Rnf6 (NM_001256086) Mouse Untagged Clone

CAT#: MC228646

Rnf6 (untagged) - Mouse ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf6
• Synonyms: 1200013I08Rik; 5730419H05Rik; AA537053
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228646 representing NM_001256086
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGAATTAAAGGAAACTAGAAGTCTCAGGATGGATCCGTCTAGATCTAGATCAGGTGGGAGTGGAG
  AAGAGAGCTCATTTCAAGAAAATGAGAGAAGATGGCAGCAAGAACGTCTCCACAGAGAAGAAGCCTATTA
  TCAGTTTATTAATGAGCTCAGTGATGAAGATTATCGGCTAATGAGAGACCATAATCTTTTAGGCACCCCT
  GGAGAAATAACATCAGAGGAGCTGCAGCAGCGCTTAGAGAGAGCAAAGGAACAGCTGGCATCTCAGCCTG
  GCTCTGACAGCGCGGCAAGTGATGGAGACTCAGAAAGCCTTAGAGCACATTCAGATGAGGACTCTCTGCT
  GCGATGGCTGAATACCTTTCGGCGCACTGGGAATGTGACTCGAAGCGGACAGAATGGGAACCAAAGCTGG
  AGGGCCGTGAGTCGAACAAACCCGAACAGTGGCGAGTTCGGGTTTAGCCTGGAAATCCACATAAACCCTG
  ACAACAGAGGTTCTGAAATGCATGGAGAGGACTCGACGGACATCCCTCTGTCAGGTGTTAACAGAGAGCA
  TAGGCAGCAAAGACCATCGAGTCCTGTGGCTAGGCGAACCAGAAGCCAGACCTCCATGAGCTCCAGTGGT
  CCTAGGGGCAGGCGTGGTGCGAGGAGGCAGGGTTCTGTGCAAGGGTCATTTGCAACTCTGGGAAGGTTAA
  GGAATGGAATTGGAGTAGCCCTTGGTGTTCCCAGAGTTAGTGCCCCTCGCACTAATGTTATTAATTCCCA
  CACCAACCAGTCAGATGGTAGTACTCTCAGGCAAGGTGGACGGCAGCGGTTTGGAGCAGCACATATTTGG
  GAAAACGGGGCTAGAAGTAATGTTACAGTGAGGAACACAAACCAAAGATTAGAGCCAATAAGATTACGTC
  CTGCTTTCAGTAGTCGAAGCCGGTCCCCCATTCAGAGACAGAATGGTACTGTTCATCATAATTCCCAAAG
  GCAGGGTAGACCAGTACAGCAAACGGGTAGAAACAGGTCTGTTAGGCACAGAGGTGTAACGCGGGTCTTT
  TTAGAACAAGGTAGAGAACACAGAGGCACTGATTACACCCCGCTGTCCAACTCAAGACTTGTGTCAAGAA
  TAACAGTAGAAGAGGGAGAGTCTAGCAGGTCCTCAGCTGCCACCCAGCGTCATCCAGCAATCACACTGGA
  CCTGCAAGTCAGGAGGATTCGGCCTGGAGAAACTAGAGATAGGGATAGCATTGCAAATAGAACCCGATCT
  AGGGCGGGGCTGGCAGAAAGTACGGTTGAGAGCACCAGTGGGGGCTTCCACAGAACTATCTCTCACTTAG
  AGCGCTCAGGTGTCCGAACCTACGTGAGCACCATCACAGTTCCTCTTCGCAGGATTTCTGAGAATGACGT
  GGTCGAGCCATCCTCCGTGGCTCTTCGGTCAATTTTAAGGCAGATCATGACTGGGTTTGGAGAGCTGAGT
  TCTCTAATGGAGGTGGAGCCTGCGTCAGAGAATCAAAGCAATGGCCAACGTTTACCAGAGGTGTACCTAG
  AACTGAGTAATGGAGATGCAGCCGATGACAGTGGTCAGCATGGAAGAGCGTCCTCACAGGCCAGTCAGGC
  CCAGGACGGTGCTGAAATGCTAGCTGTGAGGGAGCCCGCCCCGCCCCAGGCTCGCCCCAGTGGCAGTAGA
  AGTAGAAGGCAGTTAGGAAGGGCGGACAGCGTAGTTGAGGCTGGAACATTACCCATTCTGCGCCTTGCTC
  ACTTCTTCTTACTGAATGAAGGCGATGATGATCCAATTCGTGGTTTAACCAAAGAGCAGATTGACAATCT
  TTCTACCAGGAGTTATGAACAAGACGGGGTTGACAGTGAGTTAGGTAAGGTGTGTAGCGTGTGCATCAGT
  GACTACGTGGCTGGGAACAAGCTCCGGCAGCTGCCTTGTCTGCACGAGTTTCACATTCACTGTATTGATC
  GCTGGCTGTCGGAGAACTGCACCTGCCCCGTCTGTCGGCGGCCTGTCTTAGAATTCGGAGCCACAAGCAG
  TGGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256086
• Insert Size: 2037 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256086.1, NP_001243015.1
• RefSeq Size: 3225 bp
• RefSeq ORF: 2037 bp
• Locus ID: 74132
• Cytogenetics: 5 G3
• Gene Summary: E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR and coding region which includes an upstream in-frame AUG start codon, compared to variant 1. The resulting isoform (2) is longer at the N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.