Rnf6 (NM_001256087) Mouse Untagged Clone

CAT#: MC228616

Rnf6 (untagged) - Mouse ring finger protein (C3H2C3 type) 6 (Rnf6), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf6
• Synonyms: 1200013I08Rik; 5730419H05Rik; AA537053
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228616 representing NM_001256087
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCGTCTAGATCTAGATCAGGTGGGAGTGGAGAAGAGAGCTCATTTCAAGAAAATGAGAGAAGAT
  GGCAGCAAGAACGTCTCCACAGAGAAGAAGCCTATTATCAGTTTATTAATGAGCTCAGTGATGAAGATTA
  TCGGCTAATGAGAGACCATAATCTTTTAGGCACCCCTGGAGAAATAACATCAGAGGAGCTGCAGCAGCGC
  TTAGAGAGAGCAAAGGAACAGCTGGCATCTCAGCCTGGCTCTGACAGCGCGGCAAGTGATGGAGACTCAG
  AAAGCCTTAGAGCACATTCAGATGAGGACTCTCTGCTGCGATGGCTGAATACCTTTCGGCGCACTGGGAA
  TGTGACTCGAAGCGGACAGAATGGGAACCAAAGCTGGAGGGCCGTGAGTCGAACAAACCCGAACAGTGGC
  GAGTTCGGGTTTAGCCTGGAAATCCACATAAACCCTGACAACAGAGGTTCTGAAATGCATGGAGAGGACT
  CGACGGACATCCCTCTGTCAGGTGTTAACAGAGAGCATAGGCAGCAAAGACCATCGAGTCCTGTGGCTAG
  GCGAACCAGAAGCCAGACCTCCATGAGCTCCAGTGGTCCTAGGGGCAGGCGTGGTGCGAGGAGGCAGGGT
  TCTGTGCAAGGGTCATTTGCAACTCTGGGAAGGTTAAGGAATGGAATTGGAGTAGCCCTTGGTGTTCCCA
  GAGTTAGTGCCCCTCGCACTAATGTTATTAATTCCCACACCAACCAGTCAGATGGTAGTACTCTCAGGCA
  AGGTGGACGGCAGCGGTTTGGAGCAGCACATATTTGGGAAAACGGGGCTAGAAGTAATGTTACAGTGAGG
  AACACAAACCAAAGATTAGAGCCAATAAGATTACGTCCTGCTTTCAGTAGTCGAAGCCGGTCCCCCATTC
  AGAGACAGAATGGTACTGTTCATCATAATTCCCAAAGGCAGGGTAGACCAGTACAGCAAACGGGTAGAAA
  CAGGTCTGTTAGGCACAGAGGTGTAACGCGGGTCTTTTTAGAACAAGGTAGAGAACACAGAGGCACTGAT
  TACACCCCGCTGTCCAACTCAAGACTTGTGTCAAGAATAACAGTAGAAGAGGGAGAGTCTAGCAGGTCCT
  CAGCTGCCACCCAGCGTCATCCAGCAATCACACTGGACCTGCAAGTCAGGAGGATTCGGCCTGGAGAAAC
  TAGAGATAGGGATAGCATTGCAAATAGAACCCGATCTAGGGCGGGGCTGGCAGAAAGTACGGTTGAGAGC
  ACCAGTGGGGGCTTCCACAGAACTATCTCTCACTTAGAGCGCTCAGGTGTCCGAACCTACGTGAGCACCA
  TCACAGTTCCTCTTCGCAGGATTTCTGAGAATGACGTGGTCGAGCCATCCTCCGTGGCTCTTCGGTCAAT
  TTTAAGGCAGATCATGACTGGGTTTGGAGAGCTGAGTTCTCTAATGGAGGTGGAGCCTGCGTCAGAGAAT
  CAAAGCAATGGCCAACGTTTACCAGAGGTGTACCTAGAACTGAGTAATGGAGATGCAGCCGATGACAGTG
  GTCAGCATGGAAGAGCGTCCTCACAGGCCAGTCAGGCCCAGGACGGTGCTGAAATGCTAGCTGTGAGGGA
  GCCCGCCCCGCCCCAGGCTCGCCCCAGTGGCAGTAGAAGTAGAAGGCAGTTAGGAAGGGCGGACAGCGTA
  GTTGAGGCTGGAACATTACCCATTCTGCGCCTTGCTCACTTCTTCTTACTGAATGAAGGCGATGATGATC
  CAATTCGTGGTTTAACCAAAGAGCAGATTGACAATCTTTCTACCAGGAGTTATGAACAAGACGGGGTTGA
  CAGTGAGTTAGGTAAGGTGTGTAGCGTGTGCATCAGTGACTACGTGGCTGGGAACAAGCTCCGGCAGCTG
  CCTTGTCTGCACGAGTTTCACATTCACTGTATTGATCGCTGGCTGTCGGAGAACTGCACCTGCCCCGTCT
  GTCGGCGGCCTGTCTTAGAATTCGGAGCCACAAGCAGTGGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256087
• Insert Size: 2004 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256087.1, NP_001243016.1
• RefSeq Size: 3286 bp
• RefSeq ORF: 2004 bp
• Locus ID: 74132
• UniProt ID: Q9DBU5
• Cytogenetics: 5 G3
• Gene Summary: E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same isoform (1).