Treh (NM_001277851) Mouse Untagged Clone

CAT#: MC228106

Treh (untagged) - Mouse trehalase (brush-border membrane glycoprotein) (Treh), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Treh
• Synonyms: 2210412M19Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228106 representing NM_001277851
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCTCCTGCACCAAGTTCAGATGGCCCAGCTCTACCAAGATGACAAGCAGTTTGTGGATATGTC
  ACTGGCCACATCTCCAGATGAAGTCCTGCAGAAGTTCAGTGAGCTGGCCACAGTCCACAACCACAGCATC
  CCCAAGGAACAGCTTCAGGAATTTGTCCAGAGTCACTTCCAGCCCGTGGGGCAGGAGCTGCAGTCCTGGA
  CCCCTGAGGACTGGAAGGACAGCCCTCAGTTCCTGCAGAAGATCTCGGATGCTAATCTGCGTGTCTGGGC
  GGAGGAGCTACACAAGATCTGGAAAAAGCTGGGAAAGAAGGGACTCGTACTGGGTGATGGAAGGCCTGCT
  TCTTTCTGAGATGGCCTCAACAGTGAAGGGTATGCTGCAAAACTTTCTGGATCTGGTGAAGACCTACGGA
  CATATCCCCAACGGTGGACGCATATATTACCTGCAACGGAGCCAGCCCCCACTCCTGACTCTCATGATGG
  ATCGATATGTAGCTCATACCAAGGATGTCGCCTTCCTTCAGGAGAATATTGGGACTCTAGCCTCTGAACT
  GGACTTCTGGACTGTGAACAGGACTGTCTCTGTAGTCTCAGGAGGACAAAGCTATGTCTTAAATCGCTAC
  TATGTCCCTTATGGGGGACCCAGGCCAGAGTCCTACAGGAAAGACGCAGAATTGGCAAACTCTGTGCCAG
  AAGGGGACCGAGAGACTCTGTGGGCTGAGCTCAAGGCTGGGGCTGAGTCTGGCTGGGACTTCTCTTCACG
  CTGGCTTGTTGGAGGCCCAGACCCTGATTTGCTCAGCAGCATCCGAACCAGCAAAATGGTACCCGCTGAT
  CTGAACGCGTTCCTGTGCCAAGCAGAGGAACTGATGAGTAACTTCTACTCCAGACTAGGGAACGACACAG
  AGGCCACAAAGTACAGGAACCTGCGGGCCCAGCGCTTGGCCGCCATGGAAGCTGTCCTGTGGGACGAGCA
  GAAGGGTGCCTGGTTTGACTATGACTTGGAAAAGGGGAAGAAGAACCTGGAGTTTTATCCCTCCAACCTC
  TCCCCACTTTGGGCTGGCTGCTTCTCAGACCCTAGTGTTGCTGACAAGGCTCTGAAGTACTTGGAGGACA
  GCAAGATCTTGACCTACCAATATGGAATCCCAACCTCTCTTCGTAACACAGGCCAGCAGTGGGACTTCCC
  CAATGCCTGGGCCCCACTGCAGGACCTGGTCATTAGAGGTTTGGCCAAGTCAGCTTCCCCCCGGACTCAG
  GAGGTGGCTTTCCAGCTGGCCCAGAATTGGATCAAAACCAACTTCAAAGTCTACTCCCAAAAGTCAGCGA
  TGTTTGAGAAGTATGACATCAGCAACGGTGGACATCCAGGTGGAGGAGGGGAGTATGAAGTTCAGGAAGG
  ATTTGGCTGGACAAACGGATTGGCCCTGATGCTTCTGGATCGCTATGGTGACCAGTTGACTTCAGGGACC
  CAGTTAGCTTCCCTGGGACCCCACTGCCTAGTGGCTGCCCTTCTTCTCAGTCTTCTGCTACAGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001277851
• Insert Size: 1536 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277851.1, NP_001264780.1
• RefSeq Size: 1952 bp
• RefSeq ORF: 1536 bp
• Locus ID: 58866
• Cytogenetics: 9 A5.2
• Gene Summary: This gene belongs to the alpha-glucosidase family, whose members encode enzymes that carry out hydrolysis of alpha-glucoside bonds of a variety of carbohydrates. The enzyme encoded by this gene uses the disaccharide trehalose as a highly specific substrate and converts it into two glucose molecules. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2013]
  Transcript Variant: This variant (3) differs in the 5' UTR, initiates translation at an alternate start codon and lacks an internal exon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1.