Tcf12 (NM_001253864) Mouse Untagged Clone
CAT#: MC228109
Tcf12 (untagged) - Mouse transcription factor 12 (Tcf12), transcript variant 4
"NM_001253864" in other vectors (1)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Symbol | Tcf12 |
Synonyms | A130037E08Rik; ALF1; bHLHb20; HEB; HEBAlt; HTF-4; HTF4; ME1; REB |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC228109 representing NM_001253864
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACTGTGCTTATCCTGTCCCTGGAATGGGCAACAATTCTTTGATGTATTACTACAATGGGAAAACGG TATATGCACCATCCCCAAATTCTGACGATTTCAACCGTGAATCTCCTAGTTACCCATCTCCCAAGCCACC AACCAGTATGTTCGCTAGCACTTTCTTTATGCAAGATGGGACCCACAGTTCTTCTGACCTTTGGAGTTCA TCGAATGGGATGAGCCAGCCTGGTTTTGGTGGAATTCTGGGGACCTCCACATCCCACATGTCTCAGTCCA GTAGTTATGGCAGCCTTCATTCACATGACCGCTTGAGTTATCCTCCACACTCAGTGTCACCAACAGACAT AAACACAAGTCTTCCGCCAATGTCCAGCTTCCACCGTGGTAGTACCAGCAGCTCACCATATGTTGCCGCC TCACATACTCCTCCCATCAATGGATCAGATAGCATCCTAGGAACCAGAGGGAATGCTGCTGGAAGCTCAC AGACGGGTGATGCACTTGGGAAGGCCTTGGCATCTATTTATTCCCCTGACCACACAAGCAGTAGTTTTCC ATCAAACCCATCAACACCAGTGGGATCTCCTTCACCTCTCACAGGTACCAGTCAGTGGCCCAGAGCTGGA GGGCAAGCTCCTTCATCTCCAAGCTATGAAAACTCACTTCACTCCCTGCAGTCTCGAATGGAAGACCGCT TAGACAGGCTGGATGATGCTATCCATGTGCTACGAAACCATGCAGTTGGACCTTCTACCAGTCTGCCTAC TAGCCACAGTGACATACACAGTTTGCTGGGACCATCCCATAATGCATCAATTGGAAACCTCAATTCAAAC TATGGAGGATCCAGCCTTGTTACAAATAGTCGATCAGCTTCGATGGTCGGAACACATCGGGAAGATTCAG TCAGTCTCAATGGCAATCATTCGGTCCTGTCTAGTACTGTTGCTGCCTCAAACACAGAACTGAACCATAA AACACCAGAAAATTTCAGAGGTGGTGTACAAAATCAGTCTGGAAGTGTTGTTCCAACAGAAATCAAGACT GAAAACAAAGAAAAAGATGAAAACCTTCATGAACCTCCTTCATCAGATGACATGAAATCAGATGATGAGT CCTCCCAGAAAGACATCAAGGTCTCATCTAGGGGCAGAACAAGCAGTACCAATGAAGACGAGGATCTGAA TCCAGAACAGAAAATCGAAAGGGAGAAGGAAAGGCGGATGGCTAACAATGCCAGAGAGCGCCTGCGCGTG CGGGATATTAACGAGGCGTTCAAGGAGCTTGGCCGAATGTGTCAGCTTCATTTGAAGAGTGAAAAACCTC AGACAAAACTTCTCATTCTTCATCAGGCCGTGGCAGTCATCCTTAGTCTAGAACAGCAAGTGAGAGAGAG GAACCTCAACCCCAAAGCAGCCTGCCTTAAGAGAAGAGAAGAAGAAAAAGTCTCTGCTGCGTCAGCAGAG CCGCCCAACACGTTGCCAGGAGCCCATCCTGGGCTTAGTGAGTCTACCAACCCTATGGGTCATCTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001253864 |
Insert Size | 1539 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001253864.1, NP_001240793.1 |
RefSeq Size | 3957 bp |
RefSeq ORF | 1539 bp |
Locus ID | 21406 |
Cytogenetics | 9 39.85 cM |
Gene Summary | Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) has a different 5' end and lacks several exons in the coding region, which results in the use of a downstream start codon, compared to variant 1. The resulting protein (isoform 4) is shorter and has a distinct N-terminus when it is compared to isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR230320 | Tcf12 (myc-DDK-tagged) - Mouse transcription factor 12 (Tcf12), transcript variant 4 |
USD 524.00 |
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