCCTCAGTGCGATCTGTGCCCAGATGGTCCCGT
  TCCTCACTCAGGAGCATCAGCAGCAGGTGCTCCAGGCTGTGGACCGAGCCAAGCAGCAGCAGAATCAGCT
  CCAGCCGCTGTCCCACGCACCCCCCGTGCCTCTCACCCCGCGCCCAGCCGGCCTGGTGGGTGCCGGGGCC
  ACTGGGCTGCTGGCCCTATCTGGGGCACTGGCTGCGCAGGCCCAGCTGGTGGCTGCCGTAAAGGAAGACC
  GTGTGGGTGTGGACGCCGAGGGGTCCAGAGTGGACAGAGCTGCCAGCAGGAGTTCTTCCCCGTCTCCCCC
  TGAGAGTCTGGTGGAAGAGGACCATCCCAGCAGCCGAGGCGGCAGTGGGAAACAGCAGAGAGCTGAAGAC
  AAGGATCTGTCAGGGCCTTATGACAGTGAGGAAGACAAGAGTGACTATAACCTGGTAGTGGATGAGGACC
  AACCGTCAGAGCCCCCCAGCCCTGTGACCACCCCTTGTGGGAAGGCGCCCCTCTGCATTCCTGCCCGCAG
  GGACCTCACAGACAGTCCAGCCTCCTTGGCCTCCAGTTTGGGCTCACCACTCCCCAGAAGCAAAGACATA
  GCCCTGAACGATCTTCCCACAGGCACTCCTGCCTCCAGGTCATGTGGTACCTCTCCACCCCAGGACTCGT
  CCACCCCGGGGCCCAGCTCAGCCAGTCACCTCTGCCAGCTGGCGGCTCAGCCGGCAGCACCCACAGACAG
  CATCGCCCTGAGGAGTCCCCTGACCTTGTCCAGCCCTTTCACCTCATCCTTCAGCCTGGGCTCCCACAGC
  ACCCTCAATGGGGACCTCTCCATGCCTGGCTCCTATGTCGGCCTCCACCTGTCCCCCCAGGTCAGCAGCT
  CTGTCGTGTATGGACGCTCACCTCTGATGGCATTTGAATCGCACCCCCATCTCCGAGGCTCGTCTGTCTC
  CTTGCCTGGCATCCCTGTGGCTAAGCCGGCTTACTCCTTCCACGTGTCTGCGGATGGCCAGATGCAGCCT
  GTGCCCTTCCCGTCTGATGCTCTGGTAGGCACAGGCATCCCTCGCCACGCAAGGCAGCTACACACGCTGG
  CCCACGGTGAGGTGGTGTGTGCCGTCACCATCAGCAGCTCCACACAGCACGTGTACACAGGCGGCAAAGG
  ATGCGTGAAGGTGTGGGACGTGGGCCAGCCGGGTAGCAAGACCCCTGTGGCACAGCTGGATTGCCTGAAC
  CGAGACAACTACATCCGCTCCTGCAAGCTGCTGCCCGACGGGCAGAGCCTGATTGTAGGTGGCGAGGCCA
  GTACCCTGTCCATTTGGGACCTGGCAGCACCCACACCACGGATCAAAGCAGAGCTGACTTCGTCTGCCCC
  GGCCTGCTATGCTCTGGCCGTCAGTCCGGACGCCAAGGTCTGCTTCTCCTGCTGCAGCGACGGTAACATC
  GTGGTCTGGGACCTGCAGAACCAGGCCATGGTCAGACAGTTCCAGGGCCACACGGACGGGGCCAGCTGCA
  TCGACATATCAGACTACGGGACCCGGCTGTGGACTGGGGGCCTGGACAACACTGTGCGCTGCTGGGACCT
  GCGCGAGGGCCGCCAGCTTCAGCAGCATGACTTCAGTTCCCAGATTTTCTCATTGGGCCACTGTCCCAAT
  CAGGACTGGTTGGCTGTGGGGATGGAGAGCAGCCACGTGGAGGTCCTGCATGTGCGCAAGCCTGAGAAGT
  ACCAGCTCCGCCTCCATGAGAGCTGCGTGCTGTCACTCAAGTTCGCTTCCTGTGGACGCTGGTTTGTGAG
  CACAGGCAAGGACAACCTGCTCAATGCCTGGAGGACACCCTATGGGGCCAGCATTTTTCAGTCCAAAGAA
  TCATCTTCTGTACTGAGCTGCGACATCTCCAGGAATAATAAGTACATCGTGACAGGCTCAGGGGACAAGA
  AGGCCACTGTGTATGAGGTGGTGTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252401
• Insert Size: 2199 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001252401.1, NP_001239330.1
• RefSeq Size: 2721 bp
• RefSeq ORF: 2199 bp
• Locus ID: 21886
• Cytogenetics: 10 C1
• Gene Summary: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses different splice sites in two locations in the coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.